The toxicity of dithiocarbamate fungicides to soil nematodes, assessed using a stress-inducible transgenic strain of Caenorhabditis elegans
Yükleniyor...
Tarih
1999
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
John Wiley and Sons Inc.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The dithiocarbamate fungicides maneb and mancozeb induce a short-term stress response in a transgenic Caenorhabditis elegans strain (PC72) carrying a reporter lacZ gene under the control of a homologous heat shock (hsp16) promoter. This response can be readily monitored as induced beta-galactosidase activity, either by in situ staining or by a quantitative fluorometric enzyme assay. Particularly strong responses are induced by mancozeb (three- to fivefold above controls at 500 pg mL(-1)), causing acute toxicity at concentrations comparable to those recommended for field application (2 mg mL(-1)). Although much of this fungicide is adsorbed by soil, sufficient (ca. 6%) enters the soil water compartment to cause mild stress in the transgenic worm assay. Among possible metabolites from mancozeb breakdown, neither Mn2+ nor ethylenethiourea (ETU) is particularly toxic even at 10% of the optimum mancozeb dosage. Stress responses to a range of other pesticides are also reported, and in several cases it is clear that a nontarget soil species there, transgenic C. elegans) may be sensitive to low-level contamination. (C) 1999 John Wiley & Sons, Inc.
Açıklama
Anahtar Kelimeler
Nematode, Toxicity, Transgenic, Fungicide, Dithiocarbamate, Stress inducible, Heat shock response
Kaynak
Journal of Biochemical and Molecular Toxicology
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
13
Sayı
6
Künye
Güven, K., Power, R. S., Avramides, S., Allender, R. ve De Pomerai, D. I. (1999). The toxicity of dithiocarbamate fungicides to soil nematodes, assessed using a stress-inducible transgenic strain of Caenorhabditis elegans. Journal of Biochemical and Molecular Toxicology, 13(6), 324-333.
