Carboligation reactions mediated by benzoylformate decarboxylase immobilized on a magnetic solid support
Yükleniyor...
Tarih
2013
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, magnetic nanoparticles (Fe3O4, magnetite) with immobilized metal affinity ligands (MSS) were prepared and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), and vibrating-sample magnetometer (VSM) methods for purification and immobilization of the histidine-tagged recombinant benzoylformate decarboxylase (BFD). The MSS support was shown to be eligible for selective binding of HIS-tagged BFD by SDS-page analysis. Loading capacity of the MSS support was determined as 43.6 +/- 1.1mg/g. The regeneration ability for protein binding was also studied. An immobilized BFD was tested to catalyze benzoin condensation and representative cross acyloin reaction. Conversion and enantiomeric excess values were comparable with that of free enzyme catalyzed reactions. (C) 2013 Wiley Periodicals, Inc.
Açıklama
Anahtar Kelimeler
Metal-affinity magnetic nanoparticles, His-tagged protein purification, Benzoylformate Decarboxylase (Bfd), Carboligation reactions
Kaynak
Chirality
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
25
Sayı
7
Künye
Tural, B., Tural, S. ve Demir, A. S. (2013). Carboligation reactions mediated by benzoylformate decarboxylase immobilized on a magnetic solid support. Chirality, 25(7), 415-421.
