Cu2+-attached pumice particles embedded composite cryogels for protein purification

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dc.contributor.authorAlkan, Hueseyin
dc.contributor.authorComert, Seyda Ceylan
dc.contributor.authorGurbuz, Fatma
dc.contributor.authorDogru, Mehmet
dc.contributor.authorOdabasi, Mehmet
dc.date.accessioned2024-04-24T17:20:36Z
dc.date.available2024-04-24T17:20:36Z
dc.date.issued2017
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn this study, chromatographic performance of Cu2+-attached pumice particles embedded to monolithic cryogels (Cu2+-APPsEMC) for human serum albumin (HSA) was investigated. Monolithic composite cryogels were prepared by means of polymerization of gel-forming precursors at sub-zero temperatures. The chemical composition of pumice and surface of composite cryogels were determined by X-ray fluorescence spectrometer and scanning electron microscopy, respectively. The highest adsorption capacity (549.5 mg/g pumice) of cryogels was achieved at phosphate buffer of pH 8.0 with initial HSA solution of 3 mg/ml. SDS-PAGE analysis was performed for the samples studied on human serum to determine HSA adsorption/desorption performance of cryogel qualitatively.en_US
dc.identifier.doi10.3109/21691401.2015.1129627
dc.identifier.endpage97en_US
dc.identifier.issn2169-1401
dc.identifier.issn2169-141X
dc.identifier.issue1en_US
dc.identifier.pmid26755205
dc.identifier.scopus2-s2.0-85003454027
dc.identifier.scopusqualityQ1
dc.identifier.startpage90en_US
dc.identifier.urihttps://doi.org/10.3109/21691401.2015.1129627
dc.identifier.urihttps://hdl.handle.net/11468/19156
dc.identifier.volume45en_US
dc.identifier.wosWOS:000391459800014
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofArtificial Cells Nanomedicine and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectComposite Cryogelen_US
dc.subjectHuman Serum Albumin Chromatographyen_US
dc.subjectMetal Chelateen_US
dc.subjectParticle Embeddingen_US
dc.subjectPumiceen_US
dc.titleCu2+-attached pumice particles embedded composite cryogels for protein purificationen_US
dc.titleCu2+-attached pumice particles embedded composite cryogels for protein purification
dc.typeArticleen_US

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