Volatile and phenolic profiling of a traditional medicinal plant, Hypericum empetrifolium with in vitro biological activities

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dc.authorid0000-0003-4163-9962en_US
dc.contributor.authorBoğa, Mehmet
dc.contributor.authorErsoy, Ezgi
dc.contributor.authorÖzkan, Esra Eroğlu
dc.contributor.authorÇınar, Ercan
dc.contributor.authorKara, Emel Mataracı
dc.contributor.authorCantürk, Yeter Yeşil
dc.contributor.authorZengin, Gökhan
dc.date.accessioned2022-06-28T11:35:49Z
dc.date.available2022-06-28T11:35:49Z
dc.date.issued2021en_US
dc.departmentDicle Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümüen_US
dc.descriptionWOS:000632472300005
dc.descriptionPMID: 33600919
dc.description.abstractEthnopharmacological relevance: Hypericum empetrifolium Willd is a member of the Hypericaceae family, mainly known in southern Greece, and western Turkey. Being a centuries-old medicinal plant, the aerial parts of the plant have been used for the treatment of herpes, kidney stones, gastric ulcer, and also for their anti-helminthic, and diuretic purposes traditionally. Aim of the study: The current study aimed to investigate the phytochemical profiles of the essential oil, and two ethanol extracts prepared from the aerial parts (H. empetrifolium aerial parts extract HEA), and roots of the plant (H. empetrifolium roots extract HER), and to provide data on antioxidant, anticholinesterase, antityrosinase, antiurease, cytotoxic, and antimicrobial activities of the extracts. Materials and methods: In this study, volatile and phenolic compounds of the HEA and HER were analyzed by GC MS and LC-MS/MS, respectively. Antioxidant potential of the extracts was clarified by using DPPH radical scavenging assay, ABTS cation radical assay, and the CUPRAC assay. Acetylcholinesterase, butyrylcholinesterase, urease, and tyrosinase inhibitory activity assays were used to determine enzyme inhibition capacity of the extracts. Cytotoxic activity of the extracts was established by using XTT assay. The antimicrobial activity of the extracts was determined by the microbroth dilution technique. Results: The major compounds of the essential oil were revealed as alloaromodendrene (24.7%), alpha-pinene (14.7%), beta-pinene (10.7%), and alpha-terpineol (7.7%) by the GC-MS analysis. According to the LC-MS/MS analysis results, quinic acid was the most abundant constituent in both extracts with 20612.42 +/- 169.02 mu g/g extract in HEA extract, and with 2254.34 +/- 18.49 mu g/g extract in HER extract, respectively. The HEA extract was also found to be rich in terms of chlorogenic acid (5583.14 +/- 38.52 mu g/g extract), isoquercitrin (3076.77 +/- 40.92 mu g/g extract), and malic acid (2822.90 +/- 31.90 mu g/g extract). HEA extract exhibited a significant antioxidant effect with an IC50 value of 11.98 +/- 0.22 mu g/mL according to the DPPH radical scavenging assay. Similar results were obtained with the ABTS cation radical assay, and the CUPRAC assay. HER extract showed a strong butyrylcholinesterase inhibitory effect with 88.69 +/- 0.62% at 200 mu g/mL concentration. Both extracts were considered to have moderate anti-tyrosinase activity compared to the standard at 200 mu g/mL. The best antimicrobial activity was obtained for HEA against C. parapsilosis with an MIC value of 4.88 mu g/mL. HEA also exhibited antifungal activity against C. tropicalis with 19.53 mu g/mL. Only HER exhibited low cytotoxicity on A498 renal cell lines with 60.6% cell viability. Conclusion: Unquestionably, H. empetrifolium Willd has ethnopharmacological importance according to these results, and further investigations are required to evaluate other therapeutic properties of the plant.en_US
dc.identifier.citationBoğa, M., Ersoy, E., Özkan, E.E., Çınar, E., Kara, E.M., Cantürk, Y.Y. ve diğerleri. (2021). Volatile and phenolic profiling of a traditional medicinal plant, Hypericum empetrifolium with in vitro biological activities. Journal of Ethnopharmacology, 272, 1-10.en_US
dc.identifier.doi10.1016/j.jep.2021.113933
dc.identifier.endpage10en_US
dc.identifier.issn0378-8741
dc.identifier.issn1872-7573
dc.identifier.pmid33600919
dc.identifier.scopus2-s2.0-85101199865
dc.identifier.scopusqualityQ1
dc.identifier.startpage1en_US
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0378874121001598?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/11468/10074
dc.identifier.volume272en_US
dc.identifier.wosWOS:000632472300005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorBoğa, Mehmet
dc.language.isoenen_US
dc.publisherElsevier Ireland LTDen_US
dc.relation.ispartofJournal of Ethnopharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHypericum empetrifolium Willden_US
dc.subjectLC-MS/MSen_US
dc.subjectCytotoxicityen_US
dc.subjectAnticandidalen_US
dc.subjectTraditional usesen_US
dc.titleVolatile and phenolic profiling of a traditional medicinal plant, Hypericum empetrifolium with in vitro biological activitiesen_US
dc.titleVolatile and phenolic profiling of a traditional medicinal plant, Hypericum empetrifolium with in vitro biological activities
dc.typeArticleen_US

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