Comparison of the effects of intratubal injection of adipose-derived mesenchymal stem cells in a rat sciatic nerve transection an experimental study

Basit Öğe Kaydı
dc.authorid0000-0002-0549-8472en_US
dc.contributor.authorKarakol, Perçin
dc.contributor.authorKapı, Emin
dc.contributor.authorKaraöz, Erdal
dc.contributor.authorTünik, Selçuk
dc.contributor.authorBozkurt, Mehmet
dc.date.accessioned2023-03-22T12:32:14Z
dc.date.available2023-03-22T12:32:14Z
dc.date.issued2022en_US
dc.departmentDicle Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Histoloji ve Embriyoloji Ana Bilim Dalıen_US
dc.description.abstractThis study was designed to evaluate the efficacy of epineural tubulization (ENT) with or without intratubal application of adipose-derived mesenchymal stem cells (ASCs) in the rat model of sciatic nerve transection. After formation of 1-cm defect in the left sciatic nerve and ENT, 32 adults female Wistar albino rats were separated into 4 groups (n = 8 for each) including ENT per se (group 1; ENT group) and ENT plus intratubal ASC injection groups killed on day 21 (group 2; ENT-ASC-21-day group), 60 days (group 3; ENT-ASC-60-day group), and 120 days (group 4; ENT-ASC-120-day group). Functional (sciatic function index, hip circumference, withdrawal reflex latency, muscle weight ratio), electrophysiological, histomorphometric, and immunohistochemical analyses were performed in each group. Sciatic function index was significantly higher (-51.98 +/- 5.94, P < 0.01) and withdrawal reflex latency was shorter (-6.21 +/- 2.14, P < 0.01), in the group 4 as compared with all other groups on day 21. Amplitude of contraction was significantly lower in the group 4 as compared with all other groups (0.22 +/- 0.05 vs 0.34 +/- 0.07, 0.50 +/- 0.11, and 0.61 +/- 0.16, P < 0.01 for each). Immunohistochemical analysis revealed presence of green fluorescent protein, vimentin-stained cells, and single neural progenitor cells indicating that induction of neuronal differentiation by ASCs and direct involvement of ASCs within the axonal structure alongside extension of ASCs to the muscular layer of the group 4. In conclusion, our findings revealed that use of ENT plus intratubal ASC injection in a rat sciatic nerve transection model was associated with satisfactory functional outcome and improved peripheral axonal regeneration along with stem cell neural differentiation.en_US
dc.identifier.citationKarakol, P., Kapı, E., Karaöz, E., Tünik, S. ve Bozkurt, M. (2022). Comparison of the effects of intratubal injection of adipose-derived mesenchymal stem cells in a rat sciatic nerve transection an experimental study. Annals of Plastic Surgery, 88(4), 460-466.en_US
dc.identifier.doi10.1097/SAP.0000000000003040
dc.identifier.endpage466en_US
dc.identifier.issn0148-7043
dc.identifier.issn1536-3708
dc.identifier.issue4en_US
dc.identifier.pmid34711729
dc.identifier.scopus2-s2.0-85127730042
dc.identifier.scopusqualityQ2
dc.identifier.startpage460en_US
dc.identifier.urihttps://journals.lww.com/annalsplasticsurgery/Fulltext/2022/04000/Comparison_of_the_Effects_of_Intratubal_Injection.22.aspx
dc.identifier.urihttps://hdl.handle.net/11468/11492
dc.identifier.volume88en_US
dc.identifier.wosWOS:000771079500022
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTünik, Selçuk
dc.language.isoenen_US
dc.publisherLippincott William & Wilkinsen_US
dc.relation.ispartofAnnals of Plastic Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAdipose tissue-derived stem cellsen_US
dc.subjectEpineural tubulizationen_US
dc.subjectPeripheral nerve injuryen_US
dc.subjectRat modelen_US
dc.subjectSciatic nerve transectionen_US
dc.titleComparison of the effects of intratubal injection of adipose-derived mesenchymal stem cells in a rat sciatic nerve transection an experimental studyen_US
dc.titleComparison of the effects of intratubal injection of adipose-derived mesenchymal stem cells in a rat sciatic nerve transection an experimental study
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Comparison_of_the_Effects_of_Intratubal_Injection.22.pdf
Boyut:
706.71 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Makale Dosyası
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Temel Tıp Bilimleri Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu