Comparative analysis of antioxidant, anticholinesterase, and antibacterial activity of microbial chondroitin sulfate and commercial chondroitin sulfate

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dc.contributor.authorÜnver, Tuba
dc.contributor.authorErenler, Ayşe Şebnem
dc.contributor.authorBingül, Murat
dc.contributor.authorBoğa, Mehmet
dc.contributor.orcid0000-0002-8655-2716
dc.contributor.orcid0000-0002-1786-5022
dc.contributor.orcid0000-0002-3909-0694
dc.contributor.orcid0000-0003-4163-9962
dc.date.accessioned2024-04-24T15:59:21Z
dc.date.available2024-04-24T15:59:21Z
dc.date.issued2023
dc.departmentDicle Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümüen_US
dc.description.abstractChondroitin synthesis was performed using the recombinant Escherichia coli(C2987) strain created by transforming the plasmid pETM6-PACF-vgb, which carries the genes responsible for chondroitin synthesis, kfoA, kfoC, kfoF, and the Vitreoscilla hemoglobin gene (vgb). Then, Microbial chondroitin sulfate (MCS)'s antioxidant, anticholinesterase, and antibacterial activity were compared with commercial chondroitin sulfate (CCS). The antioxidant studies revealed that the MCS and CCS samples could be potential targets for scavenging radicals and cupric ion reduction. MCS demonstrated better antioxidant properties in the ABTS assay with the IC50 value of 0.66 mg than CCS. MCS showed 2.5-fold for DPPH and almost 5-fold for ABTS *+ (with a value of 3.85 mg/mL) better activity than the CCS. However, the compounds were not active for cholinesterase enzyme inhibitions. In the antibacterial assay, the Minimum inhibitory concentration (MIC) values of MCS against S. aureus, E. aerogenes, E. coli, P. aeruginosa, and K. pneumoniae (0.12, 0.18, 0.12, 0.18, and 0.18 g/mL, respectively) were found to be greater than that of CCS (0.42, 0.48, 0.36, 0.36, and 0.36 g/mL, respectively). This study demonstrates that MCS is a potent pharmacological agent due to its physicochemical properties, and its usability as a therapeutic-preventive agent will shed light on future studies.en_US
dc.description.sponsorshipWe would like to thank Wenqin He, Mattheos A.G. Koffas, and the entire research team who produced the pETM6-PACF plasmid and shared it without hesitation.en_US
dc.description.sponsorshipWe would like to thank Wenqin He, Mattheos A.G. Koffas, and the entire research team who produced the pETM6-PACF plasmid and shared it without hesitation.en_US
dc.identifier.citationÜnver, T., Erenler, A. Ş., Bingül, M. ve Boğa, M. (2023). Comparative analysis of antioxidant, anticholinesterase, and antibacterial activity of microbial chondroitin sulfate and commercial chondroitin sulfate. Chemistry and Biodiversity, 20(10), 1-9.
dc.identifier.doi10.1002/cbdv.202300924
dc.identifier.endpage9
dc.identifier.issn1612-1872
dc.identifier.issn1612-1880
dc.identifier.issue10
dc.identifier.pmid37615364
dc.identifier.scopus2-s2.0-85170055312
dc.identifier.scopusqualityQ3
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1002/cbdv.202300924
dc.identifier.urihttps://hdl.handle.net/11468/14005
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/10.1002/cbdv.202300924
dc.identifier.volume20
dc.identifier.wosWOS:001060373100001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBingül, Murat
dc.institutionauthorBoğa, Mehmet
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofChemistry & Biodiversity
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnimal-Originated Chondroitin Sulfateen_US
dc.subjectAntibacterial activityen_US
dc.subjectAnticholinesterase activityen_US
dc.subjectAntioxidant agenten_US
dc.subjectMicrobial chondroitin sulfateen_US
dc.titleComparative analysis of antioxidant, anticholinesterase, and antibacterial activity of microbial chondroitin sulfate and commercial chondroitin sulfateen_US
dc.titleComparative analysis of antioxidant, anticholinesterase, and antibacterial activity of microbial chondroitin sulfate and commercial chondroitin sulfate
dc.typeArticleen_US

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