Metabolomics Approach to Explore Bioactive Natural Products Derived From Plant-Root-Associated Streptomyces

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dc.contributor.authorKum, Ekrem
dc.contributor.authorInce, Ebru
dc.date.accessioned2024-04-24T16:02:36Z
dc.date.available2024-04-24T16:02:36Z
dc.date.issued2024
dc.departmentDicle Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Ana Bilim Dalıen_US
dc.description.abstractStreptomyces, a prominent genus within the Actinomycetota phylum, is responsible for over 60% of clinically relevant antibiotics. Streptomyces strains inhabiting plant roots possess the potential to synthesize bioactive natural products, conferring defense and resilience to plants against pathogenic microorganisms. However, this potential remains largely unexplored. This study aims to screen for bioactive metabolites produced by Streptomyces strains in the plant rhizosphere. Six Streptomyces isolates were cultivated using three modified media to induce the production of diverse metabolites, employing the One Strain Many Compounds (OSMAC) approach. The metabolites present in extracts from fermentation broths were examined through a non-targeted Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) approach coupled with Global Natural Products Social Molecular Networking (GNPS MN). The antimicrobial activity of the extracts was assessed using the disc diffusion method. The strains demonstrated a wide-ranging antimicrobial efficacy against all examined organisms. The GNPS molecular network analyses reveal that metabolite profiles in extracts can exhibit variations based on the medium and solvent system employed. Notably, the ethyl acetate and dichloromethane extracts from Streptomyces sp. CAH29, cultivated in Glucose-Yeast Extract Medium (GYM), exhibited inhibition diameters of up to 30 mm against both Staphylococcus aureus and Candida albicans. Within the metabolomes of these strains, the antibiotics spiramycin and actinomycin were detected. Additionally, lyngbatoxin, a tumor promoter, and potential new analogs were identified. Significantly, a considerable portion of the produced metabolites did not align with any known compounds, indicating the existence of unidentified metabolites generated by these strains. This suggests the possibility of introducing novel chemical entities. Our study illustrated that Streptomyces strains associated with plant roots could be considered a valuable source of bioactive secondary metabolites. Furthermore, the metabolomics approach utilized in this study serves as a rapid and valuable tool for the screening of microorganisms capable of producing bioactive metabolites.en_US
dc.description.sponsorshipDicle niversitesien_US
dc.description.sponsorshipNo Statement Availableen_US
dc.identifier.citationKum, E. ve İnce, E. (2024). Metabolomics approach to explore bioactive natural products derived from plant-root-associated streptomyces. Applied Biochemistry and Biotechnology, 1-14.
dc.identifier.doi10.1007/s12010-024-04905-7
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.pmid38512549en_US
dc.identifier.scopus2-s2.0-85188246232en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1007/s12010-024-04905-7
dc.identifier.urihttps://hdl.handle.net/11468/14855
dc.identifier.wosWOS:001188124200001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofApplied Biochemistry and Biotechnologyen_US
dc.relation.journalApplied Biochemistry and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNatural productsen_US
dc.subjectRoot-associated streptomycesen_US
dc.subjectAntimicrobialsen_US
dc.subjectMetabolomicsen_US
dc.titleMetabolomics Approach to Explore Bioactive Natural Products Derived From Plant-Root-Associated Streptomycesen_US
dc.typeArticleen_US

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