Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS

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dc.authorid0000-0003-4163-9962en_US
dc.contributor.authorBektaş, Nurdan Yazıcı
dc.contributor.authorErsoy, Ezgi
dc.contributor.authorBoğa, Mehmet
dc.contributor.authorBoran, Tuğçe
dc.contributor.authorÇınar, Ercan
dc.contributor.authorÖzhan, Gül
dc.contributor.authorGören, Ahmet Ceyhan
dc.contributor.authorÖzkan, Esra Eroğlu
dc.date.accessioned2021-11-11T13:32:41Z
dc.date.available2021-11-11T13:32:41Z
dc.date.issued2021en_US
dc.departmentDicle Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümüen_US
dc.descriptionWOS:000709117200027
dc.description.abstractThe study aims to determine the secondary metabolites of Hypericum androsaemum L. extracts by liquid chromatography-high resolution mass spectrometry (LC-HRMS), and investigate the antioxidant and cytotoxic activities of the plant. Cytotoxic activity was evaluated by MTT assay, and apoptosis induction abilities on human prostate adenocarcinoma (PC-3), and hepatocellular carcinoma (Hep G2) cell lines. Accordingly, major secondary metabolites were found as hederagenin (762 +/- 70.10 mu g/g) in the leaves dichloromethane (LD), herniarin (167 +/- 1.50 mu g/g) in fruit dichloromethane (FD), (-)-epicatechin (6538 +/- 235.36 mu g/g) in the leaves methanol (LM), (-)-epigallocatechin gallate (758 +/- 20.46 mu g/g) in the fruit methanol (FM), and caffeic acid (370 +/- 8.88 mu g/g) in the fruit water (FW), and (3313 +/- 79.51 mu g/g) in the leaves water (LW) extracts. LM exerted strong antioxidant activity in DPPH free (IC50 10.94 +/- 0.08 mu g/mL), and ABTS cation radicals scavenging (IC50 9.09 +/- 0.05 mu g/mL) activities. FM exhibited cytotoxic activity with IC50 values of 73.23 +/- 3.06 mu g/mL and 31.64 +/- 2.75 mu g/mL on PC-3 and Hep G2 cell lines, respectively. Being the richest extract in terms of quillaic acid (630 +/- 18.9 mu g/g), which is a well-known cytotoxic triterpenoid with proven apoptosis induction ability on different cells, FM extract showed apoptosis induction activity with 64.75% on PC-3 cells at 50 mu g/mL concentration. The study provides promising results about the potential of Hypericum androsaemum on cancer prevention.en_US
dc.identifier.citationBektaş, N.Y., Ersoy, E., Boğa, M., Boran, T., Çınar, E., Özhan, G. ve diğerleri. (2021). Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS. Turkish Journal of Chemistry, 45(5), 1621-1638.en_US
dc.identifier.doi10.3906/kim-2104-17
dc.identifier.endpage1638en_US
dc.identifier.issn1300-0527
dc.identifier.issue5en_US
dc.identifier.pmid34849072
dc.identifier.scopus2-s2.0-85117598781
dc.identifier.scopusqualityQ3
dc.identifier.startpage1621en_US
dc.identifier.trdizinid527695
dc.identifier.urihttps://journals.tubitak.gov.tr/chem/issues/kim-21-45-5/kim-45-5-27-2104-17.pdf
dc.identifier.urihttps://hdl.handle.net/11468/8237
dc.identifier.volume45en_US
dc.identifier.wosWOS:000709117200027
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorBoğa, Mehmet
dc.language.isoenen_US
dc.publisherScientific Technical Research Council Turkey-TUBITAKen_US
dc.relation.ispartofTurkish Journal of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHypericum androsaemumen_US
dc.subjectCytotoxicen_US
dc.subjectApoptosisen_US
dc.subjectQuillaic aciden_US
dc.subjectAntioxidanten_US
dc.subjectLC-HRMSen_US
dc.titleCytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMSen_US
dc.titleCytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS
dc.typeArticleen_US

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