Production of recombinant expansin and detection by SDS page analysis in Escherichia coli
Yükleniyor...
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Gültekin Özdemir
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The study aims to produce Expansin protein isolated from a young tomato
plant by using Escherichia coli which is used in recombinant protein
production. Continuous culture is the most common method used to grow
cells for recombinant protein production. In the study, the K12 strain of E.
coli was used as a culture for the production of Expansin protein. The used
LeExp1 gene was isolated from a young tomato plant. Since the related
gene is found in very small amounts in plants, it has been reproduced using
the PCR method and has been made workable with this method. T17 vector
(T7 RNA polymerase system), which is frequently used in the production
of recombinant protein, was used as the bacterial expression vector. The
T7 RNA polymerase system is a commonly used vector in E. coli. With the
transfer, the E. coli bacterium was given the ability to produce recombinant
protein. Whether the obtained recombinant protein expressed the
appropriate protein was determined by SDS Page analysis.
Açıklama
Anahtar Kelimeler
Recombinant protein, Expansin protein, LeExp1 gene, Escherichia coli, PCR
Kaynak
International Journal of Agriculture, Environment and Food Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
7
Sayı
1
Künye
Güneş, S., Ektiren, D., Karaaslan, M. ve Vardin, H. (2023). Production of recombinant expansin and detection by SDS page analysis in Escherichia coli. International Journal of Agriculture, Environment and Food Sciences, 7(1), 117-121.
