Production of extracellular alkaline ?-amylase by solid state fermentation with a newly isolated Bacillus sp.
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Tarih
2008
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Production of alkaline -amylase employing our laboratory isolate, Bacillus sp., under solid state fermentation, was optimized. The effect of wheat bran and lentil husk was examined. Lentil husk exhibited the highest enzyme production. The appropriate incubation time, inoculum size, moisture level, and buffer solution level were determined. Maximum yields of 216,000 and 172,800 U/g were achieved by employing lentil husk and wheat bran as substrates in 0.1 M carbonate/bicarbonate buffer at pH 10.0 with 30% initial moisture level at 24h. Inoculum size and buffer solution level were found to be 20% and 1:0.5 for two solid substrates.
Açıklama
Anahtar Kelimeler
Solid State Fermentation, Bacillus, Alkaline Alpha-Amylase, Agrocultural Wastes
Kaynak
Preparative Biochemistry & Biotechnology
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
38
Sayı
2
