Neuroprotective effects of allopurinol on spinal cord injury in rats: A biochemical and immunohistochemical study

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dc.authorid0000-0002-1967-4844en_US
dc.contributor.authorBaloǧlu, Murat
dc.contributor.authorÖzkorkmaz, Ebru Gökalp
dc.date.accessioned2022-03-02T12:16:14Z
dc.date.available2022-03-02T12:16:14Z
dc.date.issued2019en_US
dc.departmentDicle Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalıen_US
dc.description.abstractBackground: Lesion in spinal cord causes a cascade of events such as the apoptosis of neurons and eventually, neurological dysfunction. Neurologic damage developing after acute spinal cord injury is also related with necrosis and free radical formation. Allopurinol, a xanthine oxidase inhibitor, was shown to have protective effects in several studies. B-cell lymphoma 2 (Bcl-2) family proteins regulate apoptosis. Apoptosis causes the death of neuronal cells, particularly neurons and oligodendrocytes in the spinal cord after lesion. Glial fibrillary acidic protein (GFAP) takes part in astrocyte and neuronal interconnection and synaptic transmission. Materials and methods: Male Sprague Dawley rats (n = 30) were divided as control, trauma, and trauma + allopurinol (i.p., 50 mg/kg of body weight) groups. Animals were applied a surgical procedure causing spinal cord injury and treated for 7 days then sacrificed under anaesthesia. The spinal cords were dissected, measurements of myeloperoxidase, malondialdehyde and glutathione were performed, remaining parts were fixed in 10% formaldehyde solution for histological and immunohistochemical evaluations. Results: Biochemical results exhibited an increase in myeloperoxidase levels in trauma group but a decrease in the allopurinol treatment group similar to malondialdehyde levels. Degenerative changes in multipolar and bipolar neurons together with apoptotic changes in some glial cells were observed in the trauma group whereas, mild degenerative changes were observed after allopurinol treatment. In the trauma group, negative GFAP expression in multipolar versus bipolar neuronal processes with a reduction in glial processes around blood vessels and positive GFAP expression were observed but, a regular and parallel positive GFAP expression of glial processes around blood vessels in the allopurinol treated group was apparent. Trauma group depicted a positive Bcl-2 expression in glial cells and in motor and bipolar neurons. On the contrary, negative Bcl-2 expression was noticed in the trauma + allopurinol group. Conclusions: This study is of importance to understand the effects of allopurinol in preventing degenerative changes in nerve and glial cells related to spinal cord injuries.en_US
dc.identifier.citationBaloǧlu, M. ve Özkorkmaz, E. G. (2019). Neuroprotective effects of allopurinol on spinal cord injury in rats: A biochemical and immunohistochemical study. Folia Morphologica, 78(4), 676-683.en_US
dc.identifier.doi10.5603/FM.a2019.0036en_US
dc.identifier.endpage683en_US
dc.identifier.issn0015-5659
dc.identifier.issue4en_US
dc.identifier.pmid30949995en_US
dc.identifier.scopus2-s2.0-85077573949en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage676en_US
dc.identifier.urihttps://journals.viamedica.pl/folia_morphologica/article/view/63222
dc.identifier.urihttps://hdl.handle.net/11468/9294
dc.identifier.volume78en_US
dc.identifier.wosWOS:000510490300003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÖzkorkmaz, Ebru Gökalp
dc.language.isoenen_US
dc.publisherVia Medicaen_US
dc.relation.ispartofFolia Morphologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAllopurinolen_US
dc.subjectGlial fibrillary acidic proteinen_US
dc.subjectB-cell lymphoma 2en_US
dc.subjectSpinal cord injuryen_US
dc.subjectRaten_US
dc.titleNeuroprotective effects of allopurinol on spinal cord injury in rats: A biochemical and immunohistochemical studyen_US
dc.typeArticleen_US

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