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Öğe Aksiyal spondiloartrit hastalarında serum endokan seviyesinin ve hastalık aktivitesiyle ilişkisinin araştırılması(Dicle Üniversitesi Tıp Fakültesi, 2023) Gündüz, Sevgi; Gündüz, İbrahim; Kaplan, İbrahim; Çağlayan, MehmetAmaç: Spondiloartrit (SpA) terimi; ortak klinik, genetik, laboratuvar ve radyolojik özellikleri olan bir grup hastalığı tanımlayan ortak bir terimdir. Hastaları takipte kullanılabilecek duyarlı bir biyobelirteç maalesef yoktur. Yapılan çalışmalarla endokanın akut ve kronik enflamasyonun değişik basamaklarında önemli görevler üstlendiği gösterilmiştir. Biz bu çalışmada aksiyel SpA (axSpA) tanılı hastalarda serum endokan düzeylerini, bu düzeylerin klinik ve laboratuvar parametreler ile korelasyonunu araştırmayı amaçladık. Yöntemler: Çalışmaya “Assessment of SpondyloArthritis International Society (ASAS)” tarafından axSpA için geliştirilmiş ASAS sınıflandırma kriterlerine göre tanı almış axSpA hastaları ve bilinen hastalığı olmayan sağlıklı gönüllüler alındı. Demografik, klinik özellikleri ve laboratuvar verileri kayıt edildi. Eş zamanlı alınan serumda endokan düzeyleri “enzyme linked immunosorbent assay (ELİSA)” ile ölçüldü. Bulgular: Spondiloartrit grubu ile kontrol grubu arasında serum endokan düzeyleri arasında istatistiksel açıdan anlamlı farklılık görülmedi (p: 0,084). Alt grup analizinde de 3 grup arasında istatistiksel açıdan anlamlı farklılık görülmedi (p:0,175). Serum endokan düzeyi ile hemoglobin düzeyi arasında pozitif korelasyon (r:0,335 ve p:0,009), eritrosit sedimentasyon hızı (ESH) arasında negatif korelasyon (r:-0,32 ve p:0,010) görüldü. Serum endokan düzeyleri ile sabah tutukluğu süresi arasında anlamlılık düzeyine yaklaşan negatif korelasyon görüldü (r: -0,294 p: 0,022). Sonuç: Serum endokan düzeyleri değerlendirildiğinde, hasta ve kontrol grubu arasında istatistiksel açıdan anlamlı farklılık görülmedi. Serum endokan düzeyleri bazı laboratuvar parametreleri arasında korelasyon görüldü. Bu moleküllerin axSpA patogenezindeki rolünün, tanı ve takipte kullanılmasının yerinin daha iyi anlaşılması için daha kapsamlı klinik çalışmalara ihtiyaç olduğunu düşünüyoruz.Öğe Alternative methods for the diagnosis of macroprolactinemia: urine prolactin level and serum / urine prolactin ratio(Dicle Üniversitesi Tıp Fakültesi, 2019) Pekkolay, Zafer; Tuna, Mazhar Müslüm; Güven, Mehmet; Tuzcu, Şadiye Altun; Kaplan, İbrahim; Akkuş, Zeki; Tuzcu, Alpaslan KemalObjective: The aim of this study was to investigate the urine prolactin and serum/urine prolactin ratio for accuracy diagnosis of macroprolactinemia. Methods: In the retrospective cross-sectional analysis, prolactin levels (high or normal) in the reproductive period of men and women were included in the study. Polyethylene glycol (PEG) precipitation method was used for the detection of macroprolactinemia. Then, patients were divided into three groups as macroprolactinemia, prolactinoma and healthy control group. In patients, prolactin values in spot urine with simultaneous serum prolactin values were calculated. The non-parametric Kruskal-Wallis test was used to compare the groups. The receiver-operating characteristic (ROC) curve was determined to evaluate the predictive power of serum/urine prolactin ratio Results: A total of 41 patients were included in the study. Female/male:36 (87.8%)/ 5 (12.2%). Urinary prolactin median(minimum-maximum) values were macroprolactinemia, prolactinoma and control group, respectively; 0.06(0.05-0.10), 0.11(0.02-0.95), 0.08(0.05-0.25) ng/ml. Serum/urine ratio median (minium-maximum) values were macroprolactinemia, prolactinoma, and control group, respectively; 633(51-1032), 990(104-9635), 395.5(138-953). When the groups were compared, the patients with prolactinoma had higher urinary prolactin levels(p <0.01). Serum/urine prolactin ratio was found to be the highest in prolactinoma patients and the lowest in the control group and a significant difference was observed in groups (p <0.01). ROC analysis(control-macroprolactin) for serum/urine prolactin ratio (Sensitivity 84.6 specificity 93.7 cut off >549,5 AUC=0.83) p<0.01 Conclusion: Urinary prolactin level and serum/urine prolactin ratio may be used in diagnosis of macroprolactinemia.Öğe Are serum Netrin-4 levels predictive of preeclampsia?(Via Medica, 2020) İçen, Mehmet Sait; Agaçayak, Elif; Fındık, Fatih Mehmet; Tunç, Senem Yaman; Kaplan, İbrahim; Tan, İlhan; Evsen, Mehmet Sıddık; Gül, TalipObjective: To investigate the levels of anti-angiogenic factors, namely sFlt-1 and Netrin-4, in patients with preeclampsia (PE). Material and methods: Cord-blood (UC) sFlt-1 and Netrin-4 concentrations were measured in 30 patients with severe PE, 30 patients with PE and 30 control infants and their mothers (MS). Results: Maternal sFlt-1 levels were significantly higher in the severe PE and PE groups than in the control group. There were no statistical differences among the three groups in maternal and fetal Netrin-4 levels. But Netrin-4 levels were found to be the lowest in the control group and higher in the PE and severe PE groups. The correlation analysis revealed a positive correlation between maternal sFlt-1 levels and maternal Netrin-4 levels (p = 0.012, and r = 0.263), maternal sFlt-1 levels and fetal sFlt-1 levels (p = 0.012, and r = 0.263). Conclusions: There was a positive correlation found between maternal sFlt-1 levels and maternal Netrin-4 levels. We are of the opinion that elevation in levels of Netrin-4 might be secondary to placental hypoxia occurring in PE. The present study led to the consideration of anti-angiogenic biomarkers (sFlt-1 and Netrin-4) on automated platforms for clinical use as an aid in establishing the diagnosis and prognosis of PE.Öğe Can paricalcitol increase the effectiveness of N-acetylcysteine in contrast induced acute kidney prophylaxis in rats? A biochemical and histopathological study(Universidad de la Frontera, 2022) Yıldırım, Yaşar; Bahadır, Veysi; Aydın, Emre; Aydın, Fatma Yılmaz; Yılmaz, Zülfükar; Ketani, Aydın; Kaplan, İbrahim; Tuncer, Mehmet Cudi; Kadiroǧlu, Ali Kemal; Yılmaz, Mehmet EminN-Acetylcysteine (NAC) is used for contrast induced acut kidney injury (CI-AKI) prophylaxis because of its antioxidant effects. Paricalcitol, which has reno-protective effects, is likely to provide a more effective prophylaxis when added to NAC treatment. The study was designed based on this hypothesis. The study was organised to include 4 groups each consisting of 7 rats. Group 1 was the control group, and Group 2 included rats with CI-AKI. Rats in Group 3 were administered NAC at a dose of 100 mg/kg via oral gavage once a day for 5 days. Rats in group 4 were administered paricalcitol at a dose of 0.4 mcg/kg once a day for 5 days in addition to NAC. CI-AKI was induced after the treatments in both groups. The study was terminated on the sixth day. Samples were collected from the rats’ sera and kidney tissues to study oxidant and antioxidant parameters; kidney function tests were also studied. There were significant differences between the contrast nephropathy group (Group 2) and NAC and NAC+paricalcitol groups with respect to serum urea and creatinine levels. When the same groups were compared regarding oxidant (TOS-MDA) and antioxidant (TAC-Paraoxonase) parameters, we observed that the oxidant parameters increased in serum and kidney tissue samples with NAC use, and that effect was strengthened by the addition of paricalcitol to NAC treatment. However, despite increased antioxidant effectiveness, we observed no decrease in urea and creatinine levels when paricalcitol was added for CI-AKI in rats. There was no significant difference between Group 3 and Group 4. Paricalcitol provides a more potent antioxidant effect in both serum and kidney tissue samples when added to NAC treatment in rats with CI-AKI. Despite increased antioxidant parameters, however, paricalcitol does not provide a significant decrease in urea and creatinine levels.Öğe Carvacrol and pomegranate extract in treating methotrexate-induced lung oxidative injury in rats(International Scientific Literature Inc., 2014) Şen, Hadice Selimoǧlu; Şen, Velat; Bozkurt, Mehtap; Türkçü, Gül; Güzel, Abdulmenap; Sezgi, Cengizhan; Abakay, Özlem; Kaplan, İbrahimBackground: This study was designed to evaluate the effects of carvacrol (CRV) and pomegranate extract (PE) on methotrexate (MTX)-induced lung injury in rats.Material/Methods: A total of 32 male rats were subdivided into 4 groups: control (group I), MTX treated (group II), MTX+CRV treated (group III), and MTX+PE treated (group IV). A single dose of 73 mg/kg CRV was administered intraperitoneally to rats in group III on Day 1 of the investigation. To group IV, a dose of 225 mg/kg of PE was administered via orogastric gavage once daily over 7 days. A single dose of 20 mg/kg of MTX was given intraperitoneally to groups II, III, and IV on Day2. The total duration of experiment was 8 days. Malondialdehyde (MDA), total oxidant status (TOS), total antioxidant capacity (TAC), and oxidative stress index (OSI) were measured from rat lung tissues and cardiac blood samples.Results: Serum and lung specimen analyses demonstrated that MDA, TOS, and OSI levels were significantly greater in group II relative to controls. Conversely, the TAC level was significantly reduced in group II when compared to the control group. Pre-administering either CRV or PE was associated with decreased MDA, TOS, and OSI levels and increased TAC levels compared to rats treated with MTX alone. Histopathological examination revealed that lung injury was less severe in group III and IV relative to group II.Conclusions: MTX treatment results in rat lung oxidative damage that is partially counteracted by pretreatment with either CRV or PE.Öğe Comparison of the effect of periodontal therapy and diode decontamination and periodontal therapy alone on the amount of TNF-alpha in systemically healthy chronic periodontitis patients a non-randomized clinical trial(SCI Printers & Publication INC, 2020) Doğru, Arzum Güler; Toprak, Ömer; Üner, Devrim Deniz; İzol, Bozan Serhat; Kaplan, İbrahim; Doğru, Mehmet; İpek, FikretAbstract OBJECTIVE: To evaluate the outcome of initial periodontal therapy and additional diode laser decontamination therapy on clinical parameters and the amount of tumor necrosis factor alpha (TNF-alpha) in gingival crevicular fluid (GCF) in patients with chronic periodontitis. STUDY DESIGN: The study included 22 patients who were diagnosed with chronic periodontitis and who had at least 1 (>= 5 mm) periodontal pocket in the posterior region of each upper left hemi-arcade. The study was designed as a split-mouth study involving mechanical therapy on the right side and nonsurgical periodontal therapy plus diode laser decontamination therapy on the left side. RESULTS: A statistically significant decrease was observed in clinical parameters at 3 months when compared to the values at baseline in both treatment areas. The change in clinical parameters at 3 months after therapy was not significantly different between the groups (p>0.05). The comparison of GCF TNF-alpha levels at baseline and at 3 months in the 2 treatment areas identified significant changes in the TNF-alpha levels in the hemi-arcade treated with laser diode contamination therapy, whereas the changes in the hemi-arcade treated with nonsurgical periodontal therapy were not significant (p>0.05). A between-group comparison revealed no statistically significant difference at 3 months post-therapy, while the difference at 1 month was found to be statistically significant. CONCLUSION: The study found that laser therapy in addition to nonsurgical periodontal therapy has a positive effect on the clinical parameters and proinflammatory TNF-alpha levels in the early period but makes no contribution in the long term.Öğe The contribution of postnatal steroid administration to early brain damage in preterm babies with bronchopulmonary dysplasia(TÜBİTAK, 2021) Ertuğrul, Sabahattin; Darakci, Savaş Mert; Kaplan, İbrahim; Yolbaş, İlyas; Değer, İbrahim; Yılmaz, Sibel Tanrıverdi; Aktaş, ŞerafettinBackground/aim: Postnatal corticosteroids are commonly used to treat bronchopulmonary dysplasia (BPD). We aimed to show whether S100 calcium-binding B (S100B), neuron-specific enolase (NSE), Tau protein or microtubule-associated protein tau (MAPT), and glial fibrillary acid protein (GFAP) levels would provide any evidence of early neurological damage in premature infants receiving postnatal low dose dexamethasone therapy for BPD treatment. Materials and methods: In this cohort study, 136 preterm infants diagnosed with BPD at ≤32 weeks of gestation formed the study group, and 64 preterm infants formed the control group. NSE, S100B, GFAP, and MAPT levels were first measured before the postnatal corticosteroid treatment in both the patient and the control group on the 28th day and, for a second time, after treatment termination in the patient group. Results: There were significant differences between the measured GFAP, MAPT, and NSE values of the BPD and control groups on the 28th day, whereas there was no significant difference between the measured S100B values of the two groups. There were a statistically significant difference between the NSE values measured on the 28th day and after the treatment within the BPD group, whereas no significant difference existed between the GFAP, MAPT, and S100B values. Conclusion: NSE levels, which indicate brain damage in the early period, increased in preterm babies with BPD who had been administered postnatal dexamethasone.Öğe Depolama Sıcaklığı ve Süresinin Serum Lityum ve Total Valproik Asit Düzeylerinin Ölçüm Sonuçlarına Etkisi(2014) Arslan, Rahile; Çolpan, Leyla; Toprak, Gülten; Yüksel, Hatice; Kaplan, İbrahim; Evliyaoğlu, OsmanAmaç: Lityum ve valproik asit psikiyatrik ve nörolojik hastalıklarda kullanılan ilaçlardandır. Tedavi aralı- ğının darlığı ve oldukça geniş yan etkisinden dolayı bu ilaçların serum düzeylerinin doğru ölçümleri önemlidir. Laboratuvarda veya laboratuvara numuneleri teslim sürecinde teknik sorunlar nedeniyle numunelerin analizi gecikebilmektedir. Sorun aynı gün çözülemiyorsa, buzdolabında bekleme süresi uzayabilir. Numunelerin uzun süreli ve uygunsuz sıcaklıkta depolanmasının yanlış sonuçlara neden olabileceği bilinmektedir. Bu çalışmada, depolama sıcaklığı ve süresinin lityum ve total valproik asit düzeylerinin, stabilitesine etkisinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Çalışma için lityum ve valproik asit kullanan 15'er hastadan alınan numuneler üçe ayrıldı. Birinci numuneler, hemen çalışıldıktan sonra oda sıcaklığında (24°C -26°C) 24 saat bekletilip tekrar çalışıldı. Hemen çalışılan örnekler bazal değer olarak kabul edildi. İkinci numuneler, +4°C'de saklandı 24 ve 48 saat sonra çalışıldı. Üçüncü numuneler ise -20°C'de 1 ay bekletildikten sonra çalışıldı. Çalışma enzimle birleştirilmiş immunoassay tekniği (EMIT) ile çalışıldı. Sonuçlar Wilcoxon testi ile karşı- laştırıldı. Bulgular: Lityum düzeylerinde hemen çalışılan ve -20°C'de 1 ay bekletildikten sonra çalışılan örnekler arasında (p=0.003) (değişim değeri %14.28); hemen çalışılan ve oda sıcaklığında (24°C -26°C) 24 saat bekletilip çalışılan örnekler arasında (p=0.001) (değişim değeri %47.14) istatistiksel olarak anlamlı fark saptandı. Diğer sonuçlarda ise anlamlı bir farklılık gözlenmedi. Valproik asit düzeylerinde de aynı şekilde; hemen çalışılan ve -20°C'de 1 ay bekletildikten sonra çalışılan örnekler arasında (p=0.015) (değişim değeri %15.78) istatistiksel olarak anlamlı fark saptandı. Diğer sonuçlarda anlamlı bir farklılık yok idi. Sonuç: Lityum içeren serum örnekleri hemen çalışılamayacağı zaman +4°C'de 2 güne kadar saklanabilir. Ancak örnekler oda sıcaklığında bekletilmemeli veya dondurulmamalıdır. Valproik asit örnekleri ise oda sıcaklığında 1 gün bekletilebilir veya +4°C'de 2 güne kadar saklanabilir, ancak örnekler dondurulmamalıdırÖğe Determining the effect of an electromagnetic field generated by a high voltage power line on rat spermatogonia cells(Dicle Üniversitesi Tıp Fakültesi, 2018) Yavaş, Mehmet Cihan; Akpolat, Veysi; Deveci, Engin; Bilgin, Hakkı Murat; Kaplan, İbrahim; Şeker, Uğur; Yıldız, İsmail; Alkış, Mehmet Eşref; Çelik, Mustafa Salih; Akdağ, Mehmet ZülküfAbstract:Objective: Purpose of the study was to research the effect of an electromagnetic field created by a high voltage line onthe testes tissue and the serum biochemistry of Wistar albino male rats. At the same time, in the study also examinedthe protective effects of melatonin and ganoderma lucidum.Methods: In the study, 64 rats were divided into eight equal groups (n: 8). In experiments lasting 26 and 52 days, thefollowing groups were used: High voltage (HV), HV + Ganoderma lucidum (GI), HV + Melatonin (MEL), and Shamcontrol.MEL (10 mg/kg) was administered intraperitoneally and GI (20 mg/kg) as oral lavage. Extremely lowfrequency electric (80.3 V/m) and magnetic fields (2.48 µT) were applied for eight hours per day. Results: The biochemical results of the study show an increase in total oxidant status (TOS) and oxidative stress index(OSI) level and a decrease in total antioxidant status (TAS) level in the 26 and 52 day high voltage groups, comparedto the control group. In the high voltage groups, the nitric oxide (NO) value increased with the increase of theexposure period. Testicular mass (p>0.05), TAS, TOS, OSI and NO (p<0.05) were found at 26 days of comparison.Testicular weight, TAS, TOS, OSI (p<0.05), and NO (p>0.05) were found in 52 day comparisons. In routinehistopathology data, we detected effects on both the 26 and 52 day HV groups, such as degeneration in spermatic cellsand full structural deterioration and increasing hyalinisation. We also detected an step up in the ligament cell in thetubular field for the 52-day test groups. In melatonin added group, proliferating cell nuclear antigen (PCNA)-positivecells were more active. TUNEL assay analysis showed that there was no significant difference between the the 26 dayexperiment groups (p>0.05), but that there was a significant dissimilarity between the 52 day experiment groups(p<0.05).Conclusions: In our study, the oxidative effect of created by a high voltage results in changes in serum biochemistry ofrats and a number of degenerative deterioration in the histological structure of testes. Aslo, it is shown that theoxidative effect created by a high voltage has negative effects on the creation of spermatogonia. GI partly protectsagainst these effects, while melatonin is more effective in this regard.Öğe Evaluation of the effect of intraperitoneal etanercept administration on oxidative stress and inflammation indicators in the kidney and blood of experimental sepsis-induced rats(Sociedade Brasileira de Medicina Tropical, 2020) Aydın, Emre E.; Yıldırım, Yaşar; Aydın, Fatma Yılmaz; Bahadır, Mehmet Veysi; Kaplan, İbrahim; Kadiroğlu, Berfin; Ketani, Muzaffer Aydın; Yılmaz, Zülfükar; Kadiroǧlu, Ali Kemal; Yılmaz, Mehmet EminIntroduction: Sepsis is an important cause of mortality and morbidity, and inflammatory response and oxidative stress play major roles underlying its pathophysiology. Here, we evaluated the effect of intraperitoneal etanercept administration on oxidative stress and inflammation indicators in the kidney and blood of experimental sepsis-induced rats. Methods: Twenty-eight adult Sprague Dawley rats were classified into Control (Group 1), Sepsis (Group 2), Sepsis+Cefazolin (Group 3), and Sepsis+Cefazolin+Etanercept (Group 4) groups. Kidney tissue and serum samples were obtained for biochemical and histopathological investigations and examined for the C reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), triggering receptor expressed on myeloid cells (TREM), and malondialdehyde (MDA) levels. Results: The levels of TNF-α, TREM, and MDA in serum and kidney samples were significantly higher in rats from sepsis group than in rats from control group (p < 0.05). Group 3 showed a significant reduction in serum levels of TNF-α, CRP, and TREM as compared with Group 2 (p < 0.05). Serum TNF-α, CRP, TREM, and MDA levels and kidney TNF-α and TREM levels were significantly lower in Group 4 than in Group 2 (p < 0.05). Serum TNF-α and TREM levels in Group 4 were significantly lower than those in Group 3, and histopathological scores were significantly lower in Group 3 and Group 4 than in Group 2 (p < 0.05). Histopathological scores of Group 4 were significantly lower than those of Group 3 (p < 0.05). Conclusions: Etanercept, a TNF-α inhibitor, may ameliorate sepsis-induced oxidative stress, inflammation, and histopathological damage.Öğe Gene polymorphisms of adducin GLY460TRP, ACE I/D, and AGT M235T in pediatric hypertension patients(International Scientific Literature Inc., 2014) Kaplan, İbrahim; Sancaktar, Enver; Ece, Aydın; Şen, Velat; Tekkeşin, Nilgün; Başaralı, Mustafa Kemal; Kelekçi, Selvi; Evliyaoğlu, OsmanBackground: Hypertension is a major global public health problem that affects both pediatric and adult populations. ACE I/D, AGT M235T, and ADD Gly460Trp polymorphisms are thought to be associated with primary hypertension. In the present study, we examined the frequency of these polymorphisms in a pediatric population with secondary hypertension. Material/Methods: Included in the study were 58 hypertensive and 58 normotensive pediatric patients. ACE I/D and AGT M235T polymorphisms are determined by conventional PCR; ADD Gly460Trp polymorphism was investigated using PCR amplification of genomic DNA. Results: There were significant differences between the control group and pediatric hypertensive group in terms of ACE I/D (P<0.05) and AGT M235T (P[removed]0.05) polymorphism. Conclusions: We suggest that RAS gene polymorphisms (ACE-I/D, AGT M235T) are significantly associated with susceptibility to diseases that lead to secondary hypertension.Öğe İdrar kültürü testi gerekliliğini öngörmede tam otomatik idrar analizi sonuçlarının performansı(Modestum Publishing Ltd., 2014) Yüksel, Hatice; Kaplan, İbrahim; Dal, Tuba; Kuş, Seyit; Toprak, Gülten; Evliyaoğlu, OsmanAmaç: Tam idrar analizi ve idrar kültürü idrar yolu enfeksiyonlarının tanısı için sıklıkla yapılan incelemelerdendir. Çalışmamızın amacı tam idrar analizinin tanısal performansını ve idrar kültürü gerekliliğini belirlemedeki rolünü incelemektir.Yöntemler: İdrar kültürü ve tam idrar analizi sonucu olan 362 hasta sonucu retrospektif olarak incelendi. Kültür sonuçları referans kabul edilerek, idrarın kimyasal ve mikroskopik incelemesinde, idrar yolu enfeksiyonu göstergesi olabilecek testlerin tanısal doğruluk parametreleri hesaplandı ve kültür istemini öngörmedeki performansı değerlendirildi.Bulgular: Toplam 362 hastaya ait idrar kültürü sonuçlarının %67 si negatifti ve bu kültür sonucu negatif olan örneklerin %50,4 ünde kimyasal analizde lökosit esteraz, nitrit ve mikroskopik analizde lökosit ve bakteri sonuçları normal olarak bulundu. Tanısal doğruluk hesaplamalarında, lökosit esteraz (%86,1) ve mikroskopide lökosit (%88,0) testlerinin sensitiviteleri yüksek bulunurken, nitrit (%95,4) ve bakteri (%86,6) incelemelerinin spesifiteleri yüksek bulundu. Mikroskopide lökosit incelemesi için yapılan ROC analizinde eğri altındaki alan 0,852 olarak hesaplandı. Sonuç: Tam otomatik idrar cihazları, tam idrar analizi için yeterli tanısal doğruluğu sağlayabilmektedir. Tam idrar analizi sonuçlarının etkin bir şeklide değerlendirilmesi kültür istemleri için gerekliliği öngörebilir ve laboratuvarlarda özellikle iş yükü ve maliyetin azalmasına katkı sağlayabilir.Öğe Investigation of The Effects of Electromagnetic Fields on SerumParaoxonase (PON1)(2021) Kaplan, İbrahim; Çelik, Mustafa Salih; Yavaş, Mehmet Cihan; Akdağ, Mehmet ZülküfINTRODUCTION: The widespread use of electrical devices inthe society and being in close contact with these devices causeboth anxiety and affect public health. The aim of this study is toinvestigate whether high voltage induced electromagnetic fieldexposure on different days affects serum paraoxanase 1activity of rats. METHODS: The study included 24 adult male wistar albinorats, weighing 320 grams on average. They were randomlygrouped into 3 as n: 8. Group 1: Sham-control, Group 2: 26days High voltage (HV) and Group 3: 52 days HV. The rats inthe 26-day and 52-day experimental groups (Groups I, II)received electric and magnetic fields for 8 hours each day. RESULTS: We found a decrease in paraoxonase (PON1)activity in the 26- and 52-day high-voltage exposure groupcompared to the control group. A statistically significant(p=0.000) change was found between the groups. DISCUSSION AND CONCLUSION: :Our study suggests thatthe exposure to high voltage line may cause a reduction ofparaoxonase 1 activity. We've noticed that short-termexposures, compared to long-term ones, may lead to lowerparaoxonase 1 activity. Therefore, our study also points to theimportance of further research in this regard.Öğe İskemik inmenin akut ve subakut döneminde matriks metalloproteinaz-2 ve matriks metalloproteinaz-9 düzeylerinin değerlendirilmesi(Dicle Üniversitesi Tıp Fakültesi, 2016) Varol, Sefer; Çevik, Mehmet Uğur; Acar, Abdullah; Demirpençe, Özlem; Kaplan, İbrahim; Taşdemir, Nebahat; Özdemir, Hasan HüseyinAmaç: Ateroskleroz ve inflamatuar mekanizmalar serebral iskemi patogenezinde önemli bir rol oynamaktadırlar. Matriks metalloproteinazların (MMP) aktivitesi inflamatuar hücrelerin infiltrasyonu, düz kas hücre migrasyonu ve proliferasyonu gibi aterosklerotik plak oluşumu ile ilişkili birçok süreçte gereklidir. MMP-2 ve MMP-9 serum düzeylerinin akut iskemik inmede inme şiddeti ve prognoz açısından önemini araştıran az çalışma vardır. Bu çalışmada iskemik inmeli hastaların akut ve subakut dönemdeki MMP-2 ve MMP-9 seviyeleri ve Glasgow Koma Skalası (GKS) ile bu biyokimyasal parametrelerin ilişkisi değerlendirildi. Yöntemler: Çalışmaya prospektif olarak akut dönem başvuran iskemik inmeli 64 hasta dahil edildi. İskemik inmeli bu hastaların akut ve subakut dönemlerinde serum MMP2 ve MMP-9 antikor düzeyi ELİSA yöntemi ile ölçüldü. Bulgular: Akut dönemde serum MMP-2 ortalama değerleri 4,28 ±3,36 ng/mL, subakut dönemde ise 2,27 ± 2,62 ng/mL idi (p˂0,05). Ayrıca akut dönemde MMP-9 ortalama değeri 2,83 ± 3,83 ng/mL subakut dönemde 2,27 ± 1,57 ng/mL idi (p˃0,05). MMP-2 ve MMP-9 seviyeleri ile hastaların GKS arasında korelasyon saptanmadı. Sonuç: Bu sonuçlar MMP-2 düzeyi iskemik inmeli hastaların akut döneminde subakut döneme göre yüksek olduğunu gösterir. Bu nedenle MMP-2 düzeyi iskemik inmenin patogenezinde akut dönemde rolünü destekleyebilir.Öğe Lipid panel with Non-HDL cholesterol and total cholesterol/HDL cholesterol ratio(Bolu Abant İzzet Baysal Üniversitesi, 2015) Yüksel, Hatice; Kaplan, İbrahim; Celepkolu, Tahsin; Toprak, Gülten; Aydeniz, Nurefşan; Etik, Emel; Çolpan, LeylaOBJECTIVE: In our study, the importance of the computational tests such as non-HDL cholesterol and Total cholesterol to HDL ratio for evaluating blood lipid levels were investigated.METHODS: The measured and calculated lipid parameters of 683 patients whose test inputs were done from Family Medicine Clinic were evaluated. The number of the patients were determined who had high Non-HDL cholesterol and total cholesterol / HDL cholesterol however the the routine lipid parameters were in the acceptable range.RESULTS: According to our results, 45 6.6% patients had high non-HDL cholesterol values and 127 %18,6 patients had high TC / HDL-cholesterol levels. Also, there were 11 1.6% patients with high non-HDL-cholesterol levels, and 39 %5,7 patients with high TC / HDL cholesterol ratio, while all routine lipid parameters were in acceptable limits. CONCLUSION: The addition of Non-HDL cholesterol and TC / HDL-cholesterol ratio to routine laboratory results would be beneficial because it can create an alert to clinicians in terms of lipid disorders.Öğe Lipid panelinde Non -HDL kolesterol ve Total kolesterol / HDL kolesterol oranı(2015) Aydeniz, Nurefşan; Etik, Emel; Yüksel, Hatice Gülru; Çelepkolu, Tahsin; Toprak, Gülten; Çolpan, Leyla; Kaplan, İbrahimAmaç: Çalışmamızda, kan lipid düzeylerinindeğerlendirilmesinde, hesaplamalı test olan Non -HDLkolesterol ve Total kolesterol/HDL kolesteroldeğerlendirilmesinin önemi incelenmiştir.Yöntem : Aile hekimliği polikliniğinden yapılan laboratuvaristemlerinden 683 hastanın hem ölçülen hem dehesaplanan lipid parametreleri değerlendirildi. Rutindeçalışılan lipid parametrelerine göre kabul edilebilir aralıktaolup da Non -HDL kolesterol ve To tal kolesterol/HDLdeğerine göre hedef değerlerin üstünde kalan hastasayıları belirlendi.Bulgular: Çalışmamızın sonuçlarına göre, 45 (%6,6) hastayüksek Non -HDL kolesterol değerlerine ve 127 (%18,6)hasta yüksek TK/HDL -K oranına sahiptir. Tüm rutin lipidparametreleri kabul edilebilir sınırlarda iken, Non -HDLkolesterol değerleri yüksek olan 11 (%1,6) hasta ve veTK/HDL kolesterol oranı yüksek olan 39 (%5,7) hastasaptandı. saptandı.Sonuç : Non-HDL kolesterol değerinin ve TK/HDL -Koranının rutin lipit son uçlarına eklenmesi, klinisyenlerelipit bozukluğu açısından uyarı oluşturabileceğindenfaydalı olacaktır.Öğe Major depresif bozuklukta prolidaz aktivitesi(Modestum Publishing Ltd., 2015) Demir, Süleyman; İbiloğlu, Aslıhan Okan; Güneş, Mehmet; Bulut, Mahmut; Atlı, Abdullah; Kaya, Mehmet Cemal; Kaplan, İbrahim; Sır, AytekinAmaç: Prolidaz enzimi dipeptitlerin karboksil terminal pozisyondaki prolin veya hidroksiprolini ayıran plazma, beyin ve çeşitli organlarda bulunan bir egzopeptidazdır. Biz bu çalışmada major depresif bozukluk (MDB)’da prolidaz aktivitesini araştırmayı amaçladık. Yöntemler: Çalışmamızda hasta grubu major depresif bozukluk 22 kişiden oluşurken kontrol grubu ise herhangi bir psikiyatrik rahatsızlığı olmayan sağlıklı 26 kişiden oluşmaktaydı. Her hasta deneyimli psikiyatristler tarafından ayrıntılı tanısal değerlendirmeye alındı. Hasta ve kontrol grubuna sosyodemografik bilgi formu verilirken, hasta grubuna Hamilton Depresyon Derecelendirme Ölçeği (HDDÖ), Hamilton Anksiyete Derecelendirme Ölçeği (HADÖ), Klinik Global İzlem Ölçeği (KGİ) uygulandı. Biyokimyasal analizler için kan alındı. Bulgular: Hasta grubunun yaş ortalaması 31,3±10,1 yıl, kontrol grubunun yaş ortalaması ise 32,3±8,8 yıl idi. Eğitim süresi hasta grubunun 8,1±6,2 yıl, kontrol grubunda 10,2±3,8 yıl idi. Gruplar arasında yaş ve eğitim süresi açısından istatistiksel olarak anlamlı farklılık belirlenmedi (p>0,05). Prolidaz aktivitesinin hasta grubunda 510.3±480.8 U/L iken kontrol grubunda 457,8±386,0 U/L olduğu görüldü. Prolidaz aktivitesi bakımında hasta ve kontrol grubu arasında anlamlı farklılık gözlenmedi (p>0,05). Sonuç: Çalışmamızda prolidaz aktivitesi MDB’ta sağlıklı kişilerle benzer bulunmuştur. Bu verinin MDB ve bipolar depresyonun farklı klinik antiteler olduğunu biyokimyasal düzeyde gösteren bir bulgu olabileceğini önermekteyiz.Öğe The measurement of neutrophil gelatinase associated lipocalin in umbilical cord blood and the assessment of its relationship with neonatal results(Dicle Üniversitesi Tıp Fakültesi, 2022) İlter, Seçkin; Ertuğrul, Sabahattin; Değer, İbrahim; Kaplan, İbrahimObjectives: In this study, the relationship of cord blood Neutrophil Gelatinase-Associated Lipocalin (NGAL) with neonatal diseases was investigated. Methods: NGAL levels were measured in the cord blood of 180 babies born between 2015 and 2016. Patients were classified according to maternal diseases, neonatal diseases and demographic characteristics. Obtained data were compared with cord blood NGAL levels. Results: In our study, the mean NGAL levels were 1283.99 ng/mL in boys and 1306.52 ng/mL in girls. Umbilical cord blood NGAL levels of infants diagnosed with intrauterine growth retardation (1913.4±2833.5 ng/mL) and prolonged premature rupture of membranes (2594.2±2037.1 ng/mL) were found to be statistically high (p<0.05). There was no statistically significant difference between NGAL levels in infants of mothers with gestational diabetes mellitus, acyanotic congenital heart diseases, meconium aspiration syndrome, infants of mothers with preeclampsia, Apgar scores and infants of mothers with oligohydramnios (p>0.05). Conclusions: Neutrophil Gelatinase-Associated Lipocalin, may be useful as a diagnostic biomarker in the evaluation of maternal and neonatal diseases. However, studies on larger patient populations are needed.Öğe Prognostic value of Golgi P 73 and Beta 2-microglobulin in patients with Viral Hepatitis B(Acta Biochimica Polonica, 2020) Özcan, Deveci; Kaplan, İbrahim; Tekİn, Recep; Çelen, Mustafa Kemal; Bozkurt, Fatma; Aslan, Emel; Cerrah, SerkanPrevious studies detected higher Golgi protein 73 levels in the serum of patients with chronic liver disease. The Beta-2 microglobulin levels were also observed to be higher in patients with chronic hepatitis B infection compared to the inactive carriers and the protein plays an important role in the response to viral infections. The aim of the present study was to assess the liver fibrosis through non-invasive methods in chronic hepatitis B patients. Three groups were included in the study. The first group comprised of the patients who were admitted to the Infectious Diseases and Clinical Microbiology clinic to undergo a liver biopsy, while the second group included the patients who were admitted inactive hepatitis B carriers. The third group comprised the healthy controls. The Golgi p-73 and Beta-2 microglobulin levels in the plasma were determined using the ELISA method. Beta-2 microglobulin level was highest in the patients group and the difference was statistically significant. No significant difference was observed between the carriers group and the group of healthy controls. The Golgi P-73 values were significantly higher in the patients group in comparison to both other groups. However, the mean Golgi p-73 value was also significantly higher in the carrier group compared to the control group. In patients who are followed up with the diagnosis of chronic hepatitis B and who have undergone biopsies as candidates for treatment, the Beta-2 microglobulin and Golgi p-73 values may be important markers since they indicate the extent of the liver damage.Öğe Prophylactic administration of silybin ameliorates L-arginine-induced acute pancreatitis(International Scientific Literature Inc., 2016) Uçmak, Feyzullah; Ekin, Nazım; İbiloğlu, İbrahim; Arslan, Serkan; Kaplan, İbrahim; Şenateş, EbubekirBackground: Oxidative stress have been shown to play a role in the pathogenesis of acute pancreatitis. The aim of this study was to investigate the potential effect of silybin, a potent antioxidant, on L-arginine-induced acute pancreatitis in an experimental rat model. Material/Methods: Forty female Wistar Albino rats were divided into 5 groups as follows: Group 1 (C): control group (n=8), Group 2 (SL): silybin group (n=8), Group 3 (LA): acute pancreatitis group (n=8), Group 4 (SLLA): prophylaxis group (n=8), and Group 5 (LASL): treatment group (n=8). Group C (control) received 2 intraperitoneal (i.p.) injections of physiological saline at an interval of 1 h. Group SL received only a single i.p. injection of silybin. The SLLA group received a single i.p. injection of silybin before the induction of acute pancreatitis with L-arginine, whereas the LASL group received the same injection after the induction of acute pancreatitis with L-arginine. Pancreatic tissues were histopathologically examined. Levels of amylase and oxidative stress markers (total oxidant status and total anti-oxidant status) were determined in the blood samples. Oxidative stress index was calculated. Results: In comparison to the LA, the prophylaxis and treatment groups showed significant improvements in serum oxidative stress parameters (p=0.001 and p=0.005, respectively). Histopathological analysis showed that the treatment group had significant improvements in edema scores only (p=0.006), whereas the prophylaxis group had the same improvements in inflammation and necrosis scores as well as in total scores (p=0.004, 0.006, and 0.004, respectively). Conclusions: When used for prophylactic rather than therapeutic purposes, silybin ameliorates serum oxidative stress parameters and improves histopathological results via its antioxidant and anti-inflammatory properties.
