Production of recombinant expansin and detection by SDS page analysis in Escherichia coli

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dc.authorid0000-0002-3612-9055en_US
dc.authorid0000-0002-3969-2900en_US
dc.authorid0000-0001-8097-9535en_US
dc.authorid0000-0001-6552-2713en_US
dc.contributor.authorGüneş, Serap
dc.contributor.authorEktiren, Demet
dc.contributor.authorKaraaslan, Mehmet
dc.contributor.authorVardin, Hasan
dc.date.accessioned2024-01-31T11:13:21Z
dc.date.available2024-01-31T11:13:21Z
dc.date.issued2023en_US
dc.departmentDicle Üniversitesi, Diyarbakır Tarım Meslek Yüksek Okulu, Gıda İşleme Bölümüen_US
dc.description.abstractThe study aims to produce Expansin protein isolated from a young tomato plant by using Escherichia coli which is used in recombinant protein production. Continuous culture is the most common method used to grow cells for recombinant protein production. In the study, the K12 strain of E. coli was used as a culture for the production of Expansin protein. The used LeExp1 gene was isolated from a young tomato plant. Since the related gene is found in very small amounts in plants, it has been reproduced using the PCR method and has been made workable with this method. T17 vector (T7 RNA polymerase system), which is frequently used in the production of recombinant protein, was used as the bacterial expression vector. The T7 RNA polymerase system is a commonly used vector in E. coli. With the transfer, the E. coli bacterium was given the ability to produce recombinant protein. Whether the obtained recombinant protein expressed the appropriate protein was determined by SDS Page analysis.en_US
dc.identifier.citationGüneş, S., Ektiren, D., Karaaslan, M. ve Vardin, H. (2023). Production of recombinant expansin and detection by SDS page analysis in Escherichia coli. International Journal of Agriculture, Environment and Food Sciences, 7(1), 117-121.en_US
dc.identifier.doi10.31015/jaefs.2023.1.13
dc.identifier.endpage121en_US
dc.identifier.issn2602-246X
dc.identifier.issn2618-5946
dc.identifier.issue1en_US
dc.identifier.startpage117en_US
dc.identifier.trdizinid1186710
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1186710
dc.identifier.urihttps://hdl.handle.net/11468/13319
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1186710
dc.identifier.volume7en_US
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorGüneş, Serap
dc.institutionauthorEktiren, Demet
dc.language.isoenen_US
dc.publisherGültekin Özdemiren_US
dc.relation.ispartofInternational Journal of Agriculture, Environment and Food Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectRecombinant proteinen_US
dc.subjectExpansin proteinen_US
dc.subjectLeExp1 geneen_US
dc.subjectEscherichia colien_US
dc.subjectPCRen_US
dc.titleProduction of recombinant expansin and detection by SDS page analysis in Escherichia colien_US
dc.titleProduction of recombinant expansin and detection by SDS page analysis in Escherichia coli
dc.typeArticleen_US

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