Expression of GRP78 and its copartners in HEK293 and pancreatic cancer cell lines (BxPC-3/PANC-1) exposed to MRI and CT contrast agents

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dc.authorid0000-0001-5518-5004en_US
dc.authorid0000-0003-4637-0253en_US
dc.authorid0000-0001-5311-8887en_US
dc.authorid0000-0002-4363-5861en_US
dc.contributor.authorAzzawri, Ali Ahmed
dc.contributor.authorYıldırım, İbrahim Halil
dc.contributor.authorYeğin, Zeynep
dc.contributor.authorDuşak, Abdürrahim
dc.date.accessioned2023-10-19T08:30:43Z
dc.date.available2023-10-19T08:30:43Z
dc.date.issued2023en_US
dc.departmentDicle Üniversitesi, Veteriner Fakültesi, Zootekni ve Hayvan Besleme Bölümü, Veterinerlik Genetiği Anabilim Dalıen_US
dc.description.abstractEndoplasmic reticulum (ER) stress-associated chaperones trigger a defense mechanism called as unfolded protein response (UPR) which can manage apoptosis and be determinative in cell fate. Both anticancer drug effects and potential toxicity effects of magnetic resonance imaging (MRI) and computed tomography (CT) contrast agents were aimed to be evaluated. For this purpose, we investigated expression profiles of endoplasmic reticulum stress-associated chaperone molecules in human pancreatic tumor lines BxPC-3 and PANC-1 and control human embryonic kidney cells 293 (HEK293) induced with a variety of gadolinium and iohexol contrast agents. Protein expression levels of ER stress-associated chaperones (master regulator: GRP78/Bip and its copartners: Calnexin, Ero1, PDI, CHOP, IRE1α and PERK) were evaluated with Western blotting. Expression levels at mRNA level were also assessed for GRP78/Bip and CHOP with real-time PCR. Induction of cells was carried out with four different Gd-based contrast agents (GBCAs): (Dotarem, Optimark, Primovist and Gadovist) and two different iohexol agents (Omnipol, Omnipaque). CT contrast agents tested in the study did not result in significant ER stress in HEK293 cells. However, they do not seem to have theranostic potential in pancreas cancer through ER pathway. The potential efficiency of macrocyclic MRI contrast agents to provoke apoptosis via ER stress-associated chaperones in BxPC-3 cells lends credibility for their future theranostic use in pancreas cancer as long as undesired toxicity effects were carefully considered. ER stress markers and/or contrast agents seem to have promising potential to be translated into the clinical practice to manage pancreas cancer progression.en_US
dc.identifier.citationAzzawri, A. A., Yıldırım, İ. H., Yeğin, Z. ve Duşak, A. (2023). Expression of GRP78 and its copartners in HEK293 and pancreatic cancer cell lines (BxPC-3/PANC-1) exposed to MRI and CT contrast agents. Nucleosides, Nucleotides and Nucleic Acids, Article in Press.en_US
dc.identifier.doi10.1080/15257770.2023.2263496
dc.identifier.endpage26en_US
dc.identifier.issn1525-7770
dc.identifier.pmid37787049
dc.identifier.scopus2-s2.0-85173550564
dc.identifier.scopusqualityQ4
dc.identifier.startpage1en_US
dc.identifier.urihttps://www.tandfonline.com/doi/full/10.1080/15257770.2023.2263496
dc.identifier.urihttps://hdl.handle.net/11468/12875
dc.identifier.volumeArticle in Press
dc.identifier.wosWOS:001075541200001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorYıldırım, İbrahim Halil
dc.language.isoenen_US
dc.publisherTaylor and Francis Ltd.en_US
dc.relation.ispartofNucleosides, Nucleotides and Nucleic Acids
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEndoplasmic reticulum stressen_US
dc.subjectGadoliniumen_US
dc.subjectGRP78/bipen_US
dc.subjectIohexolen_US
dc.subjectPancreatic canceren_US
dc.subjectUnfolded protein responseen_US
dc.titleExpression of GRP78 and its copartners in HEK293 and pancreatic cancer cell lines (BxPC-3/PANC-1) exposed to MRI and CT contrast agentsen_US
dc.titleExpression of GRP78 and its copartners in HEK293 and pancreatic cancer cell lines (BxPC-3/PANC-1) exposed to MRI and CT contrast agents
dc.typeArticleen_US

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