Antiglycative effect of combination of extracts of capsicum annuum (chilli) and pyridoxamine against glycation in streptozotocin-induced experimental diabetes in rats
Yükleniyor...
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Science and Business Media
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Glycation causes the formation of advanced glycation end products, which play an important role in the pathogenesis of
diabetic complications, which alter changes the structure and functional properties of proteins that negatively afect cellular
metabolism. In the current study, we investigated benefcial efects of red chili pepper extract and capsaicin, the main bitter
ingredient in red peppers and pyridoxamine, a vitamin B6, one by one or in combination on glycation and advanced glycation end products damage in streptozotocin-induced diabetic rats. The total amount of capcaicin in red chili pepper extract
was found to be 3.51 mg/g DW by HPLC. Male Sprague Dawley rats (8 weeks old), weighing 200–300 g, were divided into
12 groups of 8 rats each. A single dose of streptozotocin (50 mg/kg body weight) was administered to induce experimental
diabetes in rats. Group 1 was normal control, group 2 was diabetic control. Groups 3 and 4, 200 and 400 mg/kg red chili
pepper extract, groups 5 and 6, 6 and 8 mg/kg capsaicin and group 7, 4 mg/ml pyridoxamine and group 8 were receiving
300 mg/kg metformin. Groups 9 and 10, 200 and 400 mg/kg red chili pepper extract+4 mg/ml PM and groups 11 and 12
were diabetic rats treated with 6 and 8 mg/kg capsaicin+4 mg/ml pyridoxamine. Oral treatment with intragastric tube was
administered every day for 28 days. At the end of the experiment, subjects in all groups were given anesthesia once and
the rib cage was performed by intracardiac intervention and blood was collected. Immediately, the liver and kidneys were
grossly removed for histological examination. The results of our study showed that the combined doses of red chili pepper
extract+pyridoxamine and capsaicin+pyridoxamine signifcantly reduced blood glucose levels, serum uric acid, urea, creatinine, AST, ALT, ALP, LDH, total cholesterol, fructosamine, HbA1c levels and signifcantly increased serum HDL and
insulin levels. At the same time, histopathological studies also strongly supported the anti-diabetic, hepatoprotective and
renoprotective efects of these combination doses.
Açıklama
Anahtar Kelimeler
Diabetes, Advanced glycation end products, Diabetic nephropathy, Red chili pepper extract, Pyridoxamine, Capsaicin
Kaynak
Revista Brasileira de Farmacognosia
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
33
Sayı
4
Künye
İnceören, N., Akal, F., Nas, C., Deveci, E., Kızıl, G. ve Kızıl, M. (2023). Antiglycative effect of combination of extracts of capsicum annuum (chilli) and pyridoxamine against glycation in streptozotocin-induced experimental diabetes in rats. Revista Brasileira de Farmacognosia, 33(4), 831-846.
