Simultaneous determination of ascorbic acid and caffeine in commercial soft drinks using reversed-phase ultraperformance liquid chromatography
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Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Food & Drug Adminstration
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
A new reversed-phase ultraperformance liquid chromatography method with a photodiode array detector was developed for the quantification of ascorbic acid (AA) and caffeine (CAF) in 11 different commercial drinks consisting of one energy drink and 10 ice tea drinks. Separation of the analyzed AA and CAF with an internal standard, caffeic acid, was performed on a Waters BEH C-18 column (100 mm x 2.1 mm, 1.7 mu m i.d.), using a mobile phase consisting of acetonitrile and 0.2M H3PO4 (11: 89, v/v) with a flow rate of 0.25 mL/min and an injection volume of 1.0 mu L. Calibration graphs for AA and CAF were computed from the peak area ratio of AA/internal standard and CAF/internal standard detected at 244.0 nm and 273.6 nm, respectively. The developed reversed-phase ultraperformance liquid chromatography method was validated by analyzing standard addition samples. The proposed reversed-phase ultraperformance liquid chromatography method gave us successful results for the quantitative analysis of commercial drinks containing AA and CAF substances. Copyright (C) 2016, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.
Açıklama
Anahtar Kelimeler
Ascorbic Acid, Caffeine, Commercial Soft Drinks, Quantification, Reverse-Phase Ultraperformance, Liquid Chromatography
Kaynak
Journal of Food and Drug Analysis
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
25
Sayı
2
