Cu2+-attached pumice particles embedded composite cryogels for protein purification
[ X ]
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, chromatographic performance of Cu2+-attached pumice particles embedded to monolithic cryogels (Cu2+-APPsEMC) for human serum albumin (HSA) was investigated. Monolithic composite cryogels were prepared by means of polymerization of gel-forming precursors at sub-zero temperatures. The chemical composition of pumice and surface of composite cryogels were determined by X-ray fluorescence spectrometer and scanning electron microscopy, respectively. The highest adsorption capacity (549.5 mg/g pumice) of cryogels was achieved at phosphate buffer of pH 8.0 with initial HSA solution of 3 mg/ml. SDS-PAGE analysis was performed for the samples studied on human serum to determine HSA adsorption/desorption performance of cryogel qualitatively.
Açıklama
Anahtar Kelimeler
Composite Cryogel, Human Serum Albumin Chromatography, Metal Chelate, Particle Embedding, Pumice
Kaynak
Artificial Cells Nanomedicine and Biotechnology
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
45
Sayı
1
