Temel Bilimler Bölümü Koleksiyonu

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Three pioneer academics from the field of veterinary surgery in Turkey
(Burdur Mehmet Akif Ersoy University, 2022) Yerlikaya, Nigar; Küçükaslan, Özgül
The first examples of biography studies carried out within the scope of research on the history of veterinary medicine in Turkey were given by Muzaffer Bekman, and these studies were continued with the efforts of Prof. Nihal Erk and Prof. Ferruh Dinçer. These studies, which are also given importance by later academicians in the field of veterinary medicine, are carried out both in the style of necrology and in the style of scientific biography, after the retirement or death of the professionals serving their profession. In this study, the scientific life stories of Ord. Prof. Salih Zeki Berker, Prof. M. Tevfik Başer and Prof. Burhanettin Öktem are discussed. The main material of the study consisted of the personnel files in the Archives of the Dean's Office of the Ankara University Faculty of Veterinary Medicine, and the first-hand sources obtained from the Biography Archive of the History of Veterinary Medicine and Deontology Department. In addition, various books and journals in the Library of the Department were also used. The data obtained were evaluated within the methodology of history and transferred to the text in chronological order. Ord. Prof. Salih Zeki Berker, Prof. M. Tevfik Başer and Prof. Burhanettin Öktem’s academic stages, contributions to education, professional services, publishing activities and the traces they left on their colleagues are revealed. Thus, it is aimed both to contribute to the veterinary medicine biography archive and to introduce these valuable professionals to new generations.
Ankara Üniversitesi Sağlık Bilimleri Enstitüsü Veteriner Hekimliği Temel Bilimler Bölümü lisansüstü tezleri bibliyografyası
(Salim DURUKOĞLU, 2023) Yerlikaya, Nigar; Küçükaslan, Özgül
In this study, the bibliography of the postgraduate theses completed in the veterinary medicine basic sciences department within the 40-year period from the establishment of Ankara University Graduate School of Health Science has been prepared. Theses, which are the main material of the research, were obtained from the archives of Ankara University Graduate School of Health Science, Faculty of Veterinary Dean's office and its units. In addition to the archives of the institution, the National Thesis Center database of the Council of Higher Education was also used. Ankara University Faculty of Veterinary Medicine has departments of anatomy, biochemistry, histology and embryology, history of physiology and veterinary medicine, and deontology. Four of these departments were included in the study, since the thesis data of the history of veterinary medicine and deontology department were previously published by the author, data repetition was not made in this article. While examining the theses, the department, author, name, type, year and advisor information of the thesis were recorded. In the text, the tags of the theses are written in chronological order, and the theses completed in the same year are arranged in alphabetical order. It was determined that a total of 83 master's theses and 159 doctoral theses were completed in four departments of the veterinary medicine basic sciences department between the establishment of the institute (1982) and February 2023. Accordingly, it was determined that the most doctoral theses were completed in the field of veterinary medicine physiology (55). It was determined that the highest number of master's theses were made in the field of biochemistry (54). As a result, it can be argued that this study provides a reference source that will provide convenience to postgraduate students and thesis advisors for their thesis studies planned in the future, and contributes to the bibliographic archive of the history of veterinary medicine.
Ankara Üniversitesi Veteriner Fakültesi Hayvan Yetiştiriciliği ve Sağlık Bilimleri Uzmanlık Yüksekokulunun tarihsel gelişimi
(Salim DURUKOĞLU, 2022) Küçükaslan, Özgül
Ankara Üniversitesi Veteriner Fakültesi Hayvan Yetiştiriciliği ve Sağlık Bilimleri Uzmanlık Yüksek Okulu, 1965 yılında Veteriner Fakültesi Dekanı Prof. Dr. Selahattin Gürtürk döneminde kurulmuştur. Eğitim-öğretim faaliyetlerine 1968 yılında başlanan Uzmanlık Yüksek Okulunda 14 yıl boyunca kesintisiz olarak lisansüstü öğrenci yetiştirilerek veteriner hekimliği mesleğinin gelişimine katkı sağlanmıştır. Yükseköğretim Kanunu’nun yürürlüğe girmesinden sonra lisansüstü eğitimde gerçekleştirilen yeni yapılanma ile birlikte lisansüstü eğitim yeni açılan Sağlık Bilimleri Enstitülerine devredilmiş, bu çerçevede 1982 yılında Uzmanlık Yüksek Okulunun işlevlerine son verilerek Okul kapatılmıştır. Çalışmada, veteriner hekimliği alanında Türkiye’de ilk kez lisansüstü düzeyde krediye dayalı eğitim veren ve tarihsel süreçte veteriner hekimliğe ilişkin bilim alanlarında uzmanlık diploması veren tek kurum haline gelen bu Okulun tarihsel gelişimi ele alınmıştır. Çalışmanın ana materyalini, Ankara Üniversitesi Veteriner Fakültesi Dekanlık Arşivinden sağlanan birinci el kaynaklar oluşturmuştur. Bu kaynaklar arasında kuruma ait orijinal belgeler, diplomalar, faaliyet raporları, rehberler, yönetmelikler, fotoğraflar, uzmanlık ve doktora tezleri bulunmaktadır. Bunun yanı sıra kuruma ait bilgi sahibi olan kişilerin arşivlerinden faydalanılmıştır. Çalışma, kitaplar ve süreli yayınlar gibi ikinci el kaynaklarla desteklenmiştir. Çalışmanın kurgusu “kuruluş ve yapılanma”, “eğitim-öğretim” ve “kapatılma” olmak üzere üç başlık altında düzenlenmiştir. Okulun yapılanması, görev yapmış olan müdürler ve yönetim kurulu üyeleri, lisansüstü eğitim-öğretimin durumu, ders içerikleri, uzmanlık ve doktora eğitimi veren bilim dalları ve öğretim elemanları belirlenmiş, Okuldan mezun olan kişilerden 153’ünün isim ve tez bilgilerine ulaşılmıştır. Elde edilen veriler kronolojik sıra ile yazıya aktarılarak tarih metodolojisi içerisinde değerlendirilmiştir. Sonuç olarak, Ankara Üniversitesi Veteriner Fakültesi Hayvan Yetiştiriciliği ve Sağlık Bilimleri Uzmanlık Yüksek Okulunun tarihsel gelişimine ilişkin ulaşılabilen kaynaklardan sağlanan bilgilerle özelde veteriner hekimliği tarihi çalışmalarına genelde ise kurum tarihi çalışmalarına katkı sağlandığı ileri sürülebilir.
Türk veteriner hekimliği tarihinde aforizmalar
(Salim DURUKOĞLU, 2022) Küçükaslan, Özgül
Aforizma (özdeyiş, özlü söz, vecize, ülger, kelamıkibar, aforizm, motto), bir düşünceyi en kısa ve kesin bir biçimde anlatan ve kimin tarafından söylendiği belli olan söz olarak tanımlanmaktadır. Veteriner hekimliği mesleğine ilişkin olarak büyük devlet adamı İsmet İnönü’nün “Denilebilir ki insan hekimliği veteriner hekimliğin yanında okyanusa karşı iç deniz gibidir” sözleri ve Milli Şair Veteriner Hekim Mehmet Akif Ersoy’un “Bir tecrübe etsen senin aklın da yatar, bize insan hekiminden daha lazım baytar” sözleri, kuşaktan kuşağa geçerek yaşayan ve bu mesleğin her üyesi tarafından bilinen özlü sözlerin başında gelmektedirler. Türkiye’de Cumhuriyet sonrası döneme ilişkin olarak yapılan eser incelemeleri sırasında; deyiş güzelliği, anlatım gücü, kavram zenginliği bakımından araştırma konusu yapılmaya değer bir söz varlığı olduğu tespit edilmiştir. Çalışmada, veteriner hekimliği mesleğinin büyükleri ve devlet adamlarının veteriner hekimliği mesleğine ilişkin olarak dile getirdikleri bu anlamlı sözlerin tarihin tozlu sayfaları arasından çıkarılarak veteriner hekimlerle buluşturulması amaçlanmıştır. Aforizmalar seçilirken, teknik olarak dil bilgisi yönünden aforizma yapısına uygun olmalarına dikkat edilmiş, bu çerçevede; söyleyeni belli, kısa ve yargı bildiren 37 adet aforizma kaydedilmiştir. Çalışmada derli-toplu olarak sunulan bu anlamlı sözlerin meslektaşlarımıza ışık tutacak bir yol göstermeleri bakımından önemli oldukları düşünülmektedir. Aforizmaların, öğrenci derslerinde, meslek yıldönümlerinde, akademik açılış ve mezuniyet törenleri gibi hitabet sanatının ön planda olduğu toplantılarda değerlendirilerek faydalı olabilecekleri ileri sürülebilir.
Türk veteriner hekimliğinde doğum ve jinekoloji alanı lisansüstü tezleri bibliyografyası (1946-2021)
(Salim DURUKOĞLU, 2021) Küçükaslan, Özgül
This bibliography has been conducted in order to create a holistic reference source that will guide researchers who want to work in this field by bringing together the tags of the postgraduate theses completed in this field in the 75-year period from 1946 when the first doctoral thesis in the field of veterinary medicine, obstetrics and gynecology was prepared in Turkey. The material of the study consisted of data obtained from Ankara University Faculty of Veterinary Medicine Dean's Archive, health sciences institutes of universities, and academicians working in the obstetrics and gynecology departments of veterinary faculties. In addition, the data was updated by searching the internet database called "National Thesis Center" of the Council of Higher Education. It has been found that a total of 357 postgraduate theses, of which 143 are master's and 214 are doctoral theses have been completed until May 2021 in the field of veterinary obstetrics and gynecology. It was determined that the highest numbers of postgraduate theses were completed at Ankara University (master's thesis: 27, doctoral thesis: 91). The tags of the doctoral and master's theses were first chronologically ordered according to the year the thesis, and then listed alphabetically. As a result, it can be suggested that the research will contribute to the studies of the history of veterinary medicine in general, and future thesis studies in the field of veterinary obstetrics and gynecology in particular.
Etymological stories
(Salim DURUKOĞLU, 2023) Küçükaslan, Özgül
Etymology is a science which shows the origin of words in a language, investigates when they emerged, where they came from, what stages they went through, deals with the history of both form and meaning of words, examines the origin of a word or word element, when it entered the language, what changes it has undergone. The stories of terms related to medicine/veterinary medicine within the field of etymology are also one of the most interesting topics. It is accepted that storytelling, which benefits students at all educational levels, are powerful tools with significant advantages in the presentation of information content. From this point of view, it is thought that including the word stories reached in the study in the lessons will make the information more meaningful and memorable and reinforce the learning. With the study, it is aimed to bring together people who are interested in the subject with etymological stories and to present a course material to the academicians that can color the students' lessons. For this purpose, the etymological stories of 63 words were obtained. The data obtained are listed as follows: academy, accredited, alkali, ambulance, ammonia, anamnesis, anthrax, arachnoid, achilles tendon, atlas, atom, baitar, baitarname, workhorse, dean, diabetes mellitus, associate professor, doctor, drug, febris, laughing gas, pigeon, hermaphrodite, hygiene, hymen, histology, influenza, iris, campus, kangaroo, cancer, quarantine, kashar cheese, cat, koala, rabies, leopard, logy, morphine, nymphomania, penicillin, percussion, polyclinic, professor, Rp sign in prescriptions, rectum, salami, carp, symposium, senate, cesarean section, rat, sausage, staphylococcus, stethoscope, charlatan, medicine, Turkish saddle, university, wirsung channel, veterinarian, vitamin, yogurt. As a result, it can be argued that it is a resource that can be beneficial for veterinary medicine students to enjoy the lessons, to visualize the information in their minds, to retain the information and thus to learn more easily.
Green-synthesized nanoparticles for biomedical sensor technology
(Elsevier, 2024) Baran, Ayse; Baran, Mehmet Fırat; İpek, Polat; Eftekhari, Aziz; Keskin, Cumali; Atalar, Mehmet Nuri; Alma, Mehmet Hakkı
Sensor technology is an integral part of the many cost-effective and efficient factors possible in modern medical devices. Biosensors have good potential as they are easy, scalable, and effective in manufacturing processes. Nanotechnology has become one of the promising technologies applied in all fields of science. Biotechnologically produced metallic nanoparticles (NP) attract attention in scientific applications and technology platforms due to their extensive applications in biomedical and physiochemical fields. In recent years, the side effects caused by the use of synthetic drugs and the medical and economic problems caused by them have made the use of plants popular again. Ecofriendly, nontoxic metal-based NPs (such as gold, silver, palladium, manganese, and zinc) smaller than 100nm in size can be synthesized with extracts obtained from plants by different methods. As the sizes and shapes of NPs change, the physical, chemical, bioactive, optical, electrical, catalytic, and toxicity properties of the particles also change. Biological synthesis, also known as green synthesis, is a practical method to obtain NPs easily and ecologically without the need for high pressure, high-temperature values, and toxic chemicals. Green synthesis of NPs is carried out using different biomaterials such as bacteria, fungi, yeast, viruses, microalgae, and plant biomass/essence. Plant-mediated biosynthesis of metallic NPs occurs through biomolecules containing organic functional groups in the plant. Nanobiosensors, analytical devices combining a biologically sensitive element with a nanostructured transducer, are widely used for the molecular detection of biomarkers associated with the diagnosis of diseases and the detection of infectious organisms. Nanobiosensors show certain advantages over laboratory and many field methods due to their inherent specificity, simplicity, and rapid response. In this study, advancements in the development of nanobiosensors are illuminated. Considering all these aspects, it can be said that nanobiosensors enable diagnostic tools with increased sensitivity, specificity, and reliability for medical applications. © 2025 Elsevier Inc. All rights reserved.
Cellular distribution of some intermediate filaments in the rat mammary gland during pregnancy, lactation and involution
(Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2024) Bayram, Bayram; Sağsöz, Hakan; Topaloğlu, Uğur
Intermediate filaments (IFs) play a major role in determining and maintaining cell shape and anchoring intracellular organelles in place, in the tissues and organs of several species, starting from the early stages of development. This study was aimed at the immunohistochemical investigation of the presence, cellular localization and temporal distribution of the intermediate filaments keratin 8 (CK8), keratin 18 (CK18), keratin 19 (CK19), vimentin, desmin and laminin, all of which contribute to the formation of the cytoskeleton in the rat mammary gland during pregnancy, lactation and involution. On days 7, 14 and 21 of pregnancy (pregnancy period), on day 7 post-delivery (lactation period) and on day 7 post-weaning (involution period), under ketamine hydrochloride (Ketalar-Pfizer) (90 mg/kg) anesthesia, two mammary glands were fully excised from the abdominal region. It was determined that CK8 showed moderate immunoreactions in the alveolar and ductal epithelia, connective tissue and vascular endothelium of the rat mammary gland throughout pregnancy. On the 7 th day of pregnancy, CK18 expression was absent in the alveolar and ductal epithelia, but was observed weakly in some connective tissue cells. Throughout pregnancy, lactation and involution, the alveolar and ductal epithelia of the rat mammary gland were determined to be negative for CK19. Desmin expression predominated in the mammary myoepithelium and vasculature throughout all three of the investigated periods. While vimentin was not expressed in any of the mammary tissue components during pregnancy and lactation, its moderate expression was observed in the alveolar and ductal epithelia during involution. The involution period was also characterized by the vimentin negativity of the myoepithelium, stroma, fat cells and blood vessels of the mammary gland. Throughout all three periods, laminin expression was strong in the alveolar and ductal epithelia, stromal and myoepithelial cells and blood vessels, and did not vary in strength between the investigated periods. These findings demonstrated that intermediate filaments showed cell- and tissue-specific expression patterns in the rat mammary gland under the effects of pregnancy, lactation and involution.
Antioxidant properties of allium turcicum Özhatay & cowley plant extract, its effects on the proliferation and migration of cancer cells
(Frontiers Media SA, 2024) İpek, Polat; Baran, Ayşe; Cebe, Deniz Barış; Ahmadian, Elham; Eftekhari, Aziz; Baran, Mehmet Fırat
Cancer is a type of non-communicable disease that is responsible for numerous deaths worldwide. Cancer incidence and mortality rates are on the rise due to a combination of factors, such as a growing population, aging, and poor dietary habits. The Allium turcicum Özhatay & Cowley plant is an endemic plant in the area where it grows and is consumed by the public due to its various benefits. This endemic plant, which generally grows in high-altitude regions, is sold in bunches because it is costly, mixed with rock salt, crushed into powder, and consumed as a spice. The cytotoxic and growth-inhibitory effects of A. turcicum Özhatay & Cowley herb extract on human glioblastoma U373 cells, human colorectal carcinoma cell HCT-116, and healthy HUVEC cell lines were determined by the MTT method. After 24 and 48 h of application, logIC50 values in HUVEC, HCT-116, and U373 cells were defined as 3.737, 3.765; 3.513, 3.696, 4.476, and 4.104 μg/mL, respectively. We conducted a cell migration experiment to study the A. turcicum Özhatay & Cowley Extract (ATÖCE) impact on cancer cells’ metastatic behavior. Our findings indicate that ATÖCE has an inhibitory effect on the migration potential of the cells used in the study. We conducted experiments using DPPH, ABTS, CUPRAC, and total phenolic content to assess the antioxidant properties of ATÖCE. The findings from the antioxidant activity experiments revealed an activity level of 0.20 ± 0.046 at IC50. Additionally, the total phenolic content was measured to be 0.26 ± 0.044 mg GAE/g. Copyright © 2024 İPEK, Baran, Cebe, Ahmadian, Eftekhari and Baran.
Deciphering the effect of Potentilla fulgens root extract against healthy HUVEC cell line and cancer cell lines (A549 and SKOV-3)
(Elsevier B.V., 2024) İpek, Polat; Baran, Ayşe; Baran, Mehmet Fırat; Eftekhari, Aziz; Khusro, Ameer; Ommati, Mohammad Mehdi; Aliyev, Elvin; Khalilov, Rovshan
Background: Potentilla fulgens, a highly valued indigenous medicinal herb grown in high altitudes of the Himalayan region with anticancer, hypoglycaemic, antibacterial, anti-inflammatory, and antiulcerogenic properties, are used in traditional systems of medicine. This study was aimed to investigate the effect of P. fulgens root extract, as one of the natural alternatives to chemotherapeutic drugs used in cancer treatment, on proliferation, apoptosis, and autophagy of human non-small cell lung cancer cell line (A549), human ovarian cancer cell line (SKOV-3), and healthy human umbilical vein endothelial cell line (HUVEC). Methods: Anti-proliferative effect was assessed by MTT assay. The expression of autophagy and apoptosis-related proteins was evaluated by western blotting. Total oxidant status (TOS) and total antioxidant capacity (TAC) test were determined using standard kit methods. Results: Our results showed that the extract inhibited proliferation of HUVEC, A549, and SKOV-3 cells in a dose-dependent manner. MTT assay analysis revealed that the extract significantly (P<0.05) induced mortality in HUVEC, A549, and SKOV-3 cells. Western blot results revealed increased expression of NF-κB after the extract treatment but led to the down-regulation in Beclin-1, Bax, extracellular-signal-related kinase 1 and 2, Sequestosome-1, and cleaved Casp-3 levels. Treatment groups showed an increase in TOS and TAC values in A549 and SKOV-3 cell lines, while HUVEC cell line showed an increase in TAC and a decrease in TOS values, compared to the control group. Conclusions: Our findings indicated that P. fulgens root extract inhibited the proliferation of healthy cells and cancer cells through cell cycle arrest, representing its limited application as therapeutic agent in cancer treatment.
Integrative studies of Microtus hartingi and Microtus guentheri (Cricetidae, Arvicolinae) and taxonomic problem of Microtus elbeyli
(Elsevier GmbH, 2024) Selçuk, Ahmet Yesari; Kaya, Alaettin; Kefeli̇oğlu, Haluk
In this study, Taxonomic differences between Microtus guentheri and Microtus hartingi, which are similar in terms of external morphological features, were determined using linear morphometric, geometric morphometric, karyological and molecular analyses methods. Through classical morphometric (mean values) and geometric morphometric analyses of craniodental and mandible characters, influenced by various genetic, developmental, and functional processes, it was found that M. hartingi and M. guentheri exhibited notable differences in both mean linear measurements and shape. The Jackknife in classical morphometric analyses of samples, which underwent molecular and karyological species diagnoses, was higher than 88 % for each character. In geometric morphometric analyses, the Procrustes distance for shape follows the order Ventral < Dorsal < Mandible < M3
The expression of intermediate filaments in the abomasum of ruminants: A comparative study
(John Wiley and Sons Inc., 2024) Aydın, Nurşin; Ketani, Muzaffer Aydın; Sağsöz, Hakan
Intermediate filaments (IFs) are key molecular factors of the cell and have been reported to play an important role in maintaining the structural integrity and functionality of the abomasum. This study was designed to determine the regional distribution, cellular localization and expression of several IFs, including CK8, CK18, CK19, vimentin, desmin, peripherin and nestin, as well as the connective tissue component laminin, in the bovine, ovine and caprine abomasa. Immunohistochemical analyses demonstrated varying levels of expression of CK8, CK18, CK19, vimentin, desmin, nestin, peripherin and laminin in the bovine, ovine and caprine abomasa. CK8 immunoreactions were particularly evident in the luminal and glandular epithelia of the glands found in the abomasal cardia, fundus and pylorus in all three species. In the bovine abomasum, CK18 immunoreactions were stronger in the parietal cells, compared to the chief cells. In the abomasum of all three species, the smooth muscle as well as the smooth muscle cells of the vascular media in the cardiac, fundic and pyloric regions showed strong immunoreactivity. In all three species, the cardiac, fundic and pyloric regions of the abomasum showed strong peripherin and nestin immunoreactions in the luminal and glandular epithelial cells, stromal and smooth muscle cells, nervous plexuses and blood vessels. The expression patterns of IFs and laminin in the ruminant abomasum suggest that these proteins play a structural role in the cytoskeleton and are effective in maintaining abomasal tissue integrity and stability.
Variation in the shape and size of the scale of the Tigris bream (Acanthobrama marmid, Heckel, 1843) from the Tigris River, Türkiye attributed to Seasonality, Age and Sex: A geometric morphometric study
(Universidad del Zulia, Facultad de Ciencias Veterinarias, 2024) Bilici, Serbest; Kaya, Alaettin; Dörtbudak, Muhammed Yaşar; Çiçek, Tarık; Ünlü, Erhan
In this study, the Tigris bream Acanthobrama marmid individuals (44 females and 31 males) were captured from the Tigris River. The scale size (as centroid size) and shape were analyzed separately using 2– dimensional geometric morphometric methods. Procrustes ANOVA revealed significant differences in scales size between sexes, while no difference in shape was observed. Groups based on season and age showed significant differences in both size and shape. Female individuals had larger scale sizes than males, with the scales of the Autumn group being larger than those of the Spring and Summer groups. Scale size also increased with age groups. PCA analysis showed variation in the first five components when examined by age, season, and gender. CVA and DFA results indicated significant differences in shape between different age groups and seasonal groups, but no significant differences between sexes were observed.
Prognostic significance of the chemerin level in coronavirus disease 2019 patients
(Lippincott Williams and Wilkins, 2024) Gökdemir, Gül Şahika; Gökdemir, Mehmet Tahir; Araç, Songül; Yokuş, Beran
Increased serum chemerin levels have been reported in several inflammatory diseases. Few studies have investigated the relationship between chemerin and clinical features of COVID-19. Thus, chemerin may modulate the development and progression of COVID-19. We compared the serum chemerin concentration between patients with and without SARS-CoV-2 infection and its association with the severity and prognosis of COVID-19 pneumonia. This is a prospective, single-center, cross-sectional study. We enrolled COVID-19 patients who presented to our tertiary hospital and healthy controls. The COVID-19 patients were conducted and the dates of symptom onset were recorded. After admission to the hospital and stabilization, blood samples were obtained for routine hemogram, biochemistry, and chemerin. The chemerin level was 37.93?±?17.3 ng/mL in patients followed in the ICU, 29.41?±?12.79 ng/mL in inpatients, 30.48?±?10.86 ng/mL in outpatients, and 25.12?±?9.82 ng/mL in healthy controls. The difference between patients treated in the ICU and healthy controls was significant (P?
Determination of chemical components of the endemic species Allium turcicum L. plant extract by LC-MS/MS and evaluation of medicinal potentials
(Elsevier Ltd, 2024) İpek, Polat; Atalar, Mehmet Nuri; Baran, Ayşe; Baran, Mehmet Fırat; Ommati, Mohammad Mehdi; Karadağ, Musa; Zor, Murat
The Allium turcicum L. (Zuzubak) plant as a cultivated vegetable have various health benefits and consumed as a food. Due to the shortcoming evidence in literature and the importance of this plant in folk medicine, in the present study, for the first time, we evaluated the bioactive profile of components (using LC-MS/MS), cytotoxicity, anticancer, antioxidant, and antibacterial prospectives of Zuzubak methanol extract. Reported results show that the extract is rich in bioactive compounds and has anticancer activity with breast cancer cells (MCF-7), human prostate cancer cells (DU-145), and Human osteosarcoma cancer Cell lines of (IC50) in dose dependent manner in the concentration range of 31.25 ?g/mL and 2000 ?g/mL for 24 and 48 h. Western blotting results determined that the extract significantly suppressed the growth of U2OS, MCF-7, and DU-145 cancer cells by down expression of Ang-1 (angiogenic protein) and Beclin-1 (autophagy protein) and overexpression of Bax (a proapoptotic protein). The oxidative stress indices showed a reduction in RPE-1 and MCF-7 cells and an upsurge in U2OS and DU-145 cells. Additionally, the antimicrobial assay showed suppression of the growth of various pathogenic microorganisms in 4.00–8.00 ?g/concentrations of Zuzubak extract using the microdilution method. The phytochemicals identified showed promising anticancer, antioxidant effects, and antimicrobial properties, representing a valuable herbal source for drug development studies. © 2024 The Authors
Composition of abomasal mucins in hair goats
(Taylor & Francis Ltd, 2021) Karakoç, Zelal; Topaloğlu, Uğur; Ketani, Muzaffer Aydin
The mucosal epithelium of the digestive tract is covered by a layer of mucusthat protects against chemical, enzymatic and mechanical damage, and pathogenic microorganisms. We investigated the composition of the mucin in the abomasum of goat kids using histochemistry and immunohistochemistry. We used 6 - 8-month-old goat kids; samples were collected from the cardia, fundus and pylorus regions of the abomasum. Alcian blue (pH.2.5)-PAS, Alcian blue (pH.2.5)-aldehyde fuchsin, phenylhydrazine-PAS histochemical staining methods were used as well as Muc1, Muc2, Muc5ac and Muc6 immunohistochemistry. We found that neutral mucins, carboxylic mucins and N-acetyl sialomucins were present in the stomachs of the kids. Muc1 was expressed in all glandular epithelium cells and was expressed intensely by the mucous neck cells in the fundus. Although Muc2 expression was not observed in the cardia and fundus, weak expression was found in the surface epithelial cells and mucus secreting cells of the pylorus. Muc5ac was expressed intensely in all glandular epithelial cells and in mucous neck cells of the fundus as well as mucus secreting cells in the pylorus. Moderately intense Muc6 expression also was found in the cardia and fundus; it was expressed intensely in the mucus secreting cells of the pylorus.
Gross anatomy and arterial vascularization of the tympanic cavity and osseous labyrinth in mid-gestational bovine fetuses
(Wiley-Liss, 2010) Erdoğan, Serkan; Kılınç, Mehmet; 0000-0002-5013-9540; 0000-0001-8853-9657
This study aims to determine morphological features of certain aural formations, varietal characteristics, and arterial supply in fetal development period in cattle. For this purpose, ears of 10 bovine fetuses in mid-gestation were evaluated. Organ morphology and vascularization in prenatal life were investigated by using corrosion cast technique. It was observed that some aspects of osseous formation and vascular organization in middle and inner ears were not developed completely in the first half of gestation; in addition, cochlea did not its snail-like structure yet, lateral semicircular canal was rather low compared to others and auditory ossicles did not take its final shape. The feeding blood vessels of inner ear were found to demonstrate three different distribution patterns, whereas feeding pattern in middle ear was very similar in many specimens. The presence of stapedial artery was also identified. Differences and similarities with other some species were assessed in terms of both general morphological structure and vascular organization. From this regard, it is thought that this study will constitute a comparative model for both humans and other species and provide morphological contributions since there is not sufficient literature on species-specific ear morphology in the field of veterinary anatomy in contrast to the abundance of studies on humans. Anat Rec, 293:2083-2093, 2010. (C) 2010 Wiley-Liss, Inc.
The protective effect of Pomegranate extract against the experimental gastric ulcer induced by ethanol in rats
(Universidad del Zulia, Facultad de Ciencias Veterinarias, 2024) Karakoç, Zelal; Oruç, İdris; Toptancı, Bircan Çeken; Baksi, Nazan; Ketani, Muzaffer Aydın
The Pomegranate (Punica granatum) is a commonly found fruit in the Mediterranean and Iran, which has a variety of uses including medicinal purposes, cosmetics, and as a spice in culinary applications. Pharmacological functions of Pomegranate include antioxidation, anti–tumor, anti–hepatotoxicity, anti–lipoperoxidation and antibacterial properties. The aim of this study was to evaluate the therapeutic efficacy of Pomegranate extract by utilizing its antioxidant activity in an experimental rat model of gastritis induced by ethanol. In the study, 24 female Wistar albino rats (180–200 g) were used. Gastritis in rats was induced using Ethanol. In experimental groups, Tumor necrosis factor–alpha, Myloperoxidase, Superoxide Dismutase and Malondialdehyde were examined for biochemical analyzes. Streptavidin peroxidase immunohistochemistry method was applied to gastric tissues with gastritis. A statistically significant difference was observed between Superoxide Dismutase and Meloperoxidase levels. CD8 and CD68 immunoreactivity was higher in the Ethanol group compared to the other groups. A decrease was observed in CD8 and CD68 positive immunoreactivity in Ethanol+Pomegranate extract group compared to Ethanol group. The study found that the immunoreactivity of MHC–I and MHC–II was found in specific locations, namely intraepithelial lymphocytes located in the epithelium, some capillary vessel endothelium, and connective tissue. Changes in anti–oxidative stress markers such as Superoxide Dismutase and Myloperoxidase contributed to the mucosal protective effect of Pomegranate extract in Ethanol–induced gastritis.
Effect of vitamin C at different doses on the oxidant/antioxidant system of ewes during late pregnancy, early and late lactation and their lambs
(Agricultural Research Communication Centre, 2024) Gürgöze, Sema; Alak, İlyas; Durak, Mehmet Hanifi
Background: The development of connective tissue in the fetus during the ruminant gestation period increases the need for vitamin C, which is then further increased by the course of pregnancy and lactation. As pregnancy and lactation develop, free radicals and MDA produced as a result of oxidative stress interact with functional groups in the cell, resulting in cell damage. The oxidant/antioxidant balance at this time can be calculated using the total antioxidant status, total oxidant status and oxidative status index values. This study aimed to investigate the effect of two different doses of vitamin C on oxidative stress indicators in late gestation, early and late lactation ewes and their offspring. Methods: In the study, 48 ewes of the German Meat Merino and Akkaraman breeds that were developed by crossbreeding were employed. After breeding, four groups were formed from pregnant and non-pregnant ewes. Negative Pregnancy Control group was formed from non-pregnant ewes. Positive Pregnancy Control, Positive Pregnancy Practice 1 and Positive Pregnancy Practice 2 groups were formed from pregnant ewes. After the 90th day of pregnancy, ewes in the Negative Pregnancy Control and Positive Pregnancy Control groups received saline (0.9% NaCl, 2.5 ml) injections every week concurrently with the other pregnant groups until delivery. From the 90th day of pregnancy until birth, sheep participating in Positive Pregnancy Practices 1 and 2 received weekly injections of vitamin C (625 mg/CA, 2.5 ml and 1250 mg/CA, 5 ml, respectively). Result: In the study, no statistically significant difference was found in serum MDA, TAS, TOS and OSI levels between late pregnancy, lactation and born lambs. While none of the pregnant ewes in this study aborted, vitamin C injection significantly decreased postnatal lamb mortality. This showed that vitamin C helped pregnant ewes feel less stressed throughout pregnancy and helped lower postnatal lamb mortality. In pregnant ewes, AO-effective vitamin C supplementation has been shown to have possible beneficial effects on both maternal and offspring health, whereby the number of stillbirths was reduced by vitamin C supplementation, especially in Positive Pregnancy Practice 2 lambs.
Investigation of serum trace element levels in sheep in Diyarbakır Province and districts
(Agricultural Research Communication Centre, 2024) Durak, Mehmet Hanifi; Gürsel, Feraye Esen; Akış, Iraz; Gürgöze, Sema
Background: In this study, the serum of sheep raised on pasture in the province and districts of Diyarbakır was tested for concentrations of the essential trace elements Cr, Cu, Se, Zn, Co, Mn, ultra-trace elements like Ni, V and Ag and non-essential toxic trace elements like Al, Pb, As, Cd and Ti. Methods: The animals used in the study consisted of 313 healthy sheep at the end of lactation obtained from different districts of Diyarbakır. The closed system method of wet decomposition was used to transfer serum trace elements to the soluble phase in blood samples. Serum trace elements were analyzed using an ICP-MS device. Result: This study contributed to obtaining normal levels of Al, Ag, Ni, Ti and V trace elements in ewes at the end of lactation. It was observed that there was no difference between all districts in terms of serum Al, Ni, Cu and Zn trace element levels. In the study, differences were found in the serum Cr, Se, As and Cd trace element levels of sheep between the districts; however, it was determined that no significant deficiency or excess occurred and these levels were within normal limits. The Co and Mn levels we obtained in Diyarbakır are much lower than the average levels reported by the researchers. We can say that the addition of Co and Mn trace elements in appropriate proportions to the rations of animals can positively affect animal health and productivity.

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