Linalool and eugenol exhibit apoptotic potential on hela and caco-2 cells through the modulation of src kinases and ADAMTS proteases while only eugenol displays anti-angiogenic features on HeLa cells
Yükleniyor...
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The cytotoxic effects of linalool and eugenol on HeLa and Caco-2 cancer cells were investigated. The expression of the Fyn, Hck, and ADAMTS genes and Bcl-2, Bax, VEGF proteins were determined through the real-time-qPCR and Western blot analysis, respectively. IC50 values of linalool on HeLa cell were 799.6, 1228, and 1239 mu g/mL after 24, 48, and 72 h, respectively, whereas those of eugenol were 388, 94.35, and 73.78 mu g/mL. IC50 values in Caco-2 cells were 887.7, 1259, and 1356 mu g/mL for linalool whereas those of eugenol were 452.7, 578.3, and 433.3 mu g/mL after 24, 48, and 72 h, respectively. As a control, Hek-293 IC50 values were 1047 and 556.7 mu g/mL for linalool and eugenol, respectively. Linalool at 1024 mu g/mL concentration increased Bax and Bcl-2 levels significantly in both cell lines (p < 0.001). However, eugenol treatment did not alter Bax levels (p > 0.05), but it decreased Bcl-2 levels significantly (p < 0.001). Additionally, 1024 mu g/mL linalool exposure increased Fyn gene expression in Caco-2 cells, which was not observed for HeLa cells. Unlike linalool, eugenol upregulated ADAMTS1 gene expression and decreased VEGF levels in HeLa cells. On the contrary, in Caco-2 cells, eugenol treatment did not affect ADAMTS1 levels, whilst linalool upregulated ADAMTS1 levels, especially at 1024 mu g/mL concentration. VEGF levels increased in Caco-2 cells following eugenol treatment. ADAMTS3 levels decreased in Caco-2 cells with exception of 1024 mu g/mL linalool treatment. These results indicated the anti-proliferative, apoptotic effects of plant-derived natural compounds. It is also revealed that eugenol might also display antiangiogenic properties on HeLa cells in addition to its apoptotic effects.
Açıklama
Anahtar Kelimeler
ADAMTS, Cellular Apoptosis, Cancer cells, Linalool, Eugenol, Src Kinase
Kaynak
Biologica
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
77
Sayı
3
Künye
Kısacam, M.A., Sakin, F., İrtegün-Kandemir, S., Pektanç-Şengül, G.ve Kürekçi, C. (2022). Linalool and eugenol exhibit apoptotic potential on hela and caco-2 cells through the modulation of src kinases and ADAMTS proteases while only eugenol displays anti-angiogenic features on HeLa cells. Biologica, 77(3), 865-877.
