Melatonin prevents nicotine-induced hepatotoxicity by modulating apoptosis and histopathological changes in rats

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dc.contributor.authorSengul, S. A.
dc.contributor.authorTaskin, I. Icen
dc.contributor.authorAsir, F.
dc.contributor.authorSakar, A. Eraslan
dc.contributor.authorSengul, G. Pektanc
dc.date.accessioned2025-02-22T14:08:43Z
dc.date.available2025-02-22T14:08:43Z
dc.date.issued2024
dc.departmentDicle Üniversitesien_US
dc.description.abstractNicotine, the main toxic component of tobacco, directly or indirectly causes adverse effects on the liver metabolism. Melatonin, secreted by the pineal gland, has anti-apoptotic activity as well as antioxidant activity. The aim of this study was to reveal the antiapoptotic effects of melatonin in rats with experimentally induced chronic liver damage with nicotine. In this study, 32 male Wistar albino rats were divided into four groups: control, melatonin, nicotine and nicotine+melatonin. During the experiment, nicotine (1 mg/kg) and melatonin (10 mg/kg) were administered daily intraperitoneally for 56 days. At the end of the study, the liver tissues were taken for histopathological, immunohistochemical and molecular analysis. The administration of melatonin was determined to partially alleviate histopathological changes in the liver tissue induced by nicotine, such as hepatocyte degeneration, vascular dilatation and congestion, and leukocyte infiltration. It was observed that there was a significant decrease in Bax expression levels and a significant increase in Bcl-2 expression levels in the nicotine+melatonin group when compared to the injury group. On the other hand, it was determined that melatonin administration reduced the Bax/Bcl-2 ratio, which was significantly higher in the nicotine group compared to the other groups, to a level close to the control group. Additionally, as a result of immunohistochemical evaluation, it was observed that decreased Bax expression and increased Bcl-2 expression in hepatocytes in the nicotine+melatonin group were at a level close to the control group. Our results revealed that melatonin is a hepatoprotective and effective antioxidant by suppressing cell apoptosis and increasing the rate of healing after damage at both the immunohistochemical and molecular levels.en_US
dc.identifier.doi10.24425/pjvs.2024.152949
dc.identifier.endpage597en_US
dc.identifier.issn1505-1773
dc.identifier.issn2300-2557
dc.identifier.issue4en_US
dc.identifier.pmid39736033en_US
dc.identifier.scopus2-s2.0-85214186736en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage589en_US
dc.identifier.urihttps://doi.org/10.24425/pjvs.2024.152949
dc.identifier.urihttps://hdl.handle.net/11468/29599
dc.identifier.volume27en_US
dc.identifier.wosWOS:001382076700011
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherPolska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztynen_US
dc.relation.ispartofPolish Journal of Veterinary Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKA_WOS_20250222
dc.subjectapoptosisen_US
dc.subjecthepatotoxicityen_US
dc.subjectimmunohistochemistryen_US
dc.subjectmelatoninen_US
dc.subjectnicotineen_US
dc.subjectprotein expressionen_US
dc.titleMelatonin prevents nicotine-induced hepatotoxicity by modulating apoptosis and histopathological changes in ratsen_US
dc.typeArticleen_US

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