Kinetic approach for the purification of nucleotides with magnetic separation
Yükleniyor...
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The isolation of beta-nicotinamide adenine dinucleotide is of great importance since it is widely used in different scientific and technologic fields such as biofuel cells, sensor technology, and hydrogen production. In order to isolate beta-nicotinamide adenine dinucleotide, first 3-aminophenyboronic acid functionalized magnetic nanoparticles were prepared to serve as a magnetic solid support and subsequently they were used for reversible adsorption/desorption of beta-nicotinamide adenine dinucleotide in a batch fashion. The loading capacity of the 3-aminophenyboronic acid functionalized nanoparticles for beta-nicotinamide adenine dinucleotide adsorption was 13.0 mu mol/g. Adsorption kinetic and isotherm studies showed that the adsorption process followed a pseudo-second-order kinetic model and the experimental data can be represented using Langmuir isotherm model. The 3-aminophenyboronic acid functionalized magnetic nanoparticles were proposed as an alternative support for the beta-nicotinamide adenine dinucleotide purification. The results elucidated the significance of magnetic separation as a fast, relatively simple, and low-cost technique. Furthermore, the magnetic supports can be reused at least five times for purification processes.
Açıklama
Anahtar Kelimeler
Adsorption, Desorption, Magnetic assisted separation, Nucleotides, Reusability
Kaynak
Journal of Separation Science
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
37
Sayı
22
Künye
Tural, S., Tural, B., Ece, M. Ş., Yetkin, E. ve Özkan, N. (2014). Kinetic approach for the purification of nucleotides with magnetic separation. Journal of Separation Science, 37(22), 3370-3376.
