GnRH and its receptor (GnRH-R) are expressed in the canine placenta and uterus

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dc.contributor.authorSchaefer-Somi, S.
dc.contributor.authorKowalewski, M. P.
dc.contributor.authorKanca, H.
dc.contributor.authorBozkurt, M. P.
dc.contributor.authorGram, A.
dc.contributor.authorSabitzer, S.
dc.contributor.authorKucukaslan, I.
dc.date.accessioned2024-04-24T16:18:29Z
dc.date.available2024-04-24T16:18:29Z
dc.date.issued2015
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn reproductive tissues, GnRH participates in the regulation of cell growth and proliferation by direct binding to the GnRH-R, which is essential for embryo implantation. However, there is no study on the expression and cellular localization of GnRH and GnRH-R in the canine uterus and placenta. Therefore, bitches were ovariohysterectomized 10 to 12 days after mating (vaginal cytology and progesterone measurement), the uteri were flushed, and if embryos were detectable, bitches were allocated to the embryo positive group (E-pos.; preimplantation, n = 5). Other bitches were operated at later stages and, dependent on the gestational age, either allotted to the post-implantation group (Day 18-25 after mating, n = 9), or the mid-gestation group (Day 30-40 after mating, n = 3). Dogs negative in embryo flushing served as controls (E-neg.; controls, n = 5). Samples of the entire uterine wall were taken from the middle of the horn in E-neg. and E-pos. groups, and from placental and interplacental uterine sites in post-implantation and mid-gestation groups. GnRH-R expression was localized at the mRNA and protein levels by immunohistochemistry and in situ hybridization. The expression of GnRH and GnRH-R mRNA was assessed by semi-quantitative polymerase chain reaction. Additionally, both GnRH and GnRH-R mRNA were expressed in all tissues examined until mid-gestation. Relative expression of GnRH was higher than that of GnRH-R (P < 0.05). During the post-implantation stage, GnRH-R expression was significantly higher in uteroplacental than in interplacental tissues. In the uterus, GnRH-R stained strongly in the surface and glandular epithelial cells, and seemed to be weaker in myometrium and stroma. Placental signals were predominantly localized in fetal trophoblast cells and to a lesser extent in maternal decidual cells. These findings suggest a local regulatory function of GnRH during early canine pregnancy. (C) 2015 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.theriogenology.2015.07.034
dc.identifier.endpage1489en_US
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.issue9en_US
dc.identifier.pmid26296524
dc.identifier.scopus2-s2.0-84946480497
dc.identifier.scopusqualityQ1
dc.identifier.startpage1482en_US
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2015.07.034
dc.identifier.urihttps://hdl.handle.net/11468/16123
dc.identifier.volume84en_US
dc.identifier.wosWOS:000364616100005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTheriogenology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDomestic Dog (Canis Familiaris)en_US
dc.subjectGnrhen_US
dc.subjectGnrh-Receptoren_US
dc.subjectUterusen_US
dc.subjectPlacentaen_US
dc.titleGnRH and its receptor (GnRH-R) are expressed in the canine placenta and uterusen_US
dc.titleGnRH and its receptor (GnRH-R) are expressed in the canine placenta and uterus
dc.typeArticleen_US

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