Absence of the mecC gene in methicillin-resistant Staphylococcus aureus isolated from various clinical samples: The first multi-centered study in Turkey

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dc.authorid0000-0003-3475-660Xen_US
dc.contributor.authorÇıkman, Aytekin
dc.contributor.authorAydın, Merve
dc.contributor.authorGülhan, Barış
dc.contributor.authorKarakeçili, Faruk
dc.contributor.authorKurtoǧlu, Muhammet Güzel
dc.contributor.authorYüksekkaya, Şerife
dc.contributor.authorParlak, Mehmet
dc.contributor.authorGültepe, Bilge Sümbül
dc.contributor.authorÇiçek, Ayşegül Çopur
dc.contributor.authorBilman, Fulya Bayındır
dc.contributor.authorÇİftçİ, İhsan Hakkı
dc.contributor.authorKara, Murat
dc.contributor.authorAtmaca, Selahattin
dc.contributor.authorÖzekinci, Tuncer
dc.date.accessioned2021-09-14T06:14:37Z
dc.date.available2021-09-14T06:14:37Z
dc.date.issued2019en_US
dc.departmentDicle Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalıen_US
dc.descriptionPMID:30745200
dc.description.abstractBackground: mecA is a predefined gene causing methicillin resistance in Staphylococcus aureus (S. aureus) isolates; however, it has been shown that some methicillin-resistant S. aureus (MRSA) strains do not carry this gene. Recently, in isolates found to be MRSA-positive but mecA-negative, a new resistance gene called mecC, which is a homolog of mecA, has been reported. This study aimed to investigate the mecC and mecA genes in MRSA strains isolated from different geographic regions in Turkey. Methods: The sample of the study consisted of 494 MRSA strains isolated from seven geographical regions in Turkey between 2013 and 2016. The strains were obtained from 17 centers, comprising 13 university hospitals, three education and research hospitals, and one state hospital. Methicillin resistance in S. aureus strains was determined using the agar disk diffusion method with a cefoxitin disk and the agar dilution method with oxacillin. The mecC and mecA genes in MRSA strains was investigated by Polymerase Chain Reaction (PCR). Results: Of the MRSA strains investigated, 47.9% were isolated from intensive care units. Concerning sample type, 36.7% were detected in the respiratory tract (tracheal aspirate, sputum, etc.), 24.8% in blood, 18.7% in skin and soft tissues, 9.3% in nasal swabs, 5.4% in urine, 4.1% in ears, and 1% in sterile body fluid. Using PCR, mecC was not identified in any of the S. aureus strains isolated from different clinical microbiology laboratories. mecA gene positivity was found in 315 of the MRSA strains (63.8%). Staphylococcal Cassette Chromosome mec (SCCmec) type was identified in 232 strains (46.9%), of which 136 (58.7%) were type II, 75 (32.4%) were type IV, 12 (5.1%) were type IIIb, six (2.5%) were type I, and three (1.3%) were type III. Conclusion: This is the first multi-centered study to investigate MRSA strains isolated from different regions in Turkey. The mecC gene was not detected in any of the MRSA strains. We believe that this study will constitute an important basis for monitoring possible future changes.en_US
dc.identifier.citationÇıkman, A., Aydın, M., Gülhan, B., Karakeçili, F., Kurtoǧlu, M. G., Yüksekkaya, Ş. ve diğerleri. (2019). Absence of the mecC gene in methicillin-resistant Staphylococcus aureus isolated from various clinical samples: The first multi-centered study in Turkey, Journal of Infection and Public Health, 12(4), 528-533.en_US
dc.identifier.doi10.1016/j.jiph.2019.01.063
dc.identifier.endpage533en_US
dc.identifier.issn1876-0341
dc.identifier.issue4en_US
dc.identifier.pmid30745200
dc.identifier.scopus2-s2.0-85061095396
dc.identifier.scopusqualityQ1
dc.identifier.startpage528en_US
dc.identifier.urihttps://www.clinicalkey.com/#!/content/playContent/1-s2.0-S1876034119300656?returnurl=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1876034119300656%3Fshowall%3Dtrue&referrer=https%3A%2F%2Fwww.scopus.com%2Frecord%2Fdisplay.uri%3Feid%3D2-s2.0-85061095396%26origin%3Dresultslist%26sort%3Dplf-f%26src%3Ds%26nlo%3D1%26nlr%3D20%26nls%3Dafprfnm-t%26affilName%3Ddicle%2Buniv*%26sid%3Db3b80e7d29688bfa9a24aae85e69f494%26sot%3Dafnl%26sdt%3Dcl%26cluster%3Dscofreetoread%252c%2522all%2522%252ct%252bscopubyr%252c%25222019%2522%252ct%26sl%3D38%26s%3D%2528AF-ID%2528%2522Dicle%2B%25c3%259cniversitesi%2522%2B60016299%2529%2529%26relpos%3D18%26citeCnt%3D8%26searchTerm%3D
dc.identifier.urihttps://hdl.handle.net/11468/7538
dc.identifier.volume12en_US
dc.identifier.wosWOS:000473414800014
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzekinci, Tuncer
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofJournal of Infection and Public Health
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectmecAen_US
dc.subjectmecCen_US
dc.subjectMethicillin resistanceen_US
dc.subjectStaphylococcus aureusen_US
dc.subjectTurkeyen_US
dc.titleAbsence of the mecC gene in methicillin-resistant Staphylococcus aureus isolated from various clinical samples: The first multi-centered study in Turkeyen_US
dc.titleAbsence of the mecC gene in methicillin-resistant Staphylococcus aureus isolated from various clinical samples: The first multi-centered study in Turkey
dc.typeArticleen_US

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