Cellular distribution of some intermediate filaments in the rat mammary gland during pregnancy, lactation and involution

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dc.contributor.authorBayram, B.
dc.contributor.authorSagsoz, H.
dc.contributor.authorTopaloglu, U.
dc.date.accessioned2025-02-22T14:08:43Z
dc.date.available2025-02-22T14:08:43Z
dc.date.issued2024
dc.departmentDicle Üniversitesien_US
dc.description.abstractIntermediate filaments (IFs) play a major role in determining and maintaining cell shape and anchoring intracellular organelles in place, in the tissues and organs of several species, starting from the early stages of development. This study was aimed at the immunohistochemical investigation of the presence, cellular localization and temporal distribution of the intermediate filaments keratin 8 (CK8), keratin 18 (CK18), keratin 19 (CK19), vimentin, desmin and laminin, all of which contribute to the formation of the cytoskeleton in the rat mammary gland during pregnancy, lactation and involution. On days 7, 14 and 21 of pregnancy (pregnancy period), on day 7 post-delivery (lactation period) and on day 7 post-weaning (involution period), under ketamine hydrochloride (Ketalar-Pfizer) (90 mg/kg) anesthesia, two mammary glands were fully excised from the abdominal region. It was determined that CK8 showed moderate immunoreactions in the alveolar and ductal epithelia, connective tissue and vascular endothelium of the rat mammary gland throughout pregnancy. On the 7 th day of pregnancy, CK18 expression was absent in the alveolar and ductal epithelia, but was observed weakly in some connective tissue cells. Throughout pregnancy, lactation and involution, the alveolar and ductal epithelia of the rat mammary gland were determined to be negative for CK19. Desmin expression predominated in the mammary myoepithelium and vasculature throughout all three of the investigated periods. While vimentin was not expressed in any of the mammary tissue components during pregnancy and lactation, its moderate expression was observed in the alveolar and ductal epithelia during involution. The involution period was also characterized by the vimentin negativity of the myoepithelium, stroma, fat cells and blood vessels of the mammary gland. Throughout all three periods, laminin expression was strong in the alveolar and ductal epithelia, stromal and myoepithelial cells and blood vessels, and did not vary in strength between the investigated periods. These findings demonstrated that intermediate filaments showed cell- and tissue-specific expression patterns in the rat mammary gland under the effects of pregnancy, lactation and involution.en_US
dc.description.sponsorshipScedil;imath;rnak University Scientific Research Projects Coordination Uniten_US
dc.description.sponsorshipWe would like to thank & Scedil;& imath;rnak University Scientific Research Projects Coordination Unit for their support in this study.en_US
dc.identifier.doi10.24425/pjvs.2024.152948
dc.identifier.endpage588en_US
dc.identifier.issn1505-1773
dc.identifier.issn2300-2557
dc.identifier.issue4en_US
dc.identifier.pmid39736032en_US
dc.identifier.scopus2-s2.0-85214188399en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage575en_US
dc.identifier.urihttps://doi.org/10.24425/pjvs.2024.152948
dc.identifier.urihttps://hdl.handle.net/11468/29600
dc.identifier.volume27en_US
dc.identifier.wosWOS:001382076700010
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherPolska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztynen_US
dc.relation.ispartofPolish Journal of Veterinary Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKA_WOS_20250222
dc.subjectcytokeratinen_US
dc.subjectdesminen_US
dc.subjectinvolutionen_US
dc.subjectmammary glanden_US
dc.titleCellular distribution of some intermediate filaments in the rat mammary gland during pregnancy, lactation and involutionen_US
dc.typeArticleen_US

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