Pregnancy-associated glycoproteins concentrations during early gestation in pregnant Awassi sheep

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dc.authorid0000-0003-0684-7213en_US
dc.contributor.authorAkköse, Mehmet
dc.contributor.authorÇınar, Elif Merve
dc.contributor.authorYazlık, Murat Onur
dc.contributor.authorÇebi-Şen, Çiğdem
dc.contributor.authorPolat, Yadigar
dc.date.accessioned2021-10-01T08:06:55Z
dc.date.available2021-10-01T08:06:55Z
dc.date.issued2021en_US
dc.departmentDicle Üniversitesi, Veteriner Fakültesi, Klinik Bilimler Bölümü, Dölerme ve Suni Tohumlama Ana Bilim Dalıen_US
dc.descriptionWOS:000697695600007
dc.description.abstractThis study was aimed at determining the serum pregnancy-associated glycoproteins (PAG) profile of pregnant Awassi sheep during early gestation. A total of 122 Awassi ewes were used, which were in days 28-56 (28, 30, 32, 36, 42, 49, and 56) of gestation. Blood samples were taken via jugular venepuncture. Out of these animals, 104 ewes that had delivered (singleton lambs, n = 60; twin lambs, n = 44), according to lambing records, were selected for the assessment of serum PAG levels. The serum PAG levels was measured using a commercial enzyme-linked immunosorbent assay (ELISA) kit (IDEXX Bovine Pregnancy Test). Whereas serum PAG levels (mean ± standard error) on days 28, 30, 32, 36 and 42 of gestation were found to be similar (0.237 ± 0.031, 0.402 ± 0.067, 0.329 ± 0.036, 0.397 ± 0.075, and 0.409 ± 0.060, respectively), the levels measured on days 49 and 56 of gestation were significantly higher (0.712 ± 0.088 and 0.798 ± 0.115, respectively) (p < 0.05). The serum PAG levels of the twin-pregnant ewes on days 28, 30 and 42 of gestation were higher than those of the singlepregnant ewes (twin-pregnant ewes: 0.314, 0.648 and 0.594, respectively; single-pregnant ewes: 0.186, 0.258 and 0.308, respectively; p < 0.05). With a cut-off sample minus negative (S–N) value set at > 0.300, the sensitivity of serum PAG level-based pregnancy diagnosis was 51.95% between days 28 and 42 of gestation and 92.69% between days 49 and 56 of gestation. On the other hand, with a cut-off S–N value set at > 0.100, the sensitivity of the PAG ELISA test was 94.81% between days 28 and 42 of gestation and 100% between days 49 and 56 of gestation. In conclusion, the serum PAG levels of the pregnant Awassi sheep remained similar between days 28 and 42 of gestation, but progressively increased thereafter. The serum PAG levels of the twin-pregnant Awassi ewes were significantly higher than those of the single-pregnant sheep on days 28, 30 and 42 of gestation, but they were not different on days 32, 36, 49 and 56 of gestation.en_US
dc.identifier.citationAkköse, M., Çınar, E.M., Yazlık, M.O., Çebi-Şen, Ç. ve Polat, Y. (2021). Pregnancy-associated glycoproteins concentrations during early gestation in pregnant Awassi sheep. Medycna Weterynary JNA-Veterinary Medicine-Science and Practice, 77(11), 563-567.en_US
dc.identifier.doi10.21521/mw.6564
dc.identifier.endpage567en_US
dc.identifier.issn0025-8628
dc.identifier.issue11en_US
dc.identifier.scopus2-s2.0-85117494115
dc.identifier.scopusqualityQ4
dc.identifier.startpage563en_US
dc.identifier.urihttp://www.medycynawet.edu.pl/images/stories/pdf/pdf2021/112021/2021116564.pdf
dc.identifier.urihttps://hdl.handle.net/11468/7854
dc.identifier.volume77en_US
dc.identifier.wosWOS:000697695600007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorÇınar, Elif Merve
dc.language.isoenen_US
dc.publisherPolish Soc Veterinary Sciences Editorial Officeen_US
dc.relation.ispartofMedycna Weterynary JNA-Veterinary Medicine-Science and Practice
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAwassien_US
dc.subjectPregnancyen_US
dc.subjectPregnancy-associated glycoproteinsen_US
dc.subjectSheepen_US
dc.titlePregnancy-associated glycoproteins concentrations during early gestation in pregnant Awassi sheepen_US
dc.titlePregnancy-associated glycoproteins concentrations during early gestation in pregnant Awassi sheep
dc.typeArticleen_US

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