Deciphering the effect of Potentilla fulgens root extract against healthy HUVEC cell line and cancer cell lines (A549 and SKOV-3)

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dc.authorid0000-0003-0274-4479en_US
dc.authorid0000-0003-1756-9757en_US
dc.authorid0000-0003-1663-3403en_US
dc.authorid0000-0003-3755-5846en_US
dc.authorid0000-0002-8684-1390en_US
dc.contributor.authorİpek, Polat
dc.contributor.authorBaran, Ayşe
dc.contributor.authorBaran, Mehmet Fırat
dc.contributor.authorEftekhari, Aziz
dc.contributor.authorKhusro, Ameer
dc.contributor.authorOmmati, Mohammad Mehdi
dc.contributor.authorAliyev, Elvin
dc.contributor.authorKhalilov, Rovshan
dc.date.accessioned2024-10-22T13:09:15Z
dc.date.available2024-10-22T13:09:15Z
dc.date.issued2024en_US
dc.departmentDicle Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bililmleri Bölümüen_US
dc.description.abstractBackground: Potentilla fulgens, a highly valued indigenous medicinal herb grown in high altitudes of the Himalayan region with anticancer, hypoglycaemic, antibacterial, anti-inflammatory, and antiulcerogenic properties, are used in traditional systems of medicine. This study was aimed to investigate the effect of P. fulgens root extract, as one of the natural alternatives to chemotherapeutic drugs used in cancer treatment, on proliferation, apoptosis, and autophagy of human non-small cell lung cancer cell line (A549), human ovarian cancer cell line (SKOV-3), and healthy human umbilical vein endothelial cell line (HUVEC). Methods: Anti-proliferative effect was assessed by MTT assay. The expression of autophagy and apoptosis-related proteins was evaluated by western blotting. Total oxidant status (TOS) and total antioxidant capacity (TAC) test were determined using standard kit methods. Results: Our results showed that the extract inhibited proliferation of HUVEC, A549, and SKOV-3 cells in a dose-dependent manner. MTT assay analysis revealed that the extract significantly (P<0.05) induced mortality in HUVEC, A549, and SKOV-3 cells. Western blot results revealed increased expression of NF-κB after the extract treatment but led to the down-regulation in Beclin-1, Bax, extracellular-signal-related kinase 1 and 2, Sequestosome-1, and cleaved Casp-3 levels. Treatment groups showed an increase in TOS and TAC values in A549 and SKOV-3 cell lines, while HUVEC cell line showed an increase in TAC and a decrease in TOS values, compared to the control group. Conclusions: Our findings indicated that P. fulgens root extract inhibited the proliferation of healthy cells and cancer cells through cell cycle arrest, representing its limited application as therapeutic agent in cancer treatment.en_US
dc.description.sponsorshipDicle University VETERINER.21.002en_US
dc.identifier.citationİpek, P., Baran, A., Baran, M. F., Eftekhari, A., Khusro, A., Ommati, M. M. ve diğerleri. (2024). Deciphering the effect of Potentilla fulgens root extract against healthy HUVEC cell line and cancer cell lines (A549 and SKOV-3). Journal of King Saud University - Science, 36(10), 1-8.en_US
dc.identifier.endpage8en_US
dc.identifier.issn1018-3647
dc.identifier.issue10en_US
dc.identifier.scopus2-s2.0-85203491160
dc.identifier.scopusqualityQ1
dc.identifier.startpage1en_US
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1018364724003227?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/11468/28861
dc.identifier.volume36en_US
dc.identifier.wosWOS:001315709900001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorİpek, Polat
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofJournal of King Saud University - Science
dc.relation.isversionof10.1016/j.jksus.2024.103410en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApoptosisen_US
dc.subjectAutophagyen_US
dc.subjectBaxen_US
dc.subjectBeclinen_US
dc.subjectCanceren_US
dc.subjectP. fulgensen_US
dc.titleDeciphering the effect of Potentilla fulgens root extract against healthy HUVEC cell line and cancer cell lines (A549 and SKOV-3)en_US
dc.titleDeciphering the effect of Potentilla fulgens root extract against healthy HUVEC cell line and cancer cell lines (A549 and SKOV-3)
dc.typeArticleen_US

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