Kinetic approach for the purification of nucleotides with magnetic separation

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dc.contributor.authorTural, Servet
dc.contributor.authorTural, Bilsen
dc.contributor.authorEce, Mehmet Şakir
dc.contributor.authorYetkin, Evren
dc.contributor.authorÖzkan, Necati
dc.contributor.orcid0000-0003-2479-9438
dc.contributor.orcid0000-0001-7555-2481
dc.contributor.orcid0000-0002-9411-314X
dc.contributor.orcid0000-0002-7837-3152
dc.date.accessioned2024-04-24T15:59:27Z
dc.date.available2024-04-24T15:59:27Z
dc.date.issued2014
dc.departmentDicle Üniversitesi, Ziya Gökalp Eğitim Fakültesi, Matematik ve Fen Bilimleri Eğitimi Bölümüen_US
dc.description.abstractThe isolation of beta-nicotinamide adenine dinucleotide is of great importance since it is widely used in different scientific and technologic fields such as biofuel cells, sensor technology, and hydrogen production. In order to isolate beta-nicotinamide adenine dinucleotide, first 3-aminophenyboronic acid functionalized magnetic nanoparticles were prepared to serve as a magnetic solid support and subsequently they were used for reversible adsorption/desorption of beta-nicotinamide adenine dinucleotide in a batch fashion. The loading capacity of the 3-aminophenyboronic acid functionalized nanoparticles for beta-nicotinamide adenine dinucleotide adsorption was 13.0 mu mol/g. Adsorption kinetic and isotherm studies showed that the adsorption process followed a pseudo-second-order kinetic model and the experimental data can be represented using Langmuir isotherm model. The 3-aminophenyboronic acid functionalized magnetic nanoparticles were proposed as an alternative support for the beta-nicotinamide adenine dinucleotide purification. The results elucidated the significance of magnetic separation as a fast, relatively simple, and low-cost technique. Furthermore, the magnetic supports can be reused at least five times for purification processes.en_US
dc.description.sponsorshipDicle University Research Fund (DUBAP) [13-ZEF-28, 12-ZEF-110, 10-ZEF-2 8]en_US
dc.description.sponsorshipThis project is funded by the financial support from Dicle University Research Fund (DUBAP, Project No. 13-ZEF-28, 12-ZEF-110 and 10-ZEF-2 8).en_US
dc.identifier.citationTural, S., Tural, B., Ece, M. Ş., Yetkin, E. ve Özkan, N. (2014). Kinetic approach for the purification of nucleotides with magnetic separation. Journal of Separation Science, 37(22), 3370-3376.
dc.identifier.doi10.1002/jssc.201400648
dc.identifier.endpage3376en_US
dc.identifier.issn1615-9306
dc.identifier.issn1615-9314
dc.identifier.issue22en_US
dc.identifier.pmid25199632
dc.identifier.scopus2-s2.0-84910012015
dc.identifier.scopusqualityQ2
dc.identifier.startpage3370en_US
dc.identifier.urihttps://doi.org/10.1002/jssc.201400648
dc.identifier.urihttps://hdl.handle.net/11468/14081
dc.identifier.urihttps://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.201400648
dc.identifier.volume37en_US
dc.identifier.wosWOS:000344799900020
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTural, Servet
dc.institutionauthorTural, Bilsen
dc.institutionauthorEce, Mehmet Şakir
dc.institutionauthorYetkin, Evren
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofJournal of Separation Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAdsorptionen_US
dc.subjectDesorptionen_US
dc.subjectMagnetic assisted separationen_US
dc.subjectNucleotidesen_US
dc.subjectReusabilityen_US
dc.titleKinetic approach for the purification of nucleotides with magnetic separationen_US
dc.titleKinetic approach for the purification of nucleotides with magnetic separation
dc.typeArticleen_US

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