Poly(Hydroxyethyl methacrylate) immunoaffinity cryogel column for the purification of human immunoglobulin M
Yükleniyor...
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
MDPI Multidisciplinary Digital Publishing Institute
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Human immunoglobulin M (hIgM) antibodies are considered as hopeful tools for diseases therapy. Therefore, chromatography approaches are used to purify hIgM with a single step. In this study, we prepared a poly(hydroxyethyl methacrylate) based immunoaffinity p(HEMA-I) cryogel column by using cyanamide to immobilize antihuman immunoglobulin on the p(HEMA) cryogel for purification of hIgM in aqueous solution and artificial human plasma. The characterization of the p(HEMA) cryogel column was performed by using a scanning electron microscope (SEM), micro-computerized tomography (µ-CT), Fourier transform infrared spectroscopy (FTIR), swelling degree and macro-porosity. Further, the optimizations of various parameters were performed such as, pH, ionic strength, temperature and concentration of hIgM in aqueous solutions. In addition, the Langmuir adsorption model was supported by experimental results. Maximum adsorbed amount of hIgM corresponded to 11.1 mg/g at pH 5.75 [morpholino ethanesulfonic acid (MES buffer)]. Our results indicated that the p(HEMA-I) cryogel column can be reused at least 10 times without significant loss in adsorption capacity. As a natural source, artificial human plasma was selected for hIgM adsorption and the purity of hIgM was evaluated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Açıklama
WOS:000523677000006
PMID: 32013072
PMID: 32013072
Anahtar Kelimeler
Adsorption, Cryogel column, HIgM purification, Immunoaffinity
Kaynak
Gels
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
6
Sayı
1
Künye
Bakhshpour, M., Topçu, A. A., Bereli, N. ve Alkan, H. (2020). Poly(Hydroxyethyl methacrylate) immunoaffinity cryogel column for the purification of human immunoglobulin M. Gels, 6(1), 4.
