Altered luteal expression patterns of genomic and non-genomic progesterone receptors in bitches at different reproductive states

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dc.authorid0000-0002-8988-3158en_US
dc.authorid0000-0001-5636-061Xen_US
dc.authorid0000-0002-2150-0640en_US
dc.authorid0000-0003-0070-8458en_US
dc.authorid0000-0002-5707-8832en_US
dc.authorid0000-0001-9853-5334en_US
dc.contributor.authorUçar, Eyyüp Hakan
dc.contributor.authorPeker, Cevdet
dc.contributor.authorHitit, Mustafa
dc.contributor.authorKöse, Mehmet
dc.contributor.authorTatar, Musa
dc.contributor.authorBozkaya, Faruk
dc.contributor.authorAtlı, Mehmet Osman
dc.date.accessioned2024-04-05T13:00:56Z
dc.date.available2024-04-05T13:00:56Z
dc.date.issued2024en_US
dc.departmentDicle Üniversitesi, Veteriner Fakültesi, Klinik Bilimler Bölümü, Doğum ve Jinekoloji Ana Bilim Dalıen_US
dc.description.abstractThe binding of steroid hormones to their specific receptors is necessary to exert their effects on target cells. Progesterone (P4), a steroid hormone, carries out its effects through both genomic and non-genomic (the cell membrane-associated) receptors. This study aimed to ascertain luteal expression patterns of genomic and non-genomic progesterone receptors in bitches in physiological (early dioestrus and early pregnant) and pathological (pyometra) reproductive states. Luteal tissue was collected from the bitches at early dioestrus (ED, n = 5), early pregnant (EP, n = 5), and pyometra (PY, n = 5). The expression profiles of Steroidogenic Acute Regulator Protein (STAR), Progesterone Receptor (PGR), Membrane Progestin Receptors (PAQR5, PAQR7 and PAQR8), and Progesterone Membrane Components (PGMRC1 and PGMRC2) were examined at the mRNA levels using Real-Time Polymerase Chain Reaction (RT-PCR). Protein levels of PGR, PGMRC1 and PGMRC2 were detected by western blotting (WB). The STAR expression was found in all groups, with a statistical difference observed between EP and PY groups (P < 0.05). The protein level of PGR was determined to be highest in the EP group and lowest in the PY group. The expression of PAQR8 increased in the EP group (P < 0.05). The PAQR5 exhibited high expression in the EP group and low expression in the PY group (P < 0.05). PGRMC1 was more elevated in the EP group and lower in the PY group (P < 0.05). Protein levels of PGMRC1 and PGMRC2 were also observed at the highest expression in EP group. According to the altered expression profiles for examined receptors, we suggest that those progesterone receptors have roles in early pregnancy or pyometra in bitches.en_US
dc.identifier.citationUçar, E. H., Peker, C., Hitit, M., Köse, M., Tatar, M., Bozkaya, F. ve diğerleri. (2024). Altered luteal expression patterns of genomic and non-genomic progesterone receptors in bitches at different reproductive states. Theriogenology, 218, 153-162.en_US
dc.identifier.doi10.1016/j.theriogenology.2024.02.001
dc.identifier.endpage162en_US
dc.identifier.issn0093-691X
dc.identifier.pmid38325152
dc.identifier.scopus2-s2.0-85183950805
dc.identifier.scopusqualityQ1
dc.identifier.startpage153en_US
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0093691X24000591?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/11468/13861
dc.identifier.volume218en_US
dc.identifier.wosWOS:001182486500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKöse, Mehmet
dc.language.isoenen_US
dc.publisherElsevier Inc.en_US
dc.relation.ispartofTheriogenology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBitchesen_US
dc.subjectEarly pregnancyen_US
dc.subjectGenomic and non-genomic progesterone receptorsen_US
dc.subjectPyometraen_US
dc.titleAltered luteal expression patterns of genomic and non-genomic progesterone receptors in bitches at different reproductive statesen_US
dc.titleAltered luteal expression patterns of genomic and non-genomic progesterone receptors in bitches at different reproductive states
dc.typeArticleen_US

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