Circulating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep

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dc.contributor.authorHitit, Mustafa
dc.contributor.authorKose, Mehmet
dc.contributor.authorKaya, Mehmet Salih
dc.contributor.authorKirbas, Mesut
dc.contributor.authorDursun, Sukru
dc.contributor.authorAlak, Ilyas
dc.contributor.authorAtli, Mehmet Osman
dc.date.accessioned2024-04-24T17:20:49Z
dc.date.available2024-04-24T17:20:49Z
dc.date.issued2022
dc.departmentDicle Üniversitesien_US
dc.description.abstractMicroRNA (miRNA) plays an important role in the control of gene expression and is implied in many biological functions, including embryo implantation and development. The aim was to assess plasma miRNA profiles during the peri-implantation and ascertain potential candidate miRNA markers for early pregnancy diagnosis in ovine plasma. The plasma samples were obtained from a total of 24 ewes on days 12 (pre-implantation; P12, n = 4), 16 (implantation; P16, n = 4) and 22 (post-implantation; P22, n = 4) after mating, and on their corresponding days of 12 (Pre-C; C12, n = 4), 16 (Imp-C; C16, n = 4) and 22 (Post-C; C22, n = 4) of the estrous cycle. The miRNA profiles in plasma were assessed by microarray technology. We detected the presence of 60 ovine-specific miRNAs in plasma samples. Of these miRNAs, 22 demonstrated a differential expression pattern, especially between the estrous cycle and early pregnancy, and targeted 521 genes. Two miRNAs (oar-miR-218a and oar-miR-1185-3p) were confirmed using RT-qPCR in the ovine plasma samples. Protein-protein interaction (PPI) network of target genes established six functional modules, of which modules 1 and 3 were enriched in the common GO terms, such as inflammatory response, defense response, and regulation of immune response. In contrast, module 2 was enriched in the developmental process involved in reproduction, embryo development, embryonic morphogenesis, and regulation of the developmental process. The results indicate that miRNAs profiles of plasma seemed to be modulated during the peri-implantation stage of pregnancy in ewes. Circulating miRNAs could be promising candidates for diagnosis in early ovine pregnancy.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK); [214O643]en_US
dc.description.sponsorshipThe study was financially funded by Scientific and Technological Research Council of Turkey (TUBITAK) projects (214O643) to MK. This study was partially submitted to the Proceedings of the 22nd Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR), Cordoba, Spain, 27-29 September 2018.en_US
dc.identifier.doi10.3389/fgene.2022.929477
dc.identifier.issn1664-8021
dc.identifier.pmid36061213
dc.identifier.scopus2-s2.0-85137252816
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.3389/fgene.2022.929477
dc.identifier.urihttps://hdl.handle.net/11468/19262
dc.identifier.volume13en_US
dc.identifier.wosWOS:000848520200001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherFrontiers Media Saen_US
dc.relation.ispartofFrontiers in Genetics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectOvineen_US
dc.subjectPlasmaen_US
dc.subjectMicrornaen_US
dc.subjectCirculatingen_US
dc.subjectEarly Pregnancyen_US
dc.subjectExpressionen_US
dc.titleCirculating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheepen_US
dc.titleCirculating miRNAs in maternal plasma as potential biomarkers of early pregnancy in sheep
dc.typeArticleen_US

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