Optimum processing conditions for a trivalent-inactivated bovine viral diarrhea virus (BVDV) vaccine using field strains and immunogenicity of candidate formulations with different adjuvants

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dc.authorid0000-0001-5969-6127en_US
dc.authorid0000-0003-1793-6879en_US
dc.contributor.authorKadiroğlu, Berfin
dc.contributor.authorYeşilbağ, Kadir
dc.date.accessioned2025-02-19T10:42:08Z
dc.date.available2025-02-19T10:42:08Z
dc.date.issued2025en_US
dc.departmentDicle Üniversitesi, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümü, Viroloji Ana Bilim Dalıen_US
dc.description.abstractBovine viral diarrhea virus (BVDV) is among the common bovine pathogens worldwide. One of the prominent protection measures of BVDV is vaccination. This study aimed to determine the growth characteristics, inactivation kinetics of vaccine candidates using local BVDV strains [TR-26 (BVDV-1f), TR-21 (BVDV-1l), and TR-15 (BVDV-2b)], and the serological response in experimental animals to inactivated BVDV vaccine formulations prepared with different adjuvants. Optimum MOI values for BVDV strains TR-26, TR-21, and TR-15 were determined as 0.1, 1.0, and 0.01, respectively. In addition, growth curves of TR-26, TR-21, and TR-15 strains were created, and it was determined that they reached the highest titers at 12, 48, and 36 h p.i., respectively. The strains TR-26, TR-21, and TR-15 with titers of 106.5, 106.5, and 105.25 TCID50/ml were completely inactivated by 1 mM binary ethyleneimine (BEI) at the 10th, 16th, and 10th hours of treatment, respectively. Guinea pigs were immunized with four vaccine formulations (F1, F2, F3, F4), two with aluminum-based [Al(OH)3, Al(OH)3+Saponin] and two with oil-based (ISA 50 and ISA 206) adjuvants. Neutralization tests were applied to determine the humoral immune response developed after vaccination. Both homologous and heterologous BVDV strains were used for evaluations. Oil adjuvanted vaccines were more efficient to induce antibody titers compared to Al(OH)3-based vaccines. In addition, between the oil adjuvanted vaccines, the titers of neutralizing antibodies obtained by Montanide® ISA 206 formulation were significantly higher than in Montanide® ISA 50 (p < 0.05). Post-vaccinal neutralizing antibodies were detected in the first sampling at 21st day and lasted longer than a 111 days period. The highest antibody response in Guinea pigs was for the strain TR-15. The availability of using BVDV-lf, 1l, and 2b local strains in vaccines and their effectiveness against homologous and heterologous strains have been demonstrated.en_US
dc.description.sponsorshipTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) 119 O 571en_US
dc.identifier.citationKadiroğlu, B. ve Yeşilbağ, K. (2025). Optimum processing conditions for a trivalent-inactivated bovine viral diarrhea virus (BVDV) vaccine using field strains and immunogenicity of candidate formulations with different adjuvants. Veterinary Research Communications, 49(1), 1-9.en_US
dc.identifier.endpage9en_US
dc.identifier.issn0165-7380
dc.identifier.issue1en_US
dc.identifier.pmid9589623
dc.identifier.scopus2-s2.0-85210252596
dc.identifier.scopusqualityQ2
dc.identifier.startpage1en_US
dc.identifier.urihttps://link.springer.com/article/10.1007/s11259-024-10608-5
dc.identifier.urihttps://hdl.handle.net/11468/29469
dc.identifier.volume49en_US
dc.identifier.wosWOS:001364898300004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.indekslendigikaynakScopus
dc.institutionauthorKadiroğlu, Berfin
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media B.V.en_US
dc.relation.ispartofVeterinary Research Communications
dc.relation.isversionof10.1007/s11259-024-10608-5en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBVDVen_US
dc.subjectGrowth curveen_US
dc.subjectGuinea pigen_US
dc.subjectImmunizationen_US
dc.subjectInactivated vaccineen_US
dc.subjectInactivation kineticsen_US
dc.titleOptimum processing conditions for a trivalent-inactivated bovine viral diarrhea virus (BVDV) vaccine using field strains and immunogenicity of candidate formulations with different adjuvantsen_US
dc.titleOptimum processing conditions for a trivalent-inactivated bovine viral diarrhea virus (BVDV) vaccine using field strains and immunogenicity of candidate formulations with different adjuvants
dc.typeArticleen_US

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