Development and validation of a high-performance liquid chromatography method for determination of cefquinome concentrations in sheep plasma and its application to pharmacokinetic studies

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dc.authorid0000-0002-8674-4873en_US
dc.authorid0000-0002-9017-763Xen_US
dc.authorid0000-0001-9059-7151en_US
dc.contributor.authorÜney, Kamil
dc.contributor.authorAltan, Feray
dc.contributor.authorElmas, Muammer
dc.date.accessioned2024-04-05T13:23:24Z
dc.date.available2024-04-05T13:23:24Z
dc.date.issued2011en_US
dc.departmentDicle Üniversitesi, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümü, Farmakoloji ve Toksikoloji Ana Bilim Dalıen_US
dc.description.abstractCefquinome has a broad spectrum of antibacterial activity and was developed especially for use in animals. A simple and sensitive high-performance liquid chromatography (HPLC) method with UV-visible detection for quantification of cefquinome concentrations in sheep plasma was developed and validated. Separation of cefquinome from plasma components was achieved on a Phenomenex Gemini C 18 column (250 mm by 4.6 mm; internal diameter [i.d.], 5 μm). The mobile phase consisted of acetonitrile and 0.1% trifluoroacetic acid in water and was delivered at a rate of 0.9 ml/min. A simple and rapid sample preparation involved the addition of methanol to 200 μl of plasma to precipitate plasma proteins followed by direct injection of 50 μl of supernatant into the high-performance liquid chromatography system. The linearity range of the proposed method was 0.02 to 12 μg/ml. The intraday and interday coefficients of variation obtained from cefquinome were less than 5%, and biases ranged from -3.76% to 1.24%. Mean recovery based on low-, medium-, and high-quality control standards ranged between 92.0 and 93.9%. Plasma samples were found to be stable in various storage conditions (freeze-thaw, postpreparative, short-term, and long-term stability). The method described was found to be readily available, practicable, cheap, rapid, sensitive, precise, and accurate. It was successfully applied to the study of the pharmacokinetics of cefquinome in sheep. This method can be very useful and an alternate to performing pharmacokinetic studies in the determination of cefquinome for clinical use.en_US
dc.identifier.citationÜney, K., Altan, F. ve Elmas, M. (2011). Development and validation of a high-performance liquid chromatography method for determination of cefquinome concentrations in sheep plasma and its application to pharmacokinetic studies. Antimicrobial Agents and Chemotherapy, 55(2), 854-859.en_US
dc.identifier.doi10.1128/AAC.01126-10
dc.identifier.endpage859en_US
dc.identifier.issn1098-6596
dc.identifier.issue2en_US
dc.identifier.pmid21098247
dc.identifier.scopus2-s2.0-78751693506
dc.identifier.scopusqualityQ1
dc.identifier.startpage854en_US
dc.identifier.urihttps://journals.asm.org/doi/epub/10.1128/aac.01126-10
dc.identifier.urihttps://hdl.handle.net/11468/13864
dc.identifier.volume55en_US
dc.identifier.wosWOS:000286422500052
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAltan, Feray
dc.language.isoenen_US
dc.relation.ispartofAntimicrobial Agents and Chemotherapy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLiquid chromatographyen_US
dc.subjectPharmacokineticen_US
dc.titleDevelopment and validation of a high-performance liquid chromatography method for determination of cefquinome concentrations in sheep plasma and its application to pharmacokinetic studiesen_US
dc.titleDevelopment and validation of a high-performance liquid chromatography method for determination of cefquinome concentrations in sheep plasma and its application to pharmacokinetic studies
dc.typeArticleen_US

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