Effects of formaldehyde on vascular endothelial growth factor, matrix metallopeptidase 2 and osteonectin levels in periodontal membrane and alveolar bone in rats

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dc.authorid0000-0001-7317-5467en_US
dc.authorid0000-0002-1967-4844en_US
dc.authorid0000-0002-2353-1184en_US
dc.contributor.authorLaçin, Nihat
dc.contributor.authorİzol, Bozan Serhat
dc.contributor.authorTuncer, Cudi Mehmet
dc.contributor.authorÖzkorkmaz, Ebru Gokalp
dc.contributor.authorDeveci, Buşra
dc.contributor.authorDeveci, Engin
dc.date.accessioned2021-11-05T10:55:43Z
dc.date.available2021-11-05T10:55:43Z
dc.date.issued2019en_US
dc.departmentDicle Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Anatomi Ana Bilim Dalıen_US
dc.description.abstractackground: The objective of this study was to investigate whether long term formaldehyde inhalation may affect periodontal membrane and alveolar bone loss leading to periodontitis. The negative effects of formaldehyde were described using vascular endothelial growth factor (VEGF), matrix metallopeptidase 2 (MMP-2) and osteonectin antibodies involved in the extracellular matrix and angiogenetic development. Materials and methods: Thirty adult Wistar albino rats were used in this study. Rats were divided into two groups: a control group (n = 15) and formaldehyde administered group (n = 15). Formaldehyde group was exposed to inhalation of 10 ppm formaldehyde 8 hours a day, 5 days a week for 5 weeks. Maxillary bone regions were dissected under anaesthesia. After fixation in 10% formaldehyde solution, tissues were passed through graded ethanol series to obtain paraffin blocks. Five-micrometre histological sections were cut with RM2265 rotary microtome stained with Masson trichrome and VEGF, MMP-2 and osteonectin antibodies for examination under Olympus BH-2 light microscopy. Results: The present study revealed that congestion in blood vessels, degeneration of collagen fibres and alveolar matrix around alveolar bone were observed to be more significant in formaldehyde group than the control group (p ≤ 0.001). Interestingly, VEGF expression in the formaldehyde group was the most significant finding between the two groups (p < 0.001). When compared inflammation, MMP-2 and osteonectin expressions were significant (p < 0.01) in the formaldehyde group. Conclusions: It was suggested that formaldehyde toxicity decreased the expression of MMP-2 and in osteoblasts as well as affecting the retention of MMP levels in tooth cavity, which is very low in collagen fibres. But, vice versa for the expression of VEGF in dilated vascular endothelial cells and osteocytes in alveolar bone. As a conclusion, formaldehyde disrupts the periodontal membrane and may cause collagen fibres degeneration by affecting the alveolar bone matrix.en_US
dc.identifier.citationLaçin, N., İzol, B. S., Tuncer, C. M., Özkorkmaz, E. G., Deveci, B. ve Deveci, E. (2019). Effects of formaldehyde on vascular endothelial growth factor, matrix metallopeptidase 2 and osteonectin levels in periodontal membrane and alveolar bone in rats. Folia Morphologica (Poland), 78(3), 545-553.en_US
dc.identifier.doi10.5603/FM.a2018.0110
dc.identifier.endpage553en_US
dc.identifier.issn0015-5659
dc.identifier.issue3en_US
dc.identifier.pmid30536359
dc.identifier.scopus2-s2.0-85063739550
dc.identifier.scopusqualityQ2
dc.identifier.startpage545en_US
dc.identifier.urihttps://journals.viamedica.pl/folia_morphologica/article/view/60381
dc.identifier.urihttps://hdl.handle.net/11468/8132
dc.identifier.volume78en_US
dc.identifier.wosWOS:000484118100012
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTuncer, Cudi Mehmet
dc.institutionauthorÖzkorkmaz, Ebru Gökalp
dc.institutionauthorDeveci, Buşra
dc.institutionauthorDeveci, Engin
dc.language.isoenen_US
dc.publisherVia Medicaen_US
dc.relation.ispartofFolia Morphologica (Poland)
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAlveolar boneen_US
dc.subjectFormaldehydeen_US
dc.subjectImmunohistochemistryen_US
dc.subjectPeriodontal membraneen_US
dc.titleEffects of formaldehyde on vascular endothelial growth factor, matrix metallopeptidase 2 and osteonectin levels in periodontal membrane and alveolar bone in ratsen_US
dc.titleEffects of formaldehyde on vascular endothelial growth factor, matrix metallopeptidase 2 and osteonectin levels in periodontal membrane and alveolar bone in rats
dc.typeArticleen_US

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