Newly developed peptide-ELISA successfully detected anti-IgG antibodies against Maedi-Visna virus in sheep

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dc.authorid0000-0001-9633-9786en_US
dc.authorid0000-0003-2705-2831en_US
dc.authorid0000-0001-7378-6962en_US
dc.authorid0000-0002-2468-5564en_US
dc.authorid0000-0002-0711-0750en_US
dc.contributor.authorKoçkaya, Ecem Su
dc.contributor.authorCan, Hüseyin
dc.contributor.authorYaman, Yalçın
dc.contributor.authorKandemir, Çağrı
dc.contributor.authorTaşkın, Turgay
dc.contributor.authorKarakavuk, Muhammet
dc.contributor.authorŞireli, Halit Deniz
dc.date.accessioned2024-08-15T10:33:57Z
dc.date.available2024-08-15T10:33:57Z
dc.date.issued2024en_US
dc.departmentDicle Üniversitesi, Ziraat Fakültesi, Zootekni Bölümüen_US
dc.description.abstractMaedi Visna Virus (MVV) is a retrovirus that can infect sheep. There is still no effective therapy or vaccine against this virus and timely diagnosis is important to combat the complications of the disease. In this study, we aimed to develop an ELISA using peptides derived from gag protein as antigen. For this purpose, B cell epitopes of gag protein were predicted and a docking analysis with the B cell receptor was performed to select peptides to be used in ELISA. After three soluble epitopes with the highest antigenicity were produced as peptides, the immunogenicity of each peptide was determined by ELISA using sheep serum samples categorized as MVV positive (n=24) and negative (n=13). Subsequently, in house ELISA using above mentioned immunogenic peptides as antigen was used to investigate MVV seroprevalence in sheep (n=88). According to the results, among three peptides, two of them strongly reacted with MVV positive serum samples and the mean absorbance values detected among positive and negative serum samples were statistically significant, indicating that these peptides were immunogenic (P=0.016 and P=0.038). The third peptide also reacted with positive serum samples but the mean absorbance value was not statistically significant and this peptide was considered non-immunogenic (P=0.175). The immunogenic two peptides showed the same high sensitivity and specificity values of 91.60 and 92.80 according to the commercial kit. Moreover, MVV seroprevalence detected by peptide-ELISAs using CKQGSKE and CRPQGKAGHKG peptides as antigen was 3.40 % and 4.5 %, respectively. As a result, it was shown that these peptides can be successfully used for serological diagnosis of MVV.en_US
dc.identifier.citationKoçkaya, E. S., Can, H., Yaman, Y., Kandemir, Ç., Taşkın, T., Karakavuk, M. ve diğerleri. (2024). Newly developed peptide-ELISA successfully detected anti-IgG antibodies against Maedi-Visna virus in sheep. Veterinary Immunology and Immunopathology, 274, 1-7.en_US
dc.identifier.endpage7en_US
dc.identifier.issn0165-2427
dc.identifier.pmid39002364en_US
dc.identifier.scopus2-s2.0-85198045116en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0165242724000928?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/11468/28742
dc.identifier.volume274en_US
dc.identifier.wosWOS:001270376000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthorŞireli, Halit Deniz
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofVeterinary Immunology and Immunopathologyen_US
dc.relation.isversionof10.1016/j.vetimm.2024.110806en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMVVen_US
dc.subjectPeptide-ELISAen_US
dc.subjectSeroprevalenceen_US
dc.subjectSheepen_US
dc.subjectTürkiyeen_US
dc.titleNewly developed peptide-ELISA successfully detected anti-IgG antibodies against Maedi-Visna virus in sheepen_US
dc.typeArticleen_US

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