Pregnancy-associated glycoproteins concentrations during early gestation in pregnant Awassi sheep
Yükleniyor...
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Polish Soc Veterinary Sciences Editorial Office
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
This study was aimed at determining the serum pregnancy-associated glycoproteins (PAG) profile of pregnant
Awassi sheep during early gestation. A total of 122 Awassi ewes were used, which were in days 28-56 (28, 30,
32, 36, 42, 49, and 56) of gestation. Blood samples were taken via jugular venepuncture. Out of these animals,
104 ewes that had delivered (singleton lambs, n = 60; twin lambs, n = 44), according to lambing records, were
selected for the assessment of serum PAG levels. The serum PAG levels was measured using a commercial
enzyme-linked immunosorbent assay (ELISA) kit (IDEXX Bovine Pregnancy Test). Whereas serum PAG levels
(mean ± standard error) on days 28, 30, 32, 36 and 42 of gestation were found to be similar (0.237 ± 0.031,
0.402 ± 0.067, 0.329 ± 0.036, 0.397 ± 0.075, and 0.409 ± 0.060, respectively), the levels measured on days 49 and
56 of gestation were significantly higher (0.712 ± 0.088 and 0.798 ± 0.115, respectively) (p < 0.05). The serum
PAG levels of the twin-pregnant ewes on days 28, 30 and 42 of gestation were higher than those of the singlepregnant ewes (twin-pregnant ewes: 0.314, 0.648 and 0.594, respectively; single-pregnant ewes: 0.186, 0.258 and
0.308, respectively; p < 0.05). With a cut-off sample minus negative (S–N) value set at > 0.300, the sensitivity
of serum PAG level-based pregnancy diagnosis was 51.95% between days 28 and 42 of gestation and 92.69%
between days 49 and 56 of gestation. On the other hand, with a cut-off S–N value set at > 0.100, the sensitivity
of the PAG ELISA test was 94.81% between days 28 and 42 of gestation and 100% between days 49 and 56 of
gestation. In conclusion, the serum PAG levels of the pregnant Awassi sheep remained similar between days 28
and 42 of gestation, but progressively increased thereafter. The serum PAG levels of the twin-pregnant Awassi
ewes were significantly higher than those of the single-pregnant sheep on days 28, 30 and 42 of gestation, but
they were not different on days 32, 36, 49 and 56 of gestation.
Açıklama
WOS:000697695600007
Anahtar Kelimeler
Awassi, Pregnancy, Pregnancy-associated glycoproteins, Sheep
Kaynak
Medycna Weterynary JNA-Veterinary Medicine-Science and Practice
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
77
Sayı
11
Künye
Akköse, M., Çınar, E.M., Yazlık, M.O., Çebi-Şen, Ç. ve Polat, Y. (2021). Pregnancy-associated glycoproteins concentrations during early gestation in pregnant Awassi sheep. Medycna Weterynary JNA-Veterinary Medicine-Science and Practice, 77(11), 563-567.
