Evaluation of PECAM-1 and p38 MAPK expressions in cerebellum tissue of rats treated with caffeic acid phenethyl ester: A biochemical and immunohistochemical study

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dc.authorid0000-0002-2353-1184en_US
dc.contributor.authorÇetin, Abdurrahman
dc.contributor.authorDeveci, Engin
dc.date.accessioned2021-10-26T07:09:07Z
dc.date.available2021-10-26T07:09:07Z
dc.date.issued2019en_US
dc.departmentDicle Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalıen_US
dc.descriptionPMID:30543083
dc.description.abstractBackground: This study aimed to investigate the antioxidative and anti-inflammatory effects of caffeic acid phenethyl ester (CAPE) on damage caused to cerebellum tissue by diffuse traumatic head trauma via biochemical, histopathologic, and immuno-histochemical methods. Materials and methods: Male Sprague-Dawley (300–350 g) rats were subjected to traumatic brain injury with a weight-drop device (300 g/1 m weight-height impact). Twenty-four adult rats were randomly divided into three equal groups of 8, including a control group, traumatic brain injury (TBI) group, and TBI + CAPE treatment group (10 μmoL/kg/i.p.). Cerebellum tissue samples taken from anterior lobe from all rats were taken 7 days after traumatic injury and were subjected to biochemical and histopathological analysis, as well as immunohistochemical analysis for platelet endothelial cell adhesion molecule-1 (PECAM-1) and phosphate 38-mitogen-activated protein kinase (p38 MAPK). Results: In the TBI group, the granular layer had dilatation and haemorrhage in the capillary vessels and inflammatory cell infiltration around the periphery of the blood vessels. In the TBI + CAPE group, the small capillaries in the white matter were slightly dilated, there were no inflammatory cells, and dense chromatin/ granular cells were observed in the granular layer. Also in the TBI + CAPE group, the Purkinje cells of the ganglion cell layer had ovoid nuclei, were chromatin-rich, and their extensions protruded to the molecular layer. CAPE is thought to regulate inflammation, cell damage, and angiogenetic development by affecting the PECAM-1 and p38 MAPK proteins. Conclusions: These proteins are key modulators of endothelial integrity and neuroinflammation in vessels in response to endothelial damage as well as of the proinflammatory response in the cerebellum in response to traumatic damage.en_US
dc.identifier.citationÇetin, A. ve Deveci, E. (2019). Evaluation of PECAM-1 and p38 MAPK expressions in cerebellum tissue of rats treated with caffeic acid phenethyl ester: A biochemical and immunohistochemical study. Folia Morphologica (Poland), 78(2), 221-229.en_US
dc.identifier.doi10.5603/FM.a2018.0085
dc.identifier.endpage229en_US
dc.identifier.issn0015-5659
dc.identifier.issue2en_US
dc.identifier.pmid30543083
dc.identifier.scopus2-s2.0-85067285327
dc.identifier.scopusqualityQ2
dc.identifier.startpage221en_US
dc.identifier.urihttps://journals.viamedica.pl/folia_morphologica/article/view/58795
dc.identifier.urihttps://hdl.handle.net/11468/8012
dc.identifier.volume78en_US
dc.identifier.wosWOS:000469835600001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorDeveci, Engin
dc.language.isoenen_US
dc.publisherVia Medicaen_US
dc.relation.ispartofFolia Morphologica (Poland)
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCerebellumen_US
dc.subjectP38 MAPKen_US
dc.subjectPECAM-1en_US
dc.subjectRaten_US
dc.subjectTraumatic brain injuryen_US
dc.titleEvaluation of PECAM-1 and p38 MAPK expressions in cerebellum tissue of rats treated with caffeic acid phenethyl ester: A biochemical and immunohistochemical studyen_US
dc.titleEvaluation of PECAM-1 and p38 MAPK expressions in cerebellum tissue of rats treated with caffeic acid phenethyl ester: A biochemical and immunohistochemical study
dc.typeArticleen_US

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