Simultaneous determination of ascorbic acid and caffeine in commercial soft drinks using reversed-phase ultraperformance liquid chromatography

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dc.contributor.authorTurak, Fatma
dc.contributor.authorGuzel, Remziye
dc.contributor.authorDinc, Erdal
dc.date.accessioned2024-04-24T16:15:05Z
dc.date.available2024-04-24T16:15:05Z
dc.date.issued2017
dc.departmentDicle Üniversitesien_US
dc.description.abstractA new reversed-phase ultraperformance liquid chromatography method with a photodiode array detector was developed for the quantification of ascorbic acid (AA) and caffeine (CAF) in 11 different commercial drinks consisting of one energy drink and 10 ice tea drinks. Separation of the analyzed AA and CAF with an internal standard, caffeic acid, was performed on a Waters BEH C-18 column (100 mm x 2.1 mm, 1.7 mu m i.d.), using a mobile phase consisting of acetonitrile and 0.2M H3PO4 (11: 89, v/v) with a flow rate of 0.25 mL/min and an injection volume of 1.0 mu L. Calibration graphs for AA and CAF were computed from the peak area ratio of AA/internal standard and CAF/internal standard detected at 244.0 nm and 273.6 nm, respectively. The developed reversed-phase ultraperformance liquid chromatography method was validated by analyzing standard addition samples. The proposed reversed-phase ultraperformance liquid chromatography method gave us successful results for the quantitative analysis of commercial drinks containing AA and CAF substances. Copyright (C) 2016, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.en_US
dc.identifier.doi10.1016/j.jfda.2016.09.004
dc.identifier.endpage292en_US
dc.identifier.issn1021-9498
dc.identifier.issue2en_US
dc.identifier.pmid28911669
dc.identifier.scopus2-s2.0-85006994766
dc.identifier.scopusqualityQ1
dc.identifier.startpage285en_US
dc.identifier.urihttps://doi.org/10.1016/j.jfda.2016.09.004
dc.identifier.urihttps://hdl.handle.net/11468/15639
dc.identifier.volume25en_US
dc.identifier.wosWOS:000399338100008
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherFood & Drug Adminstrationen_US
dc.relation.ispartofJournal of Food and Drug Analysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAscorbic Aciden_US
dc.subjectCaffeineen_US
dc.subjectCommercial Soft Drinksen_US
dc.subjectQuantificationen_US
dc.subjectReverse-Phase Ultraperformanceen_US
dc.subjectLiquid Chromatographyen_US
dc.titleSimultaneous determination of ascorbic acid and caffeine in commercial soft drinks using reversed-phase ultraperformance liquid chromatographyen_US
dc.titleSimultaneous determination of ascorbic acid and caffeine in commercial soft drinks using reversed-phase ultraperformance liquid chromatography
dc.typeArticleen_US

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