Carboligation reactions mediated by benzoylformate decarboxylase immobilized on a magnetic solid support

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dc.contributor.authorTural, Bilsen
dc.contributor.authorTural, Servet
dc.contributor.authorDemir, Ayhan S.
dc.contributor.orcid0000-0001-7555-2481
dc.contributor.orcid0000-0003-2479-9438
dc.date.accessioned2024-04-24T15:59:22Z
dc.date.available2024-04-24T15:59:22Z
dc.date.issued2013
dc.departmentDicle Üniversitesi, Ziya Gökalp Eğitim Fakültesi, Matematik ve Fen Bilimleri Eğitimi Bölümüen_US
dc.description.abstractIn this study, magnetic nanoparticles (Fe3O4, magnetite) with immobilized metal affinity ligands (MSS) were prepared and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), and vibrating-sample magnetometer (VSM) methods for purification and immobilization of the histidine-tagged recombinant benzoylformate decarboxylase (BFD). The MSS support was shown to be eligible for selective binding of HIS-tagged BFD by SDS-page analysis. Loading capacity of the MSS support was determined as 43.6 +/- 1.1mg/g. The regeneration ability for protein binding was also studied. An immobilized BFD was tested to catalyze benzoin condensation and representative cross acyloin reaction. Conversion and enantiomeric excess values were comparable with that of free enzyme catalyzed reactions. (C) 2013 Wiley Periodicals, Inc.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [109T497]en_US
dc.description.sponsorshipContract grant sponsor: Scientific and Technological Research Council of Turkey; Contract grant number: TUBITAK, 109T497.en_US
dc.identifier.citationTural, B., Tural, S. ve Demir, A. S. (2013). Carboligation reactions mediated by benzoylformate decarboxylase immobilized on a magnetic solid support. Chirality, 25(7), 415-421.
dc.identifier.doi10.1002/chir.22181
dc.identifier.endpage421en_US
dc.identifier.issn0899-0042
dc.identifier.issn1520-636X
dc.identifier.issue7en_US
dc.identifier.pmid23728901
dc.identifier.scopus2-s2.0-84879419880
dc.identifier.scopusqualityQ2
dc.identifier.startpage415en_US
dc.identifier.urihttps://doi.org/10.1002/chir.22181
dc.identifier.urihttps://hdl.handle.net/11468/14013
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/10.1002/chir.22181
dc.identifier.volume25en_US
dc.identifier.wosWOS:000325921200007
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorTural, Bilsen
dc.institutionauthorTural, Servet
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofChirality
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMetal-affinity magnetic nanoparticlesen_US
dc.subjectHis-tagged protein purificationen_US
dc.subjectBenzoylformate Decarboxylase (Bfd)en_US
dc.subjectCarboligation reactionsen_US
dc.titleCarboligation reactions mediated by benzoylformate decarboxylase immobilized on a magnetic solid supporten_US
dc.titleCarboligation reactions mediated by benzoylformate decarboxylase immobilized on a magnetic solid support
dc.typeArticleen_US

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