Comparative analysis of antioxidant, anticholinesterase, and antibacterial activity of microbial chondroitin sulfate and commercial chondroitin sulfate
Yükleniyor...
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Chondroitin synthesis was performed using the recombinant Escherichia coli(C2987) strain created by transforming the plasmid pETM6-PACF-vgb, which carries the genes responsible for chondroitin synthesis, kfoA, kfoC, kfoF, and the Vitreoscilla hemoglobin gene (vgb). Then, Microbial chondroitin sulfate (MCS)'s antioxidant, anticholinesterase, and antibacterial activity were compared with commercial chondroitin sulfate (CCS). The antioxidant studies revealed that the MCS and CCS samples could be potential targets for scavenging radicals and cupric ion reduction. MCS demonstrated better antioxidant properties in the ABTS assay with the IC50 value of 0.66 mg than CCS. MCS showed 2.5-fold for DPPH and almost 5-fold for ABTS *+ (with a value of 3.85 mg/mL) better activity than the CCS. However, the compounds were not active for cholinesterase enzyme inhibitions. In the antibacterial assay, the Minimum inhibitory concentration (MIC) values of MCS against S. aureus, E. aerogenes, E. coli, P. aeruginosa, and K. pneumoniae (0.12, 0.18, 0.12, 0.18, and 0.18 g/mL, respectively) were found to be greater than that of CCS (0.42, 0.48, 0.36, 0.36, and 0.36 g/mL, respectively). This study demonstrates that MCS is a potent pharmacological agent due to its physicochemical properties, and its usability as a therapeutic-preventive agent will shed light on future studies.
Açıklama
Anahtar Kelimeler
Animal-Originated Chondroitin Sulfate, Antibacterial activity, Anticholinesterase activity, Antioxidant agent, Microbial chondroitin sulfate
Kaynak
Chemistry & Biodiversity
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
20
Sayı
10
Künye
Ünver, T., Erenler, A. Ş., Bingül, M. ve Boğa, M. (2023). Comparative analysis of antioxidant, anticholinesterase, and antibacterial activity of microbial chondroitin sulfate and commercial chondroitin sulfate. Chemistry and Biodiversity, 20(10), 1-9.
