Genome-guided investigation of secondary metabolites produced by a potential new strain Streptomyces BA2 isolated from an endemic plant rhizosphere in Turkey

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dc.authorid0000-0002-0255-2402en_US
dc.authorid0000-0002-9048-5798en_US
dc.contributor.authorKum, Ekrem
dc.contributor.authorİnce, Ebru
dc.date.accessioned2022-06-23T06:21:43Z
dc.date.available2022-06-23T06:21:43Z
dc.date.issued2021en_US
dc.departmentDicle Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Ana Bilim Dalıen_US
dc.descriptionWOS:000625600400005
dc.descriptionPMID: 33666690
dc.description.abstractTerrestrial actinomycetes are the important sources of secondary metabolites that serve as a major source of drugs. Recent advances in genome mining have revealed that Streptomyces genomes have a wide range of undiscovered secondary metabolite biosynthetic gene clusters. In the present study, genome mining was employed to discover biosynthetic potential of plant-associated strain Streptomyces BA2. Based on 16S rRNA gene sequencing, this strain was found to be closely related to Streptomyces durmitorensis, Streptomyces alboniger, and Streptomyces kanamyceticus with similarity of 99.71%, 99.64%, and 99.56%, respectively. The genome of BA2 contained 10.043.478 base pairs with G + C content of 69.92%. The annotation results revealed the presence of 9.056 protein coding genes, 88 tRNA and 18 rRNA genes. The dDDH and ANI values of genome sequences between strain BA2 and closely related type strains were considerably lower than the recommended threshold values. A total of 33 secondary metabolite biosynthetic gene clusters responsible for the biosynthesis of known and/or novel secondary metabolites, including non-ribosomal peptides, polyketides, terpenes, siderophores, bacteriocins, ectoines, and lassopeptides were identified. Metabolic profiling of Streptomyces sp. BA2 grown in three different culture media was determined by a non-targeted LC-MS/MS approach coupled with spectral networking. Significant bioactive natural products such as actinomycin D, desferrioxamine E, malyngamide K, and bouillonamide B were detected. Malyngamide K and bouillonamide B, known as marine cyanobacterial-derived compounds, were first reported from a Streptomyces strain in this study. Our study demonstrated the potentially novel strain Streptomyces sp. BA2 as a valuable source of new bioactive secondary metabolites.en_US
dc.identifier.citationKum, E. ve İnce, E. (2021). Genome-guided investigation of secondary metabolites produced by a potential new strain Streptomyces BA2 isolated from an endemic plant rhizosphere in Turkey. Archives of Microbiology, 203(5), 2431-2438.en_US
dc.identifier.doi10.1007/s00203-021-02210-z
dc.identifier.endpage2438en_US
dc.identifier.issn0302-8933
dc.identifier.issn1432-072X
dc.identifier.issue5en_US
dc.identifier.pmid33666690
dc.identifier.scopus2-s2.0-85102087769
dc.identifier.scopusqualityQ3
dc.identifier.startpage2431en_US
dc.identifier.urihttps://link.springer.com/content/pdf/10.1007/s00203-021-02210-z.pdf
dc.identifier.urihttps://hdl.handle.net/11468/10060
dc.identifier.volume203en_US
dc.identifier.wosWOS:000625600400005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKum, Ekrem
dc.institutionauthorİnce, Ebru
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofArchives of Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectStreptomycesen_US
dc.subjectMolecular identificationen_US
dc.subjectSecondary metabolitesen_US
dc.subjectGenome miningen_US
dc.subjectMetabolomicsen_US
dc.titleGenome-guided investigation of secondary metabolites produced by a potential new strain Streptomyces BA2 isolated from an endemic plant rhizosphere in Turkeyen_US
dc.titleGenome-guided investigation of secondary metabolites produced by a potential new strain Streptomyces BA2 isolated from an endemic plant rhizosphere in Turkey
dc.typeArticleen_US

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