Vitamin E protected the mouse adrenal gland against immobilization stress
Yükleniyor...
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Polska Akademia Nauk
Erişim Hakkı
info:eu-repo/semantics/embargoedAccess
Özet
In this study, we aimed to investigate the effects of vitamin E on mouse adrenal glands in immobilization stress. Twenty-eight male, 10-week-old, BALB/C mice weighing 30-45 grams were divided into four groups. Mice were placed in a cage where no movement was allowed 6 hours/day for 7 days for immobilization stress. 10 ml/kg vitamin E was administered orogastrically 1 hour before immobilization stress in the vitamin E and stress+vitamin E group. At the end of the 7th day, all the animals were subjected to elevated-plus maze (anxiety) and forced swimming (depression) tests. Left adrenal glands were dissected for routine paraffin tissue embedding protocol. Adrenal sections were stained with hematoxylin-eosin and Azan. Malonaldehyde (MDA) levels were also measured in the adrenal tissues. Anxiety level (0.023), depression level (p=0.042) and MDA values (p=0.01) were significantly increased in the stress group. Histological sections of the stress group showed cortical atrophy, medullary hypertrophy, vascular dilation and hemorrhage. Azan staining revealed a thinned capsule and corticomedullary fibrosis in the stress group. Pathologies induced by immobilization stress were mostly reversed after vitamin E administration. The results suggested that vitamin E alleviates adverse effects of immobilization stress (oxidative, behavioral and histopathologic changes) in mice.
Açıklama
Anahtar Kelimeler
Adrenal gland, Immobilization, Psychopharmacological tests, Stress, Vitamin E
Kaynak
Polish Journal of Veterinary Sciences
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
25
Sayı
3
Künye
Aşır, F., Nergiz, Y. ve Pala, A. (2022). Vitamin E protected the mouse adrenal gland against immobilization stress. Polish Journal of Veterinary Sciences, 25(3), 447-454.
