Culture and culture-independent diagnostic tests in Campylobacter enteritis

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dc.authorid0000-0001-6898-7516en_US
dc.authorid0000-0002-2579-1932en_US
dc.contributor.authorÖzcan, Nida
dc.contributor.authorBacalan, Fatma
dc.contributor.authorÇakır, Fatih
dc.contributor.authorBilden, Alican
dc.contributor.authorGenişel, Neslihan
dc.contributor.authorDal, Tuba
dc.date.accessioned2023-07-06T06:22:47Z
dc.date.available2023-07-06T06:22:47Z
dc.date.issued2022en_US
dc.departmentDicle Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalıen_US
dc.description.abstractIntroduction: Campylobacter infections are among the most common causes of bacterial enteritis. This study aims to determine the sensitivity, specificity and positive predictive values (PPV) of culture and culture-independent tests for the diagnosis of Campylobacter enteritis. Methodology: A total of 400 stool samples were included in the study. BD MAX enteric bacterial panel (BD Diagnostics, Franklin Lakes, NJ, USA) and EntericBio Gastro Panel II (Serosep, Limerick, Ireland) were used as commercial molecular tests. RIDA??QUICK Campylobacter (R-Biopharm, Darmstadt,, Germany) and CerTest (Biotec, Zaragoza, Spain) were used to detect Campylobacter antigens. Samples were cultured in CCDA media and subjected to bacterial identification by mass spectrometry. Results: Among the 400 specimens, 41 (10.2%) were evaluated as Campylobacter positive; 21 were culture-positive and 20 were detected as positive by both PCR methods. Of the 21 isolates grown in culture, 16 (76.2%) were identified as C. jejuni and 5 (23.8%) as C. coli. While all 21 culture-positive specimens were detected as positive by both molecular tests, 18 of the specimens were found positive by RidaQuick, and 16 by Certest ICA. Of the 20 culture-negative Campylobacter cases, 18 were positive by RidaQuick and 12 by Certest ICA. Sensitivities of culture, ICA-RidaQuick and ICA-CerTest were 51.2%, 87.8 and 68.3, respectively. The specificities of all tests were in the range of 90-100 %. PPV of molecular tests, ICA-RidaQuick and ICA-CerTest were > 95%, 72 % and 48.3 %, respectively. Conclusions: Molecular tests were superior to culture and ICA in terms of sensitivity, specificity, and positive predictive value.en_US
dc.identifier.citationÖzcan, N., Bacalan, F., Çakır, F., Bilden, A., Genişel, N. ve Dal, T. (2022). Culture and culture-independent diagnostic tests in Campylobacter enteritis. Journal of Infection Developing Countries, 16(4), 616-621.en_US
dc.identifier.doi10.3855/jidc.14902
dc.identifier.endpage621en_US
dc.identifier.issn1972-2680
dc.identifier.issue4en_US
dc.identifier.pmid35544622
dc.identifier.scopus2-s2.0-85129909250
dc.identifier.scopusqualityQ2
dc.identifier.startpage616en_US
dc.identifier.urihttps://www.jidc.org/index.php/journal/article/view/35544622/2796
dc.identifier.urihttps://hdl.handle.net/11468/12197
dc.identifier.volume16en_US
dc.identifier.wosWOS:000798597600007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzcan, Nida
dc.institutionauthorGenişel, Neslihan
dc.language.isoenen_US
dc.publisherJournal of Infection Developing Countriesen_US
dc.relation.ispartofJournal of Infection Developing Countries
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCampylobacteren_US
dc.subjectGastroenteritisen_US
dc.subjectMultiplex PCRen_US
dc.subjectImmunochromatographyen_US
dc.titleCulture and culture-independent diagnostic tests in Campylobacter enteritisen_US
dc.titleCulture and culture-independent diagnostic tests in Campylobacter enteritis
dc.typeArticleen_US

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