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Öğe Determination of Antioxidant, Antidiabetic, Anticholinergic, Antiglaucoma Properties and Comprehensive Phytochemical Content by LC-MS/MS of Bingöl Honeybee Pollen(Wiley, 2025) İzol, Ebubekir; Turhan, Münire; Yılmaz, Mustafa Abdullah; Çağlayan, Cüneyt; Gülçin, İlhamiHoneybee pollen is the most important food and protein source for bees. It is a highly nutritious natural product for humans due to its content. Pollen shows different phytochemical content and variable biological activity according to different geography and vegetation. Therefore, in this study, the comprehensive phytochemical content by LC-MS/MS, and its antioxidant properties by different assays, with enzyme inhibition potential; antidiabetic, anticholinergic, and antiglaucoma properties, were determined from a pollen sample from Bingö l province, one of the significant beekeeping centers in Tü rkiye. As a result of LC-MS/MS, 15 metabolites were determined, and the highest concentration of quinic acid (1.531 mg analyte/g extract) was found. Antioxidant results: total phenolic content 113.14 mg GAE/g, total flavonoid content 64.11 mg QE/g, Fe3+ reduction 0.43 mu g/mL, CUPRAC 0.511 mu g/mL, FRAP 0.976 mu g/mL, DPPH IC50: 1.06 mu g/mL, ABTS IC50: 0.933 mu g/mL, and DMPD IC50: 0.598 mu g/mL. In addition, pollen showed antiglaucoma, anticholinergic, and antidiabetic properties by inhibiting carbonic anhydrase isoenzyme II (hCA II), acetylcholinesterase (AChE), and alpha- amylase enzymes, respectively. In this study, it was determined that Bingö l pollen has a comprehensive phytochemical content and antioxidant properties and, for the first time, inhibits hCA II, AChE, and alpha-amylase enzymes.Öğe Superiority of Pathologic Lymph Node Ratio over Positive Lymph Node Count in Operated Early-Stage Breast Cancer(Edizioni Luigi Pozzi, 2024) Urakçı, Zuhat; Ali Kaplan, Muhammet; Oruç, Zeynep; Gümüş, Mahmut; Uncu, Doğan; Ebinç, Senar; Özkan, MetinAIM: In early-stage breast cancer, the axillary lymph nodes play a crucial role in determining the prognosis of the disease. The rate of lymph node involvement might be a more valuable prognostic factor than the number of positive lymph nodes. Therefore, we aimed to evaluate whether the lymph node ratio (LNR) is a superior prognostic indicator compared to the pathologic lymph node count in early-stage disease. METHODS: We included 3053 non-metastatic, lymph node-positive breast cancer patients who were treated and followed at 6 medical oncology centers in Türkiye between 2004–2018. Based on LNR, patients were classified into three risk groups: high (>0.65), intermediate (0.21–0.65), and low (≤0.20). RESULTS: Classification of patients according to the TNM8 system based on the number of positive lymph nodes revealed that pathologic lymph node count (pN)1 accounted for 49.0% (n = 1495), pN2 for 30.0% (n = 917), and pN3 for 21.0% (n = 641). Based on the LNR risk group, the low-risk group accounted for 45.4% (n = 1385), intermediate for 36.2% (n = 1105), and high for 18.4% (n = 563) of the total patients. For the entire patient cohort, the 5-and 10-year disease-free survival (DFS) were 93% and 67%, respectively, while overall survival (OS) rates were 95% and 75%, respectively. The median DFS for patients with N1, N2, and N3 disease was 149 months (94.2–203.7), 120.1 months (108.2–132.0), and 81.8 months (68.4–131.1), respectively (p < 0.001). The median DFS for the three LNR risk groups (low, intermediate, and high risk) was 148.9 months (95.3–202.6), 118.7 months (99.9–137.7), and 81.8 months (68.2– 95.3) respectively. Increasing LNR rate was an independent prognostic factor for DFS, according to multivariate analysis (p < 0.001). Furthermore, the median DFS was 133 months for pathologic N1 patients in the LNR intermediate-high risk group, while the median DFS was not reached in patients with LNR and the pN2 low risk group (p = 0.034). CONCLUSIONS: This study confirms the significance of LNR as a prognostic factor for DFS. The results show that in certain specific subgroups, LNR provides more information than pathologic lymph node counts. © 2024 The Author(s).Öğe Evaluation of the Regeneration Effects of Systemically Applied Methylprednisolone and Vitamin B12 on Mental Nerve Crush Injury(Turkish Neurosurgical Society, 2024) Kara, Cigdem; Lacin, Nihat; Adak, Berat Metin; Kara, Alper; Tunik, Selcuk; Uysal, Ersin; Soylu, Fahri EmrahAIM: To evaluate the effects of methylprednisolone and vitamin B12 injection on the regeneration of the nerves after a mental nerve crush injury. MATERIAL and METHODS: A total of 40 albino Wistar rats have been randomly divided into four groups: Ynt: ÖNEMLİ: Turkish Neurosurgery_JTN 43470_proof reading group 1 (n=10): crush-like nerve damage was created by pinching the left mental nerve for 60 s with an aneurysm clip and intraperitoneally administered 1 ml of saline for 14 days; group 2 (n=10): the left mental nerve was pinched for 60 s with an aneurysm clip and intraperitoneally administered 2 mg/kg of methylprednisolone for 14 days; group 3, experimental group (n=10): the left mental nerve was pinched for 60 s with an aneurysm clip and intraperitoneally administered 2 mg/kg of vitamin B12 for 14 days; and group 4, experimental group (n=10): the left mental nerve was pinched for 60 s and intraperitoneally administered 2 mg/kg of methylprednisolone and 2 mg/kg of vitamin B12 for 14 days. All rats were sacrificed on the 28th postoperative day, and histopathological evaluation was performed. RESULTS: Nerve damage was higher in the control group than in other groups (p<0.05). When Neural Cell Adhesion Molecule (NCAM) expression levels were compared, no major differences were observed between the methylprednisolone and control groups (p>0.05). The B12 and B12+methylprednisolone groups reached significantly higher NCAM expression levels compared to the control and methylprednisolone groups. When the myelin basic protein (MBP) expression levels were compared (p<0.05), the MBP expression was significantly higher in all experimental groups than in the control group (p<0.05). CONCLUSION: Systemic vitamin B12 and methylprednisolone administration effectively supported remyelination in the crushed mental nerve by increasing Schwann cell proliferation and differentiation. © (2024), (Turkish Neurosurgical Society). All rights reserved.Öğe Evaluation of undergraduate dental students' awareness, knowledge, and attitudes towards teledentistry: does a course enhance this?(2025) Akçiçek, Gökçen; Katı, Ezgi; Zengin, Hatice YağmurBACKGROUND: Teledentistry is a part of telemedicine and can be used for dental diagnosis, dental care, consultation, and dental education. The objective of this study was to assess the awareness, knowledge, and attitudes of undergraduate dental students toward teledentistry, as well as to investigate the influence of enrolling in a teledentistry course on these variables. METHODS: A structured questionnaire-based online survey (Google Form) was used as the survey tool to evaluate the undergraduate dental students' awareness, knowledge, and attitudes towards teledentistry. All undergraduate dental students during the 2022-2023 academic year, were invited to join this questionnaire. The questionnaire was posted to the students who agreed to answer the survey online. Associations between categorical variables were evaluated using the Pearson Chi-square test or Fisher-Freeman-Halton Exact test. The Mann-Whitney U test was used to compare two independent groups, and the Kruskal-Wallis test was used to compare more than two independent groups. All analyses were conducted using IBM SPSS Statistics for Windows, Version 23.0 (IBM Corp. Released 2015, Armonk, NY: IBM Corp.). The significance level was set at p < 0.05 for all analyses. Explanatory factor analysis and item analysis were used for the evaluation of the validity of the questionnaire. RESULTS: There were 293 participants from five classes, with a 36.08% response rate; 190 (64.8%) were female and 103 (35.2%) were male, with a median age of 21 years (minimum 17 years, maximum 44 years). In this study, 37.9% of the participants had heard of teledentistry, 30.7% knew what teledentistry is, approximately one-third of the students were aware that there is a course called teledentistry in our faculty, and only 11.6% took the course. Undergraduate dental students had high levels of knowledge and awareness about the advantages and limitations of teledentistry. Dental students who had taken the course had a more positive attitude towards using teledentistry. CONCLUSIONS: Enhancing dental students' knowledge and awareness of teledentistry could beneficially influence their utilization of teledentistry in their future careers. CLINICAL TRIAL NUMBER: This study is not a clinical trial involving medicinal products or therapeutic interventions, so the clinical trial number is not applicable. © 2025. The Author(s).Öğe Ancient genomics and the origin, dispersal, and development of domestic sheep(2025) Daly, Kevin G.; Mullin, Victoria E.; Hare, Andrew J.; Halpin, Áine; Mattiangeli, Valeria; Teasdale, Matthew D.; Rossi, ConorThe origins and prehistory of domestic sheep (Ovis aries) are incompletely understood; to address this, we generated data from 118 ancient genomes spanning 12,000 years sampled from across Eurasia. Genomes from Central Türkiye ~8000 BCE are genetically proximal to the domestic origins of sheep but do not fully explain the ancestry of later populations, suggesting a mosaic of wild ancestries. Genomic signatures indicate selection by ancient herders for pigmentation patterns, hornedness, and growth rate. Although the first European sheep flocks derive from Türkiye, in a notable parallel with ancient human genome discoveries, we detected a major influx of Western steppe-related ancestry in the Bronze Age.Öğe Comparison of different fixatives effects in histochemical stainings of peripheral nerve tissue(Cellular and Molecular Biology Association, 2024) Dörtbudak, Muhammet Bahaeddin; Demircioğlu, Muhammed; Şeker, Uğur; Demircioğlu, İsmailA pathological condition in the peripheral nerve tissue, which provides the connection between the organism and the external environment, negatively affects the standard of living. The nerve tissue histotechnology is of serious importance both for scientific studies and for clinical diagnosis. The fixation, which is one of the leading procedures for histological examination of tissues, aims to preserve tissue morphology. Another essential part of the histological examination is staining process. This study, it was aimed to determine the fixative that provides optimal histological appearance in peripheral nerve tissue. Therefore, various histochemical stainings of tissues fixed with some fixatives used in practice were compared. Sciatic nerves from each rat (n=7) used in the study were fixed with different fixatives and histochemical staining was performed. In histological examination, cellular (nucleus-cytoplasm) and intercellular morphological details, staining intensity and distribution were evaluated. At the end of the study, formaldehyde was found to be the most ideal fixing agent for all stains. Although Bouin and Carnoy fixatives differed according to the staining type, their fixation quality was similar in general. Glutaraldehyde did not give as good results as other fixatives in all stainings. This study is an important technical reference for clinical and experimental studies. © 2024 Cellular and Molecular Biology Association. All rights reserved.Öğe Development of lacosamide-loaded in-situ gels through experimental design for evaluation of ocular irritation in vitro and in vivo(Elsevier B.V., 2025) Çoban, Özlem; Pınar, Sıla Gülbağ; Polat, Heybet Kerem; Gedik, Gülşah; Karakuyu, Nasıf Fatih; Pezik, Esra; Ünal, SedatLacosamide (LCM) selectively increases the slow inactivation of voltage-gated sodium channels (VGSCs) and is a N-methyl D-aspartate acid (NMDA) receptor glycine site antagonist. Therefore, it can be used in dryness-related hyperexcitability of corneal cold receptor nerve terminals. Ocular in-situ gels remain in liquid form until they reach the target site, where they undergo a sol-gel transformation in response to specific stimuli. They can show mucoadhesive properties related to the polymer used and increase the residence time of the drug in the mucosa. In the presented study, ocular in-situ gel formulation of LCM, which has potential for use in ocular diseases and consists of hyaluronic acid and poloxamer 407 as polymers, was developed using cold method. The effect of formulation components on target product properties (pH, gelation temperature and viscosity) was evaluated by design of experiments (DoE) design. The optimized LCM-loaded in-situ gel had a pH value of 6.90 ± 0.01, showed pseudo-plastic flow with a viscosity of 562 ± 58 cP at 25 °C, gelled at 33 ± 0.47 °C, and released drugs via the Peppas-Sahlin mechanism. Ocular safety was confirmed via in vitro tests using two different cell lines (L929 and Arpe-19), along with in vivo Draize tests, histological examinations, and Hen's Egg Chario-Allontioc-Membrane (HET-CAM) analysis. In vitro studies confirmed the optimized LCM-loaded in-situ gel's suitability for ocular use, demonstrating long-acting effects through controlled release. In addition, ocular irritation and histological studies have supported that it will not show any toxic effect on the eye tissue. © 2024 American Pharmacists AssociationÖğe Exploring 18F-FDG uptake patterns in liver, spleen, and bone marrow: Implications for inflammatory and infectious conditions(2024) Tuzcu, Şadiye Altun; Çetinkaya, Erdal; GÇzel, Fatih; Çetin, İlbey Erkin; Mete, Ümit; Argın, Eşref; Taşdemir, BekirOBJECTIVE: This study aimed to explore the relationship between fluorine-18-fluorodeoxyglucose (18F-FDG) uptake in the liver, spleen, and bone marrow and inflammatory markers such as c-reactive protein (CRP), albumin, and erythrocyte sedimentation rate (ESR) in patients undergoing positron emission tomography/computed tomography (PET/CT) imaging for cancer diagnosis. SUBJECTS AND METHODS: This retrospective cross-sectional study included a total of 708 patients with a diagnosis of malignancy. Fluorine-18-FDG PET/CT images acquired between January 2021 and December 2022. Exclusion criteria comprised prior chemotherapy, radiotherapy, hematological malignancies, or liver/spleen tumors. Statistical analysis included correlation analysis, univariate, and multivariate regression analysis. RESULTS: C-reactive protein levels demonstrated a significant positive correlation with 18F-FDG uptake in the spleen (r=0.104, P=0.006) and bone marrow (r=0.112, P=0.003). Albumin showed a negative correlation with liver 18F-FDG uptake (r=-0.220, P<0.001). Regression analysis revealed ESR's impact on spleen-to-liver (P=0.023) and bone marrow-to-liver (P=0.012) 18F-FDG uptake. CONCLUSION: This study demonstrates the association between inflammatory markers and 18F-FDG uptake in liver, spleen and bone marrow. C-reactive protein and ESR showing significant correlations with spleen and bone marrow 18F-FDG uptake, and albumin correlated with liver 18F-FDG uptake negatively. Erythrocyte sedimentation rate had significant impact on spleen and bone marrow 18F-FDG uptakes. These findings suggest the potential of 18F-FDG PET/CT in diagnosing inflammatory conditions, warranting further investigation into its clinical implications.Öğe Association Between ABO Blood Group, Peripheral Artery Disease Lesion Severity, and Coronary Artery Disease Coexistence(Elsevier Inc., 2025) Evsen, Ali; Aktan, Adem; Kılıç, Raif; Özbek, MehmetBackground: This study aims to investigate the relationship between ABO blood groups and the severity of peripheral artery disease (PAD) lesions, the coexistence of coronary artery disease (CAD) with PAD, and to identify which blood groups may be more predisposed to these conditions. Methods: This study, which has a single-center and retrospective design, includes 305 patients diagnosed with peripheral artery disease (PAD) between 2015 and 2021. The patients were selected from those with at least 50% stenosis detected by computed tomography (CT) angiography. The severity of PAD lesions was evaluated according to the TransAtlantic Inter-Society Consensus (TASC-II) classification, and the presence of CAD was determined by simultaneous conventional coronary angiography. Demographic data, comorbidities, and laboratory results were collected, and statistical analyses were performed using chi-square tests, logistic regression, and t-tests. All of these patients were divided into 2 groups based on their ABO blood types: O and non-O blood groups. Results: This study demonstrates a significant relationship between blood group classification and both the severity of PAD and the prevalence of CAD. Patients with non-O blood groups exhibited a higher likelihood of having severe PAD lesions (TASC-C and TASC-D) compared to those with O blood groups, who more frequently had milder lesions (TASC-A and TASC-B) (P < 0.001). The prevalence of CAD was also significantly higher among patients with non-O blood groups than those with the O blood group (54.4% vs. 36.4%; P = 0.003). In the logistic regression analysis, non-O blood groups, alongside age, hypertension (HT), and chronic kidney disease (CKD), emerged as independent predictors of severe PAD (P < 0.001 for non-O blood groups). Similarly, older age and non-O blood group status were identified as significant independent predictors of CAD (P = 0.004). These findings suggest that blood group classification, particularly non-O groups, may influence the severity and coexistence of PAD and CAD. Conclusion: Our study reveals a significant association between ABO blood groups and both the severity of PAD and the association of CAD, and shows that non-O blood groups are linked to more severe forms of these conditions. These findings highlight the potential role of ABO blood groups in cardiovascular risk stratification, suggesting a need for further research to confirm these associations and understand their clinical implications. © 2025 Elsevier Inc.Öğe Exploring 18 F-FDG uptake patterns in liver, spleen, and bone marrow: Implications for inflammatory and infectious conditions(Hellenic Soc Nuclear Medicine, 2024) Tuzcu, Sadiye Altun; Cetinkaya, Erdal; Guzel, Fatih; Cetin, lbey Erkin; Mete, Umit; Argin, Esref; Tasdemir, BekirObjective: This study aimed to explore the relationship between fluorine-18-fluorodeoxyglucose (F-18-FDG) uptake in the liver, spleen, and bone marrow and inflammatory markers such as c-reactive protein (CRP), albumin, and erythrocyte sedimentation rate (ESR) in patients undergoing positron emission tomography/computed tomography (PET/CT) imaging for cancer diagnosis. Subjects and methods: This retrospective cross-sectional study included a total of 708 patients with a diagnosis of malignancy. Fluorine-18-FDG PET/CT images acquired between January 2021 and December 2022. Exclusion criteria comprised prior chemotherapy, radiotherapy, hematological malignancies, or liver/spleen tumors. Statistical analysis included correlation analysis, univariate, and multivariate regression analysis. Results: C-reactive protein levels demonstrated a significant positive correlation with F-18-FDG uptake in the spleen (r=0.104, P=0.006) and bone marrow (r=0.112, P=0.003). Albumin showed a negative correlation with liver F-18-FDG uptake (r=-0.220, P<0.001). Regression analysis revealed ESR's impact on spleen-to-liver (P=0.023) and bone marrow-to-liver (P=0.012) F-18-FDG uptake. Conclusion: This study demonstrates the association between inflammatory markers and F-18-FDG uptake in liver, spleen and bone marrow. C-reactive protein and ESR showing significant correlations with spleen and bone marrow F-18-FDG uptake, and albumin correlated with liver F-18-FDG uptake negatively. Erythrocyte sedimentation rate had significant impact on spleen and bone marrow F-18-FDG uptakes. These findings suggest the potential of F-18-FDG PET/CT in diagnosing inflammatory conditions, warranting further investigation into its clinical implications.Öğe Prognostic importance of nutritional assessment in patients with acute ischemic stroke undergoing endovascular thrombectomy(Verduci Publisher, 2023) Ozbek, M.; Akil, M. A.; Demir, M.; Arik, B.; Demir, F.; Akil, E.- OBJECTIVE: The prevalence of malnutrition in patients with acute isch-emic stroke (AIS) can range from 8% to 34%. It has been shown that prognostic nutritional in-dex (PNI) and control nutritional status (CO -NUT) scores can provide an opportunity to make prognostic predictions in some disease groups. Previous studies have shown a close relationship between malnutrition scores and stroke prognosis. We evaluated the effect of nutritional scores on in-hospital and long-term mortality in AIS patients undergoing endovas-cular therapy (EVT).PATIENTS AND METHODS: 219 patients who underwent EVT for the AIS were included in this retrospective design and cross-sectional study. The primary endpoint of the study was accepted as all-cause death including in-hospital death, 1-year death, and 3-years death. RESULTS: A total of 57 patients died in the hospital. In-hospital mortality rate was higher in the high CONUT group [36 (49.3%), 10 (13.7%), 11 (15.1%), p<0.001]. A total of 78 patients died within one year, and 1-year mortality was higher in the high CONUT group [43 (58.9%), 21 (28.8), 14 (19.2), p<0.001]. At the end of the 3-year fol-low-up, 90 patients had died, and the 3-year mortality rate was significantly higher in groups with a high CONUT score than in those with a low CONUT score (p<0.001).CONCLUSIONS: A higher CONUT score, cal-culated easily by simple scoring with parame-ters studied from peripheral blood before the EVT procedure, is an independent predictor of in-hospital, 1-year, and 3-years all-cause mor-tality.Öğe The Hepatic Phase of Fascioliasis Complicated with Liver Bleeding: Presentation of Three Cases(Iranian Scientific Society Medical Entomology, 2024) Ucmak, Feyzullah; Kaya, MuhsinBleeding is a very rare complication of Fasciola species ( F. hepatica or F. gigantica ) infection. We present here three cases of subcapsular liver bleeding caused by the hepatic phase of Fasciola spp. infection in patients, two of whom were women, aged 22, 66, and 84 years in Diyarbakir Province, southeastern Turkey. They had symptoms of right upper quadrant pain (n = 3), nausea (n = 1), and vomiting (n = 2) for periods ranging from 6 hours to 15 days. All patients with clinical presentations ranging from moderate abdominal pain to hypovolemic shock and ischemic hepatitis were improved with supportive treatment without the need for surgery. They showed complete clinical and laboratory recovery after triclabendazole administration in their follow-up. In conclusion, Fasciola spp . infection should be considered in the etiology of bleeding from liver disease.Öğe Phytochemical Analysis and Assessment of Antioxidant and Enzyme Inhibitory Activity of Alchemilla pseudocartalinica Juz.(Wiley-V C H Verlag Gmbh, 2024) Uzunkaya, Cagla; Gokkaya, Icim; Akkaya, Didem; Soral, Michal; Seyhan, Gokce; Barut, Burak; Yilmaz, Mustafa AbdullahThis study aimed to evaluate and compare the antioxidant capacity and enzyme inhibitory activity of extracts, sub-extracts, and fractions prepared from the aerial parts and roots of A. pseudocartalinica. The phytochemical content of the active extracts was also analyzed. According to the results, ellagic acid (38.42 mg/g) was the major compound in the aerial part methanol extract and catechin (185.30 mg/g) in the root methanol extract. The DPPH inhibition activity of all fractions was monitored, with the most active one (Fr B) reaching an IC50 value of (4.92 +/- 0.59 mu g/mL). All the fractions prepared from the aerial parts' water sub-extract showed higher a-glucosidase inhibitory activity than the positive control acarbose. In the tyrosinase assay, Fr B (58.81 +/- 7.50 mu g/mL) exhibited the highest inhibitory actions among all fractions. The structure of the major substances of the most active fraction were elucidated as quercetin 7-O-beta-glucopyranosyl-3-O-beta-glucuronopyranoside- (1), and alpha-[(2-formyl-5-hydroxymethyl)pyrrol-1-yl]aspartic acid (2).Öğe Phytochemical Profiling by UHPLC-ESI-MS/MS, In Vitro Antioxidant, In Vivo Antidiabetic, and Pro-Sexual Effects of Salvia balansae Noë Ex Coss. Flower Extract in Diabetic Male Rats(Wiley-V C H Verlag Gmbh, 2025) Souidi, Wadjila S.; Belhocine, Mansouria; Bouzouina, Mohamed; Yilmaz, Mustafa A.; Feknous, Ines; Cakir, Oguz; Tarhan, AbbasOur study assessed the phytochemical composition and the effects of Salvia balansae flower aqueous extract (FAE) on sexual dysfunction in diabetic rats. Total phenolic and flavonoid content, quantification of phytochemicals by ultrahigh performance liquid chromatography (UHPLC)-electrospray ionization (ESI)-tandem mass spectrometry (MS/MS) method, and in vitro antioxidant capacity of FAE extract were elucidated. For the in vivo study, diabetes was induced by one intraperitoneal injection of streptozotocin (STZ) (40 mg/kg), and treated diabetic rats were given FAE at 200 mg/kg. Rats were subjected to a sexual incentive motivation test (SIM) and a mating behavior test, and then serum testosterone levels and sperm quality were analyzed. Our study indicates that FAE contained a large amount of phenolic compounds and flavonoids, and 13 constituents were quantified; cynaroside (3.8 mg analyte/g extract) was the main compound, followed by rosmarinic acid (3.145 mg analyte/g extract), thus confirming its high antioxidant capacity. FAE treatment significantly reduced blood glucose levels, improved sexual motivation and copulatory behavior, increased testosterone levels, and enhanced sperm quality in diabetic-treated rats compared to diabetic untreated rats. The present work demonstrates the therapeutic potential of S. balansae flowers in enhancing reproductive function in diabetic rats, potentially through its antioxidant, antihyperglycemic, and aphrodisiac properties.Öğe Optimizing pectin lyase production using the one-factor-at-a-time method and response surface methodology(Wiley, 2024) Gul, Ertugrul; Dursun, Arzu Yadigar; Tepe, Ozlem; Akaslan, Gonca; Pampal, Fadile GulPectinases are commonly industrially synthesized by molds. This study aimed to optimize pectin lyase synthesis by a bacterium, Pseudomonas fluorescens, using both the one-factor-at-a-time (OFAT) method and response surface methodology. First, on optimization of pectin lyase fermentation by the OFAT method, the effects of pectin, peptone, yeast extract, (NH4)(2)SO4, pH, and salts were investigated. The highest pectin lyase activity was determined to be 28.63 U/mL at pH 8, 30 degrees C, with 1% (w/v) pectin and 0.14% (w/v) (NH4)(2)SO4 concentration at the 90th hour. The effect of substrate inhibition on the microbial growth was also investigated, and the results showed that the process can be described by noncompetitive inhibition model. The values of kinetic constants were determined as mu (m) = 0.175 h(-1), K-S = 6.931 g/L, and, K-I = 6.932 g/L by nonlinear regression analysis. It was reported that pectin lyase enzymes exhibited peak activity at 50 degrees C and pH 8. Finally, response surface methodology (RSM) was utilized to optimize pH, concentrations of ammonium sulfate, and pectin, which were chosen as independent variables. The interactions between these variables were also examined. According to RSM, the optimum values of the parameters to achieve a maximum pectin lyase activity of 35.62 U/mL were determined to be pH 7.97, 1.25% (w/v) pectin concentration, and 0.25% (w/v) (NH4)(2)SO4 concentration.Öğe LC-HRMS Profiling of Phytochemicals with Assessment of Antioxidant, Anticholinesterase, and Antimicrobial Potentials of Astragalus Brachystachys DC(Wiley-V C H Verlag Gmbh, 2024) Ersoy, Ezgi; Boga, Mehmet; Kaplan, Alevcan; Kara, Emel Mataraci; Ozkan, Esra Eroglu; Kayiran, Serpil DemirciAstragalus species are ubiquitous in the pharmacopeia of numerous countries, signifying their widespread medicinal applications. T & uuml;rkiye is esteemed as one of the principal epicenters of diversity for this genus with 483 taxa, and many of these plants have been traditionally utilized for diseases including coughing, diabetes, cardiovascular disorders, and aches. Although there is a considerable body of chemical and biological research available on several Astragalus species, studies focusing on Astragalus brachystachys DC are exceedingly rare. In this context, This study provides the first comprehensive report on this medicinal plant the chemical constituents and biological activities of an important medicinal plant, Astragalus brachystachys DC. The aerial part samples were collected from Adana, T & uuml;rkiye, and an ethanol extract was prepared with these parts. The secondary metabolites of the extract were determined by an LC-HRMS analysis. The LC-HRMS analysis showed the presence of 39 different constituents, hyperoside (303.419 +/- 10.50 mu g/g extract), p-coumaric acid (256.975 +/- 8.51 mu g/g extract), and rutin (72.684 +/- 2.23 mu g/g extract) were determined as major compounds in the aerial parts ethanol extract. Attributed to its high total phenolic (58.53 +/- 1.30 mu g PEs/mg extract) and total flavonoid content (29.98 +/- 0.83 mu g QEs/mg extract), the extract demonstrated strong antioxidant activity according to three different assays namely DPPH free (IC50: 33.08 +/- 0.61 mu g/mL), and ABTS cation radical scavenging (IC50: 15.39 +/- 0.72 mu g/mL) and CUPRAC activity (A0.5: 36.25 +/- 0.28 mu g/mL) methods. In vitro assays showed that cholinesterase inhibitory activity results were found to be exceptional with 85.95 +/- 0.52 % inhibition on acetylcholinesterase and 66.32 +/- 1.33 % inhibition on butyrylcholinesterase at 200 mu g/mL. Regarding antimicrobial properties, Astragalus brachystachys DC extract was found to be effective against Enterococcus faecalis with a MIC value of 39.06 mu g/mL.Öğe Predictors of Traumatization Among Humanitarian Aid Workers Working With Refugees and Asylum Seekers in Türkiye(Wiley, 2025) Altinoglu-Dikmeer, Ilkiz; Ergul-Topcu, Aysun; Karaaslan, Cemrenur; cakmak-Tolan, OzlemRefugees and asylum-seekers flee their countries due to conflict, violence or persecution and seek safety in another country. Professionals working with refugees in host countries are being exposed to the narratives of their traumatic experiences, an important risk factor for vicarious posttraumatic stress. The current study examined predictors for traumatic stress in humanitarian aid workers working with refugees and asylum seekers in T & uuml;rkiye. We surveyed 156 participants (67.3% women) from various disciplines (psychologists, social workers, interpreters, lawyers etc.) to examine their traumatic stress, resilience and stress coping mechanisms. We used Secondary Traumatic Stress Scale (STSS), Adult Resilience Measure (ARM) and Coping Styles Inventory (CSI) for data collection. In this cross-sectional study, data were collected online from participants who were contacted through snowball sampling method, starting with the participants working with refugees at public agencies and NGOs. The regression analyses showed that the level of traumatic stress is predicted by feelings of fatigue, number of years worked with the refugees, presence of previous psychological problems and being a social worker. The relational resources subdimension of resilience and helpless coping style are also significantly associated with traumatic stress. Considering the rapidly developing phenomenon of migration all over the world, the findings of the current study could contribute to improve protective and preventive interventions for the psychological wellbeing of humanitarian aid workers.Öğe Inhibition of Nε-(carboxyethyl)lysine and Nε-(carboxymethyl)lysine formation in beef, chicken, and fish meat: A comparative study of oven frying and air frying with a marinade-containing Micromeria fruticosa(Wiley, 2024) Altun, Serap Kilic; Aydemir, Mehmet Emin; Takim, Kasim; Yilmaz, Mustafa AbdullahThe objective of this study was to assess the impact of marinating beef, chicken, and fish with Micromeria fruticosa (M. fruticosa) on the inhibition of N-epsilon-(carboxyethyl)lysine (CEL) and N-epsilon-(carboxymethyl)lysine (CML). Furthermore, our objective was to examine how different cooking techniques, temperatures, and durations affect the creation of CEL and CML in these meat products. The study began with the characterization of M. fruticosa. Subsequently, meat samples were marinated using an M. fruticosa-containing marinade and stored at 4 +/- 1 degrees C for 24 h. Following storage, the meats underwent cooking in an oven at 200 degrees C for 12 min and in an air fryer at 250 degrees C for 8 min. Subsequently, pH, color, thiobarbituric acid reactive substances (TBARS), as well as CEL and CML analyses were conducted. M. fruticosa had high levels of biological activity and bioactive content. Moreover, increasing the M. fruticosa ratio in the marinade demonstrated a reduction in TBARS, CML, and CEL formation. This study concludes that M. fruticosa can be effectively used as a marinade component for meat, inhibiting the formation of CEL and CML. In conclusion, this research underscores the significant potential of M. fruticosa in reducing the synthesis of advanced glycation end products (AGEs) during meat processing. These results not only enhance our comprehension of the complex relationship between plant extracts and meat quality but also present encouraging prospects for fostering healthier and safer cooking methods.Öğe Emphysematous hepatitis: A rare fatal case(Wiley, 2024) Tekinhatun, Muhammed; Yavas, Hueseyin GokhanEmphysematous hepatitis (EH) is a rare, insidious, rapidly progressing, and often fatal liver infection characterized by diffuse air in the liver parenchyma. While infectious parenchymal diseases can affect many intra-abdominal organs such as the kidney, urinary bladder, gall bladder, stomach, and pancreas, liver involvement is uncommon. Few cases of EH have been reported in the literature, with only four successfully treated. Diagnosis involves patient history, clinical and laboratory findings, and computed tomography. Treatment is challenging and requires close monitoring. This case report aims to enhance the understanding of EH's diagnosis and treatment in medical literature. Emphysematous hepatitis (EH) is a very rare and fatal liver infection with diffuse air in the parenchyma. The diagnosis of EH, can be made by patient history, clinical and laboratory findings, and computed tomography. We aimed to contribute to the literature on the diagnosis and treatment process of EH. imageÖğe Is a two-hour monitoring period sufficient and safe for patients undergoing ultrasound-guided percutaneous liver mass biopsy?: A prospective and multicenter experience(Wiley, 2024) Aslan, Halil Serdar; Arslan, Muhammet; Alver, Kadir Han; Demirci, Mahmut; Korkmaz, Mehmet; Esen, Kaan; Turmak, MehmetPurposeTo investigate whether patients undergoing percutaneous liver mass biopsy (PLMB) can be safely discharged following a two-hour monitoring period.MethodsA multi-center prospective analysis was conducted for 375 patients (196 males and 179 females), mean age 63 +/- 12.45 years (range 37-89) who underwent PLMB between August 2023 and March 2024. Patients were monitored for 24 h, and complications were classified as minor or major. The timing of complications was categorized into three groups: within the first two hours, between the 2nd and 24th hours, and within 1 week after 24 hours.ResultsMinor complications occurred in 18.93% (71/375) and major complications in 2.13% (8/375). Most minor complications (80.2%, 57/71) appeared within the first two hours, 12.7% (9/71) between 2 and 24 h, and 7.1% (5/71) after 24 h. All major complications (62.5%, 5/8) except late-onset cases, occurred within the first two hours. No major complications occurred between 2 and 24 h. Late-onset major complications occurred in 37.5% (3/8) after 24 h.ConclusionThe two-hour monitoring period did not adversely impact patient management regarding minor complications and is safe for identifying all major complications except for late-onset ones. Extending the post-biopsy recovery period does not significantly improve patient care. A two-hour observation period after PLMB, including hemogram monitoring and ultrasound, does not adversely affect patient management regarding minor complications and is safe and efficient for detecting significant complications except delayed ones. Extending recovery time does not significantly improve patient care, as delayed complications appear later. image
