Identification of phenolic compounds, antioxidant activity and anti-cancer effects of the extract obtained from the shoots of Ornithogalum narbonense L.

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dc.contributor.authorKoyuncu, Ismail
dc.contributor.authorGonel, Ataman
dc.contributor.authorAkdag, Abdurrahman
dc.contributor.authorYilmaz, Mustafa Abdullah
dc.date.accessioned2024-04-24T17:17:57Z
dc.date.available2024-04-24T17:17:57Z
dc.date.issued2018
dc.departmentDicle Üniversitesien_US
dc.description.abstractThis study aimed to examine the anti-cancer and antioxidant properties and identify the phenolic content of methanolic extract obtained from the shoots of the Ornithogalum narbonense L. (OR) species, which is used for folk-medicine and food in the Sanliurfa region of Turkey. The antioxidant activity of the extract was investigated using total phenolics, flavonoids, ABTS and CUPRAC methods. Phenolic component analysis of the plant extract was performed by LCMS/MS. The anti-cancer property of OR extract was investigated on human colon (DLD-1), endometrium (ECC-1) cancer cells and embryonic kidney (HEK-293) cells. Cytotoxic effects were defined with MTT, apoptotic activity with DNA fragmentation ELISA and AO/EB fluorescent staining, the genotoxic effect with the comet assay and the intracellular oxidative status with TAS and TOS methods. As a result of the study, it was determined that OR extract showed an antioxidant effect, and as a result of the content analysis made with LC-MS/MS, phenolic components were determined, the most abundant being cosmosiin, followed by cinnamic acid, p-coumaric acid and quinic acid. OR extract showed cytotoxic activity on DLD-1 and ECC-1 cancer cells, while the cytotoxic effect on HEK-293 cells was determined to be low. It was determined that through OR extract, by increasing the intracellular amount of free radicals on cancer cells, led to DNA damage, which consequently led to apoptosis of the cancer cells.en_US
dc.description.sponsorshipHarran University (HUBAK) [13136]en_US
dc.description.sponsorshipThis study was supported by the research fund of Harran University (HUBAK). Project No: 13136.en_US
dc.identifier.doi10.14715/cmb/2018.64.1.14
dc.identifier.endpage83en_US
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue1en_US
dc.identifier.pmid29412798
dc.identifier.scopus2-s2.0-85041725320
dc.identifier.scopusqualityQ4
dc.identifier.startpage75en_US
dc.identifier.urihttps://doi.org/10.14715/cmb/2018.64.1.14
dc.identifier.urihttps://hdl.handle.net/11468/18471
dc.identifier.volume64en_US
dc.identifier.wosWOS:000425228400013
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherC M B Assocen_US
dc.relation.ispartofCellular and Molecular Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOrnithogalum Narbonense L.en_US
dc.subjectGenotoxicityen_US
dc.subjectCytotoxicityen_US
dc.subjectLc-Ms/Msen_US
dc.subjectApoptosisen_US
dc.titleIdentification of phenolic compounds, antioxidant activity and anti-cancer effects of the extract obtained from the shoots of Ornithogalum narbonense L.en_US
dc.titleIdentification of phenolic compounds, antioxidant activity and anti-cancer effects of the extract obtained from the shoots of Ornithogalum narbonense L.
dc.typeArticleen_US

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