Effects of branched-chain amino acids on glucose uptake and lactose synthesis rates in bovine mammary epithelial cells and lactating mammary tissue slices

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dc.authorid0000-0001-8226-7603en_US
dc.contributor.authorSilva, J. V. V.
dc.contributor.authorGanesan, S.
dc.contributor.authorWickramasinghe, H. K. J. P.
dc.contributor.authorStepanchenko, N.
dc.contributor.authorKaya, C. A.
dc.contributor.authorBeitz, D. C.
dc.contributor.authorAppuhamy, J. A. D. R. N.
dc.date.accessioned2023-03-10T08:11:29Z
dc.date.available2023-03-10T08:11:29Z
dc.date.issued2022en_US
dc.departmentDicle Üniversitesi, Diyarbakır Tarım Meslek Yüksek Okulu, Bitkisel ve Hayvansal Üretim Bölümüen_US
dc.description.abstractEven though supplementations of essential AA (EAA) are often related to increased lactose yields in dairy cows, underlying mechanisms connecting EAA availability to the mammary glands and lactose synthesis are poorly understood. The objective of this study was to examine the effects of branched-chain AA (BCAA) including Leu, Ile, and Val on (1) glucose transporter (GLUT1) abundance and glucose uptake, (2) the abundance of proteins regulating lactose synthesis pathway, and (3) fractional synthesis rates of lactose (FSR) using bovine mammary epithelial cells (BMEC) and mammary tissues slices (MTS). The BMEC (n = 4) were allocated randomly to regular Dulbecco's Modified Eagle Medium with Ham's F12 (DMEM/F12) medium (+EAA) or +EAA deficient (by 90%) in all EAA (- EAA), all BCAA (-BCAA), only Leu (-Leu), only Ile (-Ile) or only Val (-Val). Western immunoblotting analyses, depletion of glucose in media, and a proteomic analysis were performed to determine the abundance of GLUT1 in the cell membrane, net glucose uptake, and the abundance of enzymes involved in lactose synthesis pathway in BMEC, respectively. The MTS (n = 6) were allocated randomly to DMEM/F12 medium having all EAA and C-13-glucose at concentrations similar to plasma concentrations of cows (+EAA(p)), and +EAA(p) deprived of all BCAA (-BCAA(p)) or only Leu (-Leup) for 3 h. The C-13 enrichments of free glucose pool in MTS (EGlu-free) and the enrichments of glucose incorporated into lactose in MTS and media [ELactose-bound ((T&M))] were determined and used in calculating FSR. In BMEC, -BCAA increased the fraction of total GLUT1 translocated to the cell membrane and the fraction that was potentially glycosylated compared with +EAA. Among individual BCAA, only-Leu was associated with a 63% increase in GLUT1 translocated to the cell membrane and a 40% increase in glucose uptake of BMEC. The-BCAA tended to be related to a 75% increase in the abundance of hexokinase in BMEC. Deprivation of Leu tended to increase glucose uptake of MTS but did not affect EGlu-free, ELactose-bound ((T&M)), or FSR relative to +EAA(p). On the other hand, -BCAA(p) did not affect glucose uptake of MTS but was related to lower ELactose-bound (T&M), or FSR relative to +EAA(p). Considering together, decreasing Leu supply to mammary tissues enhances GLUT1 and thus glucose uptake, which, however, does not affect lactose synthesis rates. Moreover, the deficiency of other BCAA, Ile, and Val alone or together with the deficiency of Leu seemed to decrease lactose synthesis rates without affecting glucose uptake. The data also emphasize the importance of addressing the effect of the supply of other nutrients to the mammary glands than the precursor supply in describing the synthesis of a milk component.en_US
dc.identifier.citationSilva, J. V. V., Ganesan, S., Wickramasinghe, H. K. J. P., Stepanchenko, N., Kaya, C. A., Beitz, D. C. ve diğerleri. (2022). Effects of branched-chain amino acids on glucose uptake and lactose synthesis rates in bovine mammary epithelial cells and lactating mammary tissue slices. Journal of Dairy Science, 105(2), 1717-1730.en_US
dc.identifier.doi10.3168/jds.2021-20950
dc.identifier.endpage1730en_US
dc.identifier.issn0022-0302
dc.identifier.issn1525-3198
dc.identifier.issue2en_US
dc.identifier.pmid34802743
dc.identifier.scopus2-s2.0-85119408217
dc.identifier.scopusqualityQ1
dc.identifier.startpage1717en_US
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0022030221010389?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/11468/11358
dc.identifier.volume105en_US
dc.identifier.wosWOS:000746143000003
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKaya, Can Ayhan
dc.language.isoenen_US
dc.publisherElsevier Science INC.en_US
dc.relation.ispartofJournal of Dairy Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectIsoleucineen_US
dc.subjectLeucineen_US
dc.subjectHexokinaseen_US
dc.subjectN-glycosylationen_US
dc.titleEffects of branched-chain amino acids on glucose uptake and lactose synthesis rates in bovine mammary epithelial cells and lactating mammary tissue slicesen_US
dc.titleEffects of branched-chain amino acids on glucose uptake and lactose synthesis rates in bovine mammary epithelial cells and lactating mammary tissue slices
dc.typeArticleen_US

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