LC/MS-MS analysis of phenolic compounds in Hyoscyamus albus L. extract: In vitro antidiabetic activity, in silico molecular docking, and in vivo investigation against STZ-induced diabetic mice

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dc.authorid0000-0002-7946-219Xen_US
dc.authorid0000-0003-2866-635Xen_US
dc.authorid0000-0002-5366-3938en_US
dc.authorid0000-0002-4090-7227en_US
dc.authorid0000-0002-4999-5673en_US
dc.contributor.authorLekmine, Sabrina
dc.contributor.authorBenslama, Ouided
dc.contributor.authorKadi, Kenza
dc.contributor.authorGarcia, Antonio Ignacio Martín
dc.contributor.authorYılmaz, Mustafa Abdullah
dc.contributor.authorAkkal, Salah
dc.date.accessioned2023-09-29T11:30:31Z
dc.date.available2023-09-29T11:30:31Z
dc.date.issued2023en_US
dc.departmentDicle Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümüen_US
dc.description.abstractThis study aimed to investigate the chemical composition and antidiabetic properties of cultivated Hyoscyamus albus L. The ethanol extract was analyzed using LC-MS/MS, and 18 distinct phenolic compounds were identified. Among these, p-coumaric acid (6656.8 ± 3.4 µg/g), gallic acid (6516 ± 1.7 µg/g), luteolin (6251.9 ± 1.3 µg/g), apigenin (6209.9 ± 1.1 µg/g), and rutin (5213.9 ± 1.3 µg/g) were identified as the most abundant polyphenolic molecules. In the in vitro antidiabetic experiment, the ability of the plant extract to inhibit α-glucosidase and α-amylase activities was examined. The results indicated that the extract from H. albus L. exhibited a higher inhibitory effect on α-amylase compared to α-glucosidase, with an IC50 of 146.63 ± 1.1 µg/mL and 270.43 ± 1.1 µg/mL, respectively. Docking simulations revealed that luteolin, fisetin, and rutin exhibited the most promising inhibitory activity against both enzymes, as indicated by their high contrasting inhibition scores. To further investigate the in vivo antidiabetic effects of H. albus L., an experiment was conducted using STZ-induced diabetic mice. The results demonstrated that the plant extract effectively reduced the levels of cholesterol and triglycerides. These findings suggest that H. albus L. may have therapeutic potential for managing hyperlipidemia, a common complication associated with diabetes. This highlights its potential as a natural remedy for diabetes and related conditions.en_US
dc.identifier.citationLekmine, S., Benslama, O., Kadi, K., Garcia, A.I.M., Yılmaz, M. A. ve Akkal, S. (2023). LC/MS-MS analysis of phenolic compounds in Hyoscyamus albus L. extract: In vitro antidiabetic activity, in silico molecular docking, and in vivo investigation against STZ-induced diabetic mice. Pharmaceuticals, 16(7), 1-17.en_US
dc.identifier.doi10.3390/ph16071015
dc.identifier.endpage17en_US
dc.identifier.issn1424-8247
dc.identifier.issue7en_US
dc.identifier.pmid37513927
dc.identifier.scopus2-s2.0-85166228213
dc.identifier.scopusqualityQ2
dc.identifier.startpage1en_US
dc.identifier.urihttps://www.mdpi.com/1424-8247/16/7/1015
dc.identifier.urihttps://hdl.handle.net/11468/12634
dc.identifier.volume16en_US
dc.identifier.wosWOS:001039619900001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorYılmaz, Mustafa Abdullah
dc.language.isoenen_US
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)en_US
dc.relation.ispartofPharmaceuticals
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntidiabetic activityen_US
dc.subjectHyoscyamus albus L.en_US
dc.subjectα-glucosidaseen_US
dc.subjectα-amylaseen_US
dc.subjectMolecular dockingen_US
dc.titleLC/MS-MS analysis of phenolic compounds in Hyoscyamus albus L. extract: In vitro antidiabetic activity, in silico molecular docking, and in vivo investigation against STZ-induced diabetic miceen_US
dc.titleLC/MS-MS analysis of phenolic compounds in Hyoscyamus albus L. extract: In vitro antidiabetic activity, in silico molecular docking, and in vivo investigation against STZ-induced diabetic mice
dc.typeArticleen_US

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