The Immunohistochemical and Bioinformatics Analysis of the Placental Expressions of Vascular Cell Adhesion Protein 1 (VCAM-1) and High Mobility Group Box 1 (HMGB1) Proteins in Gestational Diabetic Mothers

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dc.authoridOglak, Suleyman Cemil/0000-0001-7634-3008
dc.authoridBolluk, Gokhan/0000-0002-3506-6806
dc.authoridYILMAZ, Emine Zeynep/0000-0002-3537-8340
dc.contributor.authorOglak, Suleyman Cemil
dc.contributor.authorAsir, Firat
dc.contributor.authorYilmaz, Emine Zeynep
dc.contributor.authorBolluk, Gokhan
dc.contributor.authorKorak, Tugcan
dc.contributor.authorAgacayak, Elif
dc.date.accessioned2025-02-22T14:08:58Z
dc.date.available2025-02-22T14:08:58Z
dc.date.issued2024
dc.departmentDicle Üniversitesien_US
dc.description.abstractObjective We aimed to examine both the expression levels of high mobility group box 1 (HMGB1) and vascular cell adhesion molecule-1 (VCAM-1) proteins in the placentas of pregnant women with gestational diabetes mellitus (GDM) and control groups by immunohistochemical (IHC) method. Material and methods An experimental case-control study was conducted, including 40 pregnant women complicated with GDM and 40 healthy pregnant women. Placental tissues obtained following cesarean delivery were subjected to routine tissue monitoring. The placental sections were stained with VCAM-1 and HMGB1 immunostains and subjected to IHC examination under a light microscope. H-score (HS) was used to evaluate the results of IHC staining by semi-quantitative analysis. Pathway analysis in Cytoscape software identified GDM-associated proteins within HMGB1 and VCAM-1 interaction networks, followed by GO analysis to explore associated biological processes. Results Placental HGMB1 expression was significantly increased in the GDM group compared to the control group (p<0.001). However, placental VCAM-1 expression was found to be statistically similar in GDM and control groups (p=0.584). The shared 19 proteins were identified between HMGB1 and GDM, and 13 between VCAM-1 and GDM, with notable GO biological process terms such as immune system activation for HMGB1 and interleukin-6 regulation for VCAM-1 associated with GDM. Conclusion We consider that GDM-related inflammation and oxidative stress may contribute to tissue damage and inflammation by increasing placental HMGB1 expression. The blockade of HMGB1 and its receptors might represent a promising therapeutic approach to control inflammation in GDM. Understanding the distinct roles of HMGB1 and VCAM-1 may provide valuable insights for the development of targeted therapies aimed at mitigating the inflammatory processes associated with GDM and improving maternal and fetal outcomes.en_US
dc.identifier.doi10.1055/a-2451-2223
dc.identifier.issn0948-2393
dc.identifier.issn1439-1651
dc.identifier.pmid39532125en_US
dc.identifier.scopus2-s2.0-85211714600en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.urihttps://doi.org/10.1055/a-2451-2223
dc.identifier.urihttps://hdl.handle.net/11468/29738
dc.identifier.wosWOS:001353389700001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherGeorg Thieme Verlag Kgen_US
dc.relation.ispartofZeitschrift Fur Geburtshilfe Und Neonatologieen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzKA_WOS_20250222
dc.subjectgestational dabetes mellitusen_US
dc.subjecthigh mobility group box 1en_US
dc.subjectvascular cell adhesion molecule-1en_US
dc.subjectimmunohistochemical analysisen_US
dc.titleThe Immunohistochemical and Bioinformatics Analysis of the Placental Expressions of Vascular Cell Adhesion Protein 1 (VCAM-1) and High Mobility Group Box 1 (HMGB1) Proteins in Gestational Diabetic Mothersen_US
dc.typeArticleen_US

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