Targeting soluble guanylate cyclase with Riociguat has potency to alleviate testicular ischaemia reperfusion injury via regulating various cellular pathways

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dc.authorid0000-0002-1693-6378en_US
dc.authorid0000-0002-9681-4468en_US
dc.authorid0000-0002-4403-8663en_US
dc.authorid0000-0002-2504-120Xen_US
dc.authorid0000-0002-4760-3306en_US
dc.authorid0000-0002-3059-9398en_US
dc.authorid0000-0001-6160-5626en_US
dc.authorid0000-0001-9496-7324en_US
dc.contributor.authorŞeker, Uğur
dc.contributor.authorKavak, Deniz Evrim
dc.contributor.authorGüzel, Barış Can
dc.contributor.authorBaygeldi, Saime Betül
dc.contributor.authorYüksel, Meral
dc.contributor.authorKandemir, Sevgi İrtegün
dc.contributor.authorŞener, Dila
dc.contributor.authorDemirel, Özlem Unay
dc.date.accessioned2024-03-19T13:22:34Z
dc.date.available2024-03-19T13:22:34Z
dc.date.issued2022en_US
dc.departmentDicle Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Ana Bilim Dalıen_US
dc.description.abstractTesticular ischaemia reperfusion (I/R) injury results with serious dysfunctions in testis. This study aims to explore effects of soluble guanylate cyclase (sGC) stimulator Riociguat on experimental testicular I/R injury in rats. Twenty-one male rats were divided into three groups (Control, IR and IRR). The control group was not exposed to any application. Bilateral testis from IR and IRR animals were rotated 720° in opposite directions for 3 h to induce experimental testicular ischaemia. Animals in IR and IRR groups were subjected to 3 h of reperfusion. Isotonic and Riociguat were administered to the animals 30 min prior reperfusion by oral gavage. At the end of experiment, animals were sacrificed and tissue samples were used for analyses. Riociguat treatment significantly decreased tissue malondialdehyde and Luminol levels compared to the IR group (p < 0.05). The pathological changes, pro-apoptotic proteins (Bax, Caspase 3, and Caspase 9) and apoptotic index in the IR group were down regulated in Riociguat treated animals (p < 0.05). Riociguat treatment was also significantly increased anti-apoptotic Bcl-2 expression, but alleviated tissue injury via modulating pro-inflammatory cytokine IL-1β levels and significantly (p < 0.05) down-regulating NF-κB activity. Moreover, mTOR and ERK phosphorylation increased in IR group (p < 0.05), but Riociguat treatment reduced protein phosphorylation. Our experiment indicated that targeting sGC might support surgical interventions in testicular I/R injury by modulating oxidative stress, inflammation, and apoptotic protein expression levels, but more detailed studies are required to explore the protective activity of Riociguat and underlying mechanisms in testicular I/R injury.en_US
dc.identifier.citationŞeker, U., Kavak, D. E., Güzel, B. C., Baygeldi, S. B., Yüksel, M., Demirel, Ö. U. ve diğerleri. (2022). Targeting soluble guanylate cyclase with Riociguat has potency to alleviate testicular ischaemia reperfusion injury via regulating various cellular pathways. Andrologia, 54(11), 1-11.en_US
dc.identifier.doi10.1111/and.14616
dc.identifier.endpage11en_US
dc.identifier.issn0303-4569
dc.identifier.issue11en_US
dc.identifier.pmid36283690
dc.identifier.scopus2-s2.0-85140399446
dc.identifier.scopusqualityQ1
dc.identifier.startpage1en_US
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/epdf/10.1111/and.14616
dc.identifier.urihttps://hdl.handle.net/11468/13648
dc.identifier.volume54en_US
dc.identifier.wosWOS:000875051400001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKandemir, Sevgi İrtegün
dc.language.isoenen_US
dc.publisherJohn Wiley and Sons Incen_US
dc.relation.ispartofAndrologia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIschaemia reperfusion injuryen_US
dc.subjectRaten_US
dc.subjectRiociguaten_US
dc.subjectsGC stimulatoren_US
dc.subjectTestisen_US
dc.titleTargeting soluble guanylate cyclase with Riociguat has potency to alleviate testicular ischaemia reperfusion injury via regulating various cellular pathwaysen_US
dc.titleTargeting soluble guanylate cyclase with Riociguat has potency to alleviate testicular ischaemia reperfusion injury via regulating various cellular pathways
dc.typeArticleen_US

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