Species Diversity and Pheno- and Genotypic Antibiotic Resistance Patterns of Staphylococci Isolated from Retail Ground Meats

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dc.contributor.authorGuran, Husnu Sahan
dc.contributor.authorKahya, Serpil
dc.date.accessioned2024-04-24T17:11:23Z
dc.date.available2024-04-24T17:11:23Z
dc.date.issued2015
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe presence and species diversity of staphylococci in 250 ground beef and lamb meat samples obtained from Diyarbakir, Turkey were investigated. The presence of the 16S rRNA gene, mecA, nuc, pvl, and femA was analyzed by multiplex PCR. Pheno- and genotypic antibiotic resistance profiles of 208 staphylococci isolates were established. Of the ground beef and ground lamb samples, 86.4% and 62.4% were positive for staphylococci, respectively. Staphylococcus aureus, S. saprophyticus, S. hominis, S. lentus, S. pasteuri, S. warneri, S. intermedius, and S. vitulinus made up 40.8%, 28.8%, 11%, 3.8%, 3.8%, 2.4%, 2.4%, and 2.4% of isolates, respectively. Of the 85 S. aureus isolates, 40%, 47%, and 5.8% carried femA, mecA, and pvl, respectively, whereas the corresponding rates for the 118 coagulase-negative staphylococci (CoNS) were 0%, 10.1%, and 0%, respectively. We determined from the 208 isolates, the highest antibiotic resistances were to tetracycline and oxytetracycline (85.5%), followed by penicillin (51.4%), novobiocin (45.6%), ampicillin (39.9%), and doxycycline (31.7%), using the Clinical and Laboratory Standards Inst. (CLSI) method. All isolates were sensitive to gentamycin, ofloxacin, and tobramycin, but 2.3% of the S. aureus isolates had resistance to vancomycin. The staphylococci isolates carried tet(K), blaZ, tet(L), tet(W), cat, tet(S), tet(M), ermB, ermA, and ermC antibiotic resistance genes at rates of 59%, 51.7%, 36.9%, 31.8%, 27.2%, 27.2%, 24.4%, 18.1%, 7.9%, and 3.9%, respectively. Practical Application Our study is the 1st to detect pvl in MRSA isolates from ground meat samples in Turkey. Moreover, we detected 2 instances of vancomycin resistance in MRSA isolates and also detected a high level of multidrug resistance in staphylococci isolates. The results of this study show that ground meat can be a source of staphylococci of concern to public health because of potential for pathogenicity and as a reservoir for antibiotic resistance genes.en_US
dc.identifier.doi10.1111/1750-3841.12893
dc.identifier.endpageM1298en_US
dc.identifier.issn0022-1147
dc.identifier.issn1750-3841
dc.identifier.issue6en_US
dc.identifier.pmid25944650
dc.identifier.scopus2-s2.0-84930930303
dc.identifier.scopusqualityQ1
dc.identifier.startpageM1291en_US
dc.identifier.urihttps://doi.org/10.1111/1750-3841.12893
dc.identifier.urihttps://hdl.handle.net/11468/17471
dc.identifier.volume80en_US
dc.identifier.wosWOS:000356375600023
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Food Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntibiotic Resistanceen_US
dc.subjectCoagulase-Negative Staphylococcien_US
dc.subjectGround Meaten_US
dc.subjectStaphylococcus Aureusen_US
dc.titleSpecies Diversity and Pheno- and Genotypic Antibiotic Resistance Patterns of Staphylococci Isolated from Retail Ground Meatsen_US
dc.titleSpecies Diversity and Pheno- and Genotypic Antibiotic Resistance Patterns of Staphylococci Isolated from Retail Ground Meats
dc.typeArticleen_US

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