Poly(Hydroxyethyl methacrylate) immunoaffinity cryogel column for the purification of human immunoglobulin M

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dc.authorid0000-0002-0761-6436en_US
dc.contributor.authorBakhshpour, Monireh
dc.contributor.authorTopçu, Aykut Arif
dc.contributor.authorBereli, Nilay
dc.contributor.authorAlkan, Huseyin
dc.contributor.authorDenizli, Adil
dc.date.accessioned2021-07-14T12:46:03Z
dc.date.available2021-07-14T12:46:03Z
dc.date.issued2020en_US
dc.departmentDicle Üniversitesi, Fen Bilimleri Enstitüsü, Kimya Ana Bilim Dalıen_US
dc.descriptionWOS:000523677000006
dc.descriptionPMID: 32013072
dc.description.abstractHuman immunoglobulin M (hIgM) antibodies are considered as hopeful tools for diseases therapy. Therefore, chromatography approaches are used to purify hIgM with a single step. In this study, we prepared a poly(hydroxyethyl methacrylate) based immunoaffinity p(HEMA-I) cryogel column by using cyanamide to immobilize antihuman immunoglobulin on the p(HEMA) cryogel for purification of hIgM in aqueous solution and artificial human plasma. The characterization of the p(HEMA) cryogel column was performed by using a scanning electron microscope (SEM), micro-computerized tomography (µ-CT), Fourier transform infrared spectroscopy (FTIR), swelling degree and macro-porosity. Further, the optimizations of various parameters were performed such as, pH, ionic strength, temperature and concentration of hIgM in aqueous solutions. In addition, the Langmuir adsorption model was supported by experimental results. Maximum adsorbed amount of hIgM corresponded to 11.1 mg/g at pH 5.75 [morpholino ethanesulfonic acid (MES buffer)]. Our results indicated that the p(HEMA-I) cryogel column can be reused at least 10 times without significant loss in adsorption capacity. As a natural source, artificial human plasma was selected for hIgM adsorption and the purity of hIgM was evaluated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).en_US
dc.identifier.citationBakhshpour, M., Topçu, A. A., Bereli, N. ve Alkan, H. (2020). Poly(Hydroxyethyl methacrylate) immunoaffinity cryogel column for the purification of human immunoglobulin M. Gels, 6(1), 4.en_US
dc.identifier.doi10.3390/gels6010004
dc.identifier.issn2310-2861
dc.identifier.issue1en_US
dc.identifier.pmid32013072
dc.identifier.scopus2-s2.0-85079891629
dc.identifier.scopusqualityQ2
dc.identifier.startpage4en_US
dc.identifier.urihttps://www.mdpi.com/2310-2861/6/1/4
dc.identifier.urihttps://hdl.handle.net/11468/7236
dc.identifier.volume6en_US
dc.identifier.wosWOS:000523677000006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAlkan, Huseyin
dc.language.isoenen_US
dc.publisherMDPI Multidisciplinary Digital Publishing Instituteen_US
dc.relation.ispartofGels
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAdsorptionen_US
dc.subjectCryogel columnen_US
dc.subjectHIgM purificationen_US
dc.subjectImmunoaffinityen_US
dc.titlePoly(Hydroxyethyl methacrylate) immunoaffinity cryogel column for the purification of human immunoglobulin Men_US
dc.titlePoly(Hydroxyethyl methacrylate) immunoaffinity cryogel column for the purification of human immunoglobulin M
dc.typeArticleen_US

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